Frequently Asked Questions

In RT-qPCR experiments, residual genomic DNA in RNA samples is the most hidden and destructive source of contamination. gDNA is highly homologous to cDNA sequences and will be non-specifically amplified by qPCR primers, leading to false positive results. Low-expression genes (with higher Ct values) are particularly susceptible to gDNA contamination interference. If amplification products appear in the control tube without reverse transcriptase, it indicates that residual gDNA must be removed from the RNA sample.

Traditional two-step methods require DNase I treatment of the RNA template first, then 85 degrees C heat inactivation, and finally adding reverse transcription components for reverse transcription, which is cumbersome and takes about 30 minutes. The one-step method completes gDNA removal and reverse transcription in the same tube simultaneously – simply add template RNA and water to start the reaction quickly, greatly simplifying operations and avoiding sample contamination and RNA degradation risks during complex pipetting.

Regular DNase I also has significant degradation activity against single-stranded DNA, which may affect primer and probe stability. The heat-labile dsDNase specifically digests double-stranded DNA with no activity against single-stranded DNA, primers, probes, or RNA, ensuring cDNA integrity. Additionally, this enzyme irreversibly inactivates within 5 minutes at 55 degrees C, requiring no additional heat inactivation step.

No. The red tracking dye in the product has been carefully optimized and will not interfere with fluorescence signal acquisition in downstream dye-based or probe-based qPCR, ensuring that amplification sensitivity and plateau are not affected.

This product is suitable for RNA templates from various species and sample types. The new-generation reverse transcriptase has excellent high-temperature stability and complex template read-through capability, particularly suitable for reverse transcription of RNA templates with high GC content, complex secondary structures, and low-concentration RNA samples.

The recommended RNA template amount is 500 ng to 1 microgram (total RNA). Verified testing shows that this product can stably amplify total RNA in the range of 10 pg to 1 microgram, suitable for low-abundance gene detection.

This can be verified by setting up a No-RT Control (No Reverse Transcriptase Control): omit the reverse transcriptase from the reaction system while adding all other components normally, then perform qPCR detection. If no amplification signal appears in the No-RT control tube, it indicates that gDNA has been successfully removed.

Long-term storage at -20 degrees C is recommended, with transport at <0 degrees C. After use, promptly return to -20 degrees C storage to maintain enzyme activity. Avoid repeated freeze-thaw; aliquoting is recommended.

The synthesized first-strand cDNA can be widely used in library construction, conventional PCR, qPCR (dye-based and probe-based), hybridization, and other molecular biology experiments.

This product can perform reverse transcription reactions in the 45-60 degrees C range. For RNA templates with complex secondary structures or high GC content, appropriately increasing the reaction temperature (e.g., 55 degrees C) is recommended to improve amplification efficiency and sensitivity.

This product is suitable for total RNA or mRNA templates from various sources including animal tissue, plant tissue, cultured cells, blood, viral samples, and more, covering mainstream application scenarios such as gene expression quantification and RNA virus detection.

The product pre-mixes a heat-labile dsDNase that specifically recognizes and degrades double-stranded DNA (dsDNA), including genomic DNA contamination, but has no effect on single-stranded DNA (ssDNA) or RNA. The heat-labile property allows this enzyme to rapidly inactivate during subsequent high-temperature reactions without affecting cDNA product integrity.

This product is a one-step All-in-Mix kit. Genomic DNA removal and cDNA synthesis are completed simultaneously in the same reaction tube, eliminating step-by-step operations, significantly shortening experiment time, reducing pipetting steps, and thereby lowering sample contamination risk and operational errors. Traditional two-step methods require gDNA removal first, then separate reverse transcription reactions, making the workflow more cumbersome.

The new-generation high-temperature reverse transcriptase YALEPIC Script Reverse Transcriptase Plus paired with this product has activity in the 50-60 degrees C range, with 55 degrees C as its optimal reaction temperature. High temperature conditions help unwind complex secondary structures of RNA templates, improving reverse transcription efficiency and cDNA length integrity.

Synthesized cDNA is recommended for short-term storage at -20 degrees C and long-term storage at -80 degrees C. Avoid repeated freeze-thaw to maintain the stability and reproducibility of qPCR quantification results.

No additional reverse transcription primers are needed. This product is an All-in-Mix pre-mixed formulation that already contains the key primer components needed for reverse transcription (such as Oligo(dT) and random primers).

This product’s buffer system has been optimized with a certain tolerance for common PCR inhibitors. However, using high-quality RNA templates (e.g., OD260/280 ratio between 1.8-2.0) is still recommended to ensure optimal reverse transcription efficiency and cDNA yield.

Yes. The cDNA synthesized by this product is suitable for both dye-based and probe-based qPCR detection. For probe-based multiplex qPCR, design and optimize the multiplex reaction system based on the specific qPCR instrument configuration and probe labeling requirements.

The product requires cold chain transport at <=0 degrees C. Long-term storage requires -20 degrees C. Avoid repeated freeze-thaw and prolonged room temperature placement to ensure enzyme activity and product stability.

Under strict storage at -20 degrees C and avoiding repeated freeze-thaw, please refer to the product packaging label for the specific expiration date.

The one-step gDNA removal and first-strand cDNA synthesis kit is an innovative product that simultaneously completes genomic DNA (gDNA) removal and first-strand cDNA synthesis in the same reaction system. Traditional two-step methods require DNase I treatment for gDNA removal first, followed by RNA purification and cDNA synthesis, which is cumbersome and prone to RNA degradation and loss. This product uses a special gDNA Remover with DNA degradation activity (heat-labile dsDNase) that works in concert with a new-generation reverse transcriptase. gDNA removal and cDNA synthesis are completed simultaneously with one-step addition, greatly simplifying the workflow, avoiding sample cross-contamination and RNA degradation risks from multiple pipetting steps, particularly suitable for high-throughput gene expression analysis and trace RNA sample processing.

This kit uses the new-generation YALEPIC Script reverse transcriptase, which can synthesize first-strand cDNA up to 15 kb long in the 50-60 degrees C range (optimal 55 degrees C). High-temperature reverse transcription conditions effectively unwind RNA template secondary structures, making it particularly suitable for reverse transcription of complex RNA templates with high GC content and rich secondary structures (such as RNA with hairpin structures and advanced folding). Compared to conventional reverse transcriptases that react at 37 or 42 degrees C, this product has superior thermal stability, with significantly improved cDNA yield and full-length proportion for complex templates.

No. The heat-labile dsDNase used in this kit specifically degrades double-stranded DNA (genomic DNA) with no activity against single-stranded RNA and single-stranded cDNA. This enzyme rapidly and irreversibly inactivates at 55 degrees C, automatically losing activity after completing gDNA removal. After the reaction, heating at 85 degrees C for 2-5 minutes completely inactivates all enzyme components without any interference with cDNA product stability or subsequent qPCR detection. cDNA processed by this product can be stably stored long-term, qPCR quantification results are reliable, and primer design does not require spanning exon junctions, simplifying experimental design.

This kit provides Oligo(dT)20 and Random Hexamers (N6 random primers) as separate components. Users can flexibly choose or combine them based on experimental needs: Oligo(dT)20 specifically binds to the poly(A) tail of mRNA, suitable for detecting eukaryotic mRNA with poly(A) structure; Random Hexamers randomly bind to various regions of the RNA template, suitable for total RNA, prokaryotic RNA, viral RNA, and non-coding RNA (lncRNA, ncRNA) reverse transcription. Additionally, users can use gene-specific primers (GSP) instead of the above primers for targeted gene reverse transcription. For qPCR detection, mixing Oligo(dT) and Random primers is recommended for more comprehensive cDNA coverage.

The optimal RNA template range for this product is 50 ng to 2 microgram total RNA (20 microL reaction system). If total RNA exceeds 2 microgram, scale up the reaction system proportionally. Due to the high-efficiency performance of the gDNA Remover and reverse transcriptase, the product has excellent compatibility with low-concentration RNA templates – even when the RNA template volume reaches 75%-80% of the total reaction volume, stable and effective reverse transcription efficiency can still be maintained. Therefore, this kit is particularly suitable for gene expression analysis of trace RNA samples (such as small numbers of cells from laser microdissection, rare tissue samples).

The synthesized first-strand cDNA has a wide range of applications: RT-qPCR – compatible with both SYBR Green dye-based and TaqMan probe-based quantitative PCR, with accurate and reliable results; RT-PCR – can be used for conventional PCR amplification of target gene fragments; cDNA library construction – can synthesize full-length cDNA up to 15 kb, suitable for library construction; Gene expression analysis – suitable for both high-copy and low-copy gene detection; Primer extension – for transcription start site analysis; SAGE – for serial analysis of gene expression. The cDNA synthesized by this product can be used for both conventional PCR and qPCR detection, and is particularly recommended for qPCR requiring high-sensitivity quantitative analysis.

This product is recommended for long-term storage at -20 degrees C, avoiding repeated freeze-thaw. Before first use, fully thaw all components, mix well, and centrifuge to collect at the tube bottom before aliquoting. Repeated freeze-thaw causes enzyme activity degradation, affecting reaction efficiency. Aliquoting into small portions (e.g., 10 reactions per aliquot) is recommended, using one aliquot each time. Transport conditions: dry ice or ice pack transport (<0 degrees C), ensuring the product maintains low temperature during transport. Under recommended storage conditions, the shelf life is 12 months.

High Ct values or no signal in qPCR detection typically have the following possible causes and solutions: Poor RNA template quality (degraded or low purity) – use high-quality RNA samples, check A260/A280 and A260/A230 ratios, ensure integrity. RNA template amount too low – adjust RNA input to 50 ng to 2 microgram range. RNA template amount too high (>2 microgram) – scale up the reaction system proportionally, or dilute RNA template appropriately. Improper reverse transcription temperature or time – ensure recommended reaction conditions: 55 degrees C incubation for 15-30 minutes (qPCR) or 30-60 minutes (PCR). qPCR primer design issues – insufficient primer specificity or Tm mismatch; redesign or verify primer specificity. Reaction system not fully mixed – gently flick to mix or briefly vortex after preparation, briefly centrifuge to collect reaction mixture. If problems persist, set up a No-RT Control (without reverse transcriptase) to rule out false positive signals from genomic DNA contamination.

Compared to major competitors, YALI’s core differentiation is that primers are provided as separate individual components. Users can freely adjust the ratio of Oligo(dT) and Random primers, or even replace them with gene-specific primers (GSP), offering flexibility far exceeding competitor pre-mixed formats. This design is particularly suitable for professional research scenarios requiring precise control of primer ratios.

Yes, the reverse transcription MasterMix in this product already contains a highly efficient RNase inhibitor that effectively protects RNA templates from degradation throughout the reaction, ensuring cDNA synthesis integrity and yield. To further ensure RNA stability, users should note the following during experimental operations: Handle on ice throughout – low temperature inhibits RNase activity; Use RNase-free tips, centrifuge tubes, and other consumables; Wear masks and gloves during operation to avoid RNase contamination; RNA samples should avoid repeated freeze-thaw; aliquoting is recommended.

Suitable for RT-PCR, RT-qPCR gene expression analysis, full-length cDNA cloning, RNA-seq library construction, hybridization, and other downstream applications. Particularly suitable for reverse transcription of RNA templates with complex secondary structures and low-abundance genes.

Script X Reverse Transcriptase is a novel variant engineered on the basis of M-MLV (RNase H-), with significantly improved thermal stability. It can effectively unwind complex RNA secondary structures at higher reaction temperatures, enhancing template affinity and processivity, while having higher impurity tolerance. Full-length cDNA synthesis capability reaches 15 kb, far superior to conventional M-MLV enzyme.

Wide template starting range: 1 pg to 5 microgram total RNA. Both trace samples and regular RNA samples are compatible, particularly suitable for low-abundance gene expression analysis.

Yes. The first-strand cDNA synthesis reaction mixture can be used directly as PCR or qPCR template without purification. It is recommended that the synthesis reaction mixture not exceed 1/10 of the total PCR reaction system to ensure optimal amplification efficiency.

Yes. Script X Reverse Transcriptase has high tolerance to common reverse transcription inhibitors (such as ethanol, guanidine salts, heparin, etc.). Even when RNA samples contain small amounts of impurities, efficient reverse transcription performance can be maintained, reducing RNA purification steps and saving time and cost.

Oligo(dT)20: specifically binds to the poly(A) tail of mRNA, suitable for analyzing mRNA expression and full-length cDNA synthesis. Random primers: randomly bind to multiple sites on the RNA template, suitable for RNA without poly(A) tails (such as bacterial RNA) or analyzing all regions of RNA. The kit provides Oligo(dT)20 and random primers as separate components. Users can flexibly choose or mix them based on experimental needs.

Through the optimized Script X Reverse Transcriptase, full-length cDNA up to 15 kb can be synthesized, suitable for long gene cloning and full-length transcript analysis.

Storage: long-term storage at -20 degrees C. Aliquoting is recommended to avoid repeated freeze-thaw. Transport: below 0 degrees C with ice packs or dry ice. When removing for use, handle on ice to prevent enzyme activity degradation.

The YALEPIC Script X series is a high cost-effectiveness universal first-strand cDNA synthesis kit, focusing on compatibility with a wide range of RNA templates and efficient reverse transcription of complex secondary structure RNA. The company also offers a one-step gDNA removal and first-strand cDNA synthesis kit for experimental needs requiring simultaneous removal of genomic DNA contamination. Users can select the appropriate product based on downstream application requirements.

The kit’s Mix contains an optimized gDNA removal component that degrades genomic DNA in situ during the reverse transcription pre-incubation step. For routine total RNA samples, no additional DNase treatment is needed. For crude RNA with high gDNA contamination, extending the pre-incubation time will thoroughly remove contaminants, preventing non-specific genomic amplification in qPCR.

With the proprietary RNase H-minus reverse transcriptase, the kit can synthesize up to 15 kb full-length cDNA under optimal conditions, and 8-10 kb under standard conditions, with no RNA hydrolysis side effects. It is suitable for large-fragment gene cloning and full-length cDNA library construction.

The kit contains pre-mixed Oligo dT + random hexamer primers, suitable for most mRNA reverse transcription. For gene-specific reverse transcription, you can substitute with your own gene-specific primers without affecting gDNA removal or reverse transcriptase activity.

Yes. The kit’s product has undergone thorough gDNA removal and is compatible with both standard agarose gel PCR amplification and SYBR Green qPCR quantification. It is a versatile one-tube cDNA reagent for multiple downstream applications.

The standard single reaction volume is 20 microL. The 100rxns specification provides 100 standard 20 microL reverse transcription reactions. The reaction volume can be scaled proportionally as needed.

The reverse transcriptase has strong thermostability and maintains good efficiency with slightly degraded RNA. For severely degraded RNA, we recommend re-extracting qualified total RNA to ensure cDNA yield and qPCR reproducibility.

Unopened, stored at -20 degrees C in the dark, the shelf life is 12 months. Repeated freeze-thaw reduces enzyme activity. We recommend aliquoting into smaller portions upon receipt to minimize freeze-thaw cycles.

It is compatible with total RNA from animal and plant cells, bacteria, fungi, insects, and other species. For prokaryotic RNA without polyA tails, the pre-mixed random primers efficiently initiate cDNA synthesis.

Fresh product can be stored at 4 degrees C for 1-3 days. For long-term storage, aliquot and store at -20 degrees C or -80 degrees C, avoiding repeated freeze-thaw degradation.

The optimized formulation efficiently synthesizes cDNA from as little as tens of nanograms of total RNA, suitable for single-cell trace RNA reverse transcription and low-abundance gene qPCR detection.

Short-term room temperature thawing followed by prompt return to -20 degrees C does not affect activity. If left at room temperature for more than 12 hours, we recommend running a small-scale pilot experiment to verify enzyme activity.

Not recommended for RNA molecular weight determination. Double-stranded DNA in the Marker maintains its double-stranded structure in standard agarose gel electrophoresis, while RNA migrates differently than DNA of the same size, leading to inaccurate size estimation. For rough observation of RNA band distribution, it can be used temporarily, but not for precise quantification. For denaturing gel electrophoresis, DNA strands will denature and the Marker absolutely should not be used for RNA size indication.

Common causes: (1) Stale electrophoresis buffer – after multiple uses, ion strength decreases and pH rises, reducing buffering capacity; replace with fresh buffer each time. (2) Nuclease contamination – use sterilized tips, tighten caps after use. (3) Gel quality issues – use high-quality agarose and ensure even solidification. (4) Excessive voltage – recommend 4-10 V/cm; higher voltage generates excess heat causing band diffusion.

This is a ready-to-use product containing 1x Loading Buffer – no additional addition or heating needed. Standard conditions: for well widths <6 mm, load 5 microL directly; for wider wells, increase to 8-10 microL. Recommended gel: 1%-2% agarose, voltage 4-10 V/cm, electrophoresis 25-60 minutes.

(1) More bands: 11 bands covering 100bp-15kb, vs 7-8 bands in most competitors, for higher resolution. (2) Dual indicator design: 500bp bright band + 1500bp secondary bright band for quick positioning and concentration estimation. (3) Ready-to-use: pre-mixed Loading Buffer, no preparation needed. (4) Concentration gradient design: differentiated band concentrations for semi-quantitative analysis.

2-8 degrees C for 2 years, -20 degrees C for 3 years. For frequent use, store at 2-8 degrees C; for long-term storage, use -20 degrees C. To avoid band degradation from repeated freeze-thaw, aliquot into small single-use portions after first use.

The 15K Marker covers 100bp-15kb, suitable for medium-to-large DNA fragment detection (plasmid digest analysis, large PCR product identification). For fragments 100bp-2kb, choose the 2K Marker for higher resolution. For fragments above 15kb, choose 10K or higher specification products. Select the product that best matches your target DNA fragment size for optimal resolution.

The product design has 500bp at 100 ng/5 microL (bright), 1500bp at 75 ng/5 microL (secondary bright), others at 40-50 ng/5 microL. This differentiated design aids quick positioning and semi-quantification. If unexpected unevenness occurs, check: accurate loading volume (5 microL recommended), appropriate gel concentration (1% agarose recommended), and fresh buffer.

Compatible. The YALEPIC 15K DNA Marker works with GelRed, SYBR Green, Goldview, and other common nucleic acid fluorescent dyes. Note: different dyes have varying staining efficiencies that may affect band brightness. When using a new dye type, optimize exposure parameters with a pilot experiment. For EB staining, use 0.5 microgram/mL EB solution for 15-20 minutes.

Normal product should be a clear, transparent solution. If precipitate is found, possible causes: (1) SDS precipitation from temperature fluctuations during transport; (2) Trace precipitate after long-term storage. Handling: place at room temperature or warm gently at 37 degrees C for 5-10 minutes, mix by gentle inversion. Do not vortex or boil, as this may denature DNA.

Typical applications: (1) PCR product fragment size verification; (2) Plasmid DNA restriction digest identification – confirming recombinant plasmid construction through digest fragment sizes; (3) Genomic DNA restriction fingerprinting – Southern blot pre-hybridization fragment size screening; (4) DNA fragment quality assessment before gel recovery – determining target band position for excision; (5) Ligation and digestion product fragment identification and semi-quantitative concentration analysis.

Traditional EB (ethidium bromide) is a strong mutagen, classified as a highly carcinogenic hazardous substance, requiring full protective equipment and specific waste disposal areas. YALEPIC Gel Redness uses a unique macromolecular oil-based technology with a molecular weight of approximately 1400, which cannot penetrate living cell membranes. The Ames Test has confirmed that this dye is completely non-mutagenic at gel staining concentrations, with biosafety far exceeding that of EB, lower waste disposal costs, and a more friendly profile for laboratory personnel and the environment.

Yes, no equipment replacement is needed at all. YALEPIC Gel Redness has identical spectral properties to traditional EB stain, with excitation at approximately 300 nm UV wavelength and emission at approximately 600 nm. Your existing gel imaging system, UV transilluminator, and filters require no adjustments, enabling a zero-cost smooth transition from EB to a safe dye.

Almost no effect. After binding to nucleic acids in the gel, this dye does not significantly increase the molecular weight burden of nucleic acids, causing minimal interference with electrophoretic migration of fragments of all sizes, ensuring accurate band positions. Additionally, due to its extremely low non-specific binding to free nucleic acids, background noise is well controlled. Combined with excellent sensitivity, high-contrast, low-background clear gel imaging results can be easily obtained.

Both methods are supported. For convenience and efficiency, in-gel staining is recommended, where the dye is added directly during gel preparation (no need to wait for the gel to cool), completing the process in one step. For quantitative analysis of post-electrophoresis gels or subsequent gel recovery experiments, post-staining (immersing the gel in diluted dye after electrophoresis) may be more appropriate, as it avoids the dye’s impact on nucleic acid recovery efficiency. Please choose the most suitable method based on your specific experimental needs and workflow.

Yes. YALEPIC Gel Redness has excellent staining performance for double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA. Its high sensitivity makes it suitable for detecting RNA samples at various concentrations in agarose gel or polyacrylamide gel electrophoresis.

This product is available in multiple specifications to meet different experimental needs. The current specifications are: Catalog number YDG19019, 500 microL, 450.00 CNY; YDG19019-05, 5×1 ml, 3800.00 CNY. For ordering or consultation, please call 0512-66256010 or email info@yalibiotech.com. The YALI Biotech sales team will provide professional product selection guidance and technical support.

The diluted working solution should be prepared fresh and used immediately. Store in the dark and use within 3 days for short-term storage. Long-term storage is not recommended. The stock solution is stable when sealed within its shelf life.

(1) Dye concentration too high – reduce the addition amount; (2) Nucleic acid overload – reduce the loading volume; (3) Expired buffer – replace with fresh TAE/TBE electrophoresis buffer. For tailing issues, switching to post-staining can improve results.

The YALEPIC 100bp Plus DNA Marker has a wider band coverage (100-2000 bp, 10 bands) compared to the regular 100bp Marker (100-1000 bp). The 500 bp band is designed as an enhanced bright band (100 ng/5 microL) for quick positioning and semi-quantitative analysis. Additionally, the product is pre-mixed with 1x Loading Buffer, ready to use.

Tailing or blurring is typically caused by: Stale electrophoresis buffer – replace with fresh 1x TAE or TBE. Inappropriate gel concentration – use 1-1.5% agarose for 100bp Marker. Excessive loading volume – use 5 microL per lane as recommended. DNA degradation – avoid nuclease contamination, use nuclease-free consumables. After multiple uses, buffer ion strength decreases and pH rises, causing blurry or irregular migration – use fresh buffer each time.

Normal. The 500 bp band is specifically designed as an enhanced bright band (100 ng/5 microL), approximately 2x the concentration of other bands (~50-40 ng/5 microL). This design provides a clear reference for quick positioning and accurate quantification. Other bands are slightly dimmer but clearly visible. This is a widely adopted design in similar products.

Not recommended. This product is designed and optimized for agarose gel electrophoresis, not recommended for polyacrylamide gel electrophoresis (PAGE). For PAGE analysis, use a specialized non-denaturing PAGE DNA Marker. This product also cannot be used for RNA analysis in denaturing gels, as double-stranded DNA will denature to single strands under denaturing conditions, making it inaccurate for RNA molecular weight indication.

YALEPIC 100bp Plus DNA Marker supports two storage methods: 2-8 degrees C refrigerated for 2 years, -20 degrees C frozen for 3 years. Practical recommendations: Frequent use (weekly): store at 2-8 degrees C for convenience. Long-term storage (backup stock): store at -20 degrees C to maximize shelf life. Thaw and mix thoroughly before use. Avoid repeated freeze-thaw; aliquot for best stability.

Compatible. The YALEPIC 100bp Plus DNA Marker works with common nucleic acid fluorescent dyes including GelRed, SYBR Green, and GoldView. After electrophoresis, observe under the corresponding excitation light in a gel documentation system. Mix DNA Marker with dye before loading, or stain the gel post-electrophoresis – both methods produce clear band images.

This is a ready-to-use product, pre-mixed with 1x Loading Buffer – no additional addition needed. The optimized Loading Buffer formula ensures DNA samples sink into the well during electrophoresis and forms a clear tracking front for monitoring electrophoresis progress.

Common causes and solutions: Inappropriate agarose concentration – use 1%-1.5% for 100-2000 bp fragments; too low reduces small fragment resolution, too high slows electrophoresis. Uneven gel preparation – ensure agarose is completely dissolved and mixed before pouring. Excessive voltage – causes gel heating and reduces resolution; maintain 5-8 V/cm. Improper loading volume – use exactly 5 microL; excess causes band tailing. Use freshly prepared gels and regularly replace electrophoresis buffer for best separation.

Designed for agarose gel electrophoresis, not recommended for PAGE. As a ready-to-use product pre-mixed with 1x Loading Buffer, load 5 microL directly per lane. For wider wells, increase to 10 microL for adequate band brightness.

Consists of 8 double-stranded DNA fragments: 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 8000 bp, 10000 bp. Band concentrations: 4000 bp at 100 ng/5 microL (bright indicator), 6000 bp at 40 ng/5 microL (slightly dimmer), remaining 6 bands at 50 ng/5 microL. This configuration facilitates both fragment size positioning and semi-quantitative analysis.

Recommended 1% agarose gel for effective separation of 1000-10000 bp range. For higher resolution in the low molecular weight region, increase to 1.5%-2%. Excessive concentration impedes large fragment migration; too low results in insufficient small fragment separation.

Possible causes: (1) Electrophoresis buffer overused – reduced ion strength and buffering capacity; use fresh TAE or TBE each time. (2) Excessive voltage (>20 V/cm) causing band diffusion. (3) Insufficient or excessive loading volume. (4) Mismatched agarose concentration and target fragment size. Follow the recommended 5 microL loading and 1% gel concentration, and control voltage appropriately.

Small fragment weakening is common because small DNA fragments migrate faster and may reach the dye front where dye-DNA binding may dissociate, reducing fluorescence. Recommendations: (1) Use fresh electrophoresis buffer with proper ion strength; (2) Appropriately shorten electrophoresis time or reduce voltage; (3) Add EB or equivalent dye to the gel at 0.5 microgram/mL; (4) Use a higher-resolution gel documentation system.

Not recommended. The molecular weight range (1000-10000 bp) achieves ideal separation in agarose gels. In non-denaturing PAGE, DNA fragments in this range exhibit significantly different migration behavior than in agarose, making band separation unreliable. Use a PAGE-optimized DNA Marker for polyacrylamide gels.

Store at 2-8 degrees C protected from light for 2 years; -20 degrees C for 3 years. Aliquot into small single-use portions after first use to avoid repeated freeze-thaw of the entire tube. Repeated freeze-thaw may cause DNA strand breakage or degradation, affecting band clarity and concentration accuracy. Working solution at 2-8 degrees C should be used within 4 weeks.

Prepared by restriction digestion of plasmid DNA, providing more accurate molecular weight positioning, cleaner background, and no non-specific band interference compared to traditional PCR product-mixed markers. Pre-mixed 1x Loading Buffer, ready to use. The 4000 bp super-bright indicator band (100 ng/5 microL) has extremely high visibility for quick positioning. YALI Biotech also offers highly competitive market pricing.

YALEPIC 1Kb DNA Marker undergoes strict nuclease inactivation during manufacturing and contains no residual nuclease activity. It serves only as an electrophoresis molecular weight reference and will not degrade co-loaded sample DNA. However, use dedicated pipette tips and loading buffers and follow standard electrophoresis practices to avoid cross-contamination.

Tailing or smiling effects are typically related to: (1) Uneven gel preparation or loading before complete solidification; (2) Buffer level not covering gel surface; (3) Unstable voltage or excessive current; (4) Overloading. Recommendations: ensure gel is fully solidified before removing comb; buffer should cover gel surface by 1-2 mm; maintain constant voltage 80-120 V; load 5 microL as recommended, avoid overloading.

Yes, this is a ready-to-use product, pre-mixed with 1x Loading Buffer. Simply load 5 microL directly for agarose gel electrophoresis – very convenient and quick.

This product consists of 10 double-stranded DNA fragments: 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, 5000 bp, 8000 bp. The 750 bp band is at 100 ng/5 microL (bright band); 1500 bp at 40 ng/5 microL; all other bands at 50 ng/5 microL.

This product is suitable for agarose gel electrophoresis, not recommended for polyacrylamide gel electrophoresis (PAGE). For high-resolution double-stranded DNA analysis in polyacrylamide gels, use a dedicated PAGE DNA Marker.

Store at 2-8 degrees C for 2 years, or -20 degrees C for 3 years. Avoid repeated freeze-thaw. Do not heat. Transport with ice packs.

Common causes and solutions: (1) Stale electrophoresis buffer – reduced ion concentration and elevated pH cause blurry bands; replace with fresh buffer. (2) Improper gel preparation – ensure agarose is fully dissolved; allow gel to set completely (30-60 minutes). (3) Improper loading volume – try diluting 2-3x with water and loading 8-10 microL.

Yes, compatible with GelRed, SYBR Green, EB, and other common DNA fluorescent dyes in standard agarose gel electrophoresis staining systems.

Band degradation or tailing is typically caused by nuclease contamination. Use nuclease-free pipette tips and centrifuge tubes for aliquoting, avoid repeated freeze-thaw, and maintain aseptic technique during operation.

This product is prepared by restriction digestion of plasmid DNA, providing higher molecular weight accuracy, clearer and sharper bands, cleaner background, and excellent batch-to-batch stability compared to PCR product-mixed markers. The ready-to-use pre-mixed Loading Buffer design also saves preparation time.

Not recommended. DNA Markers are double-stranded DNA molecules; in RNA denaturing gel electrophoresis, double-stranded structures will denature, making size indication inaccurate. For RNA molecular weight reference, use a dedicated RNA Marker. However, for rough observation in standard agarose gel electrophoresis, it can be used approximately.

Suitable for PCR product identification, restriction fragment size analysis, genomic DNA fragment estimation, plasmid DNA identification, and other common molecular biology experiments. Covers a wide molecular weight range of 100-8000 bp, meeting most routine DNA fragment analysis needs.

This product is designed for agarose gel electrophoresis, not recommended for PAGE. Under 1% agarose gel at 6-8 V/cm for 20-40 minutes, band separation is optimal. PAGE has higher resolution but different DNA migration characteristics, potentially leading to inaccurate fragment size determination.

This is a ready-to-use product, pre-mixed with 1x Loading Buffer. Load 5 microL directly per electrophoresis lane – no additional preparation needed. For smaller gels or narrow wells, adjust to 2-3 microL as needed.

The 750 bp band is designed as an enhanced reference band at 100 ng/5 microL, while other bands are 50 ng/5 microL. This design facilitates quick reference band identification and semi-quantitative analysis of target DNA fragments. For accurate quantification, compare target band brightness with the 750 bp enhanced band.

Common causes and solutions: Inappropriate agarose concentration – use 1% gel; too high or low affects resolution. Uneven gel or excessive bubbles – re-prepare gel, ensure complete dissolution and no bubbles. Excessive voltage – use moderate 6-8 V/cm to avoid heat-induced band diffusion. Repeated freeze-thaw degradation – aliquot to avoid. Excessive loading – maintain 5 microL; excess causes band spreading or cross-contamination between lanes.

Both have identical band composition (100bp/250bp/500bp/750bp/1000bp/2000bp, 6 bands), similar specifications and ready-to-use design. Main differences: Manufacturing process – YALEPIC 2K uses restriction digestion of plasmid DNA, providing higher band clarity and batch stability. Brand positioning – YALI Biotech offers a more cost-effective option. They can be used interchangeably without protocol adjustments.

Store at 2-8 degrees C for 24 months, or -20 degrees C for 36 months. Choose based on usage frequency: frequent use – store at 4 degrees C; long-term unused – aliquot and store at -20 degrees C to avoid repeated freeze-thaw DNA degradation.

Yes, for semi-quantitative analysis. The 750 bp enhanced band is at 100 ng/5 microL, others at 50 ng/5 microL. Estimate target DNA concentration by comparing band brightness between sample and Marker lanes. For precise quantification, use fluorometric methods (Qubit or qPCR).

Common situations: All bands dim – electrophoresis time too long causing diffusion, or insufficient dye staining. Only bottom two bands bright, upper bands weak – Marker degradation; replace with fresh Marker. Uneven brightness – 750 bp being significantly brighter is normal; if other bands show abnormal brightness differences, check gel preparation uniformity and electrophoresis conditions.

This product has pre-mixed universal ROX Reference Dye, compatible with all mainstream real-time PCR instruments, including ABI (e.g., 7500, 7900HT, StepOne, QuantStudio series), Bio-Rad (CFX96, iQ5), Roche (LightCycler series), Agilent, and other ROX-dependent and non-dependent instruments, without additional ROX or instrument setting adjustments. If you are unsure about instrument compatibility, contact technical support for confirmation.

No. The blue tracking dye is an inert dye that does not participate in the PCR amplification reaction, does not affect DNA polymerase activity, fluorescence signal acquisition, or amplification efficiency. Its sole purpose is to provide a visual cue during pipetting, helping identify sample positions and preventing missed, incorrect, or duplicate additions, particularly suitable for manual preparation of high-throughput reaction plates. Multiple brands have launched similar dye tracking products that have been fully validated.

Possible causes and solutions: Insufficient cycle number: check that the PCR program has sufficient cycles (40 cycles recommended) and confirm that fluorescence signal acquisition is enabled during the extension phase. Primer issues: confirm whether primers are degraded or improperly designed; consider re-synthesizing primers or optimizing primer design. Template issues: check whether template is degraded or concentration is too low; consider re-preparing template or increasing template input. Hot-start activation: confirm sufficient pre-denaturation/hot-start time (this product’s antibody-modified hot-start enzyme requires sufficient initial denaturation time to fully release enzyme activity).

High Ct values are typically related to low template amount, low amplification efficiency, or inhibitors in the system. Recommended solutions: Increase template input: appropriately increase the cDNA template proportion (not exceeding 20% of total volume). Optimize reverse transcription step: increase RNA input or switch to a more efficient reverse transcription kit. Optimize primer design: ensure amplicon length is 80-200 bp, avoiding high GC content or consecutive repeat structures. Eliminate PCR inhibitors: inhibitors carried in the template (such as ethanol, phenol) affect amplification efficiency; try gradient dilution of template or re-purify template. Check reaction conditions: confirm amplification efficiency is within 90%-110% via standard curve; if not, optimize annealing temperature or switch to a three-step protocol.

NTC amplification signals typically occur in two situations: Primer dimers (Ct > 35, Tm < 80 degrees C): non-specific amplification from primer dimers is quite common. Optimize primer design, reduce primer concentration (e.g., from 500 nM to 200-300 nM), or increase annealing temperature. Reagent or water contamination (Ct < 35): indicates the reaction system has been contaminated with exogenous DNA. Replace with fresh reagents and deionized water, prepare the reaction system in a laminar flow hood, and use filtered tips. Aerosol contamination: if large amounts of qPCR or cloning experiments have been previously performed in the lab, amplification product aerosol contamination may be present.

Double peaks in the melt curve typically indicate non-specific amplification or primer dimers. Solutions: Extra peak Tm 80 degrees C: likely non-specific product amplification. Use BLAST to check primer specificity, and redesign primers if issues persist. Also use NRC (No RT Control) to rule out gDNA contamination. Gel verification: if necessary, confirm PCR product size matches expectations via high-resolution agarose gel electrophoresis.

Poor reproducibility is typically related to pipetting errors or instrument status: Pipetting errors: regularly calibrate pipettes, use stable-performance pipettes. Consider enlarging the reaction volume (e.g., from 20 microL to 25-30 microL) or performing high-fold dilution of template and adding in larger volumes to reduce relative pipetting error. Preparing a master mix before dispensing into wells can also effectively reduce error. Instrument issues: regularly calibrate the real-time PCR instrument to ensure consistent temperature control across all wells. Template amount too low: random error increases at very low template concentrations; increase template amount to keep Ct values between 15-30.

This product has been buffer-optimized for high GC content templates, effectively improving amplification efficiency. If amplification difficulties persist, recommendations: Optimize annealing temperature: determine optimal annealing temperature through temperature gradient PCR; may need to decrease annealing temperature by 2-4 degrees C if necessary. Use three-step protocol: adding a temperature gradient between annealing and extension helps denature high GC templates. Add PCR enhancers: consider using DMSO or betaine to help open secondary structures in high GC regions. Design shorter amplicons: control amplicon length within 100-150 bp.

This product should be stored at -20 degrees C protected from light, minimizing repeated freeze-thaw. Aliquoting is recommended to reduce freeze-thaw cycles. Transport conditions: <0 degrees C (ice packs or dry ice). If slight precipitate appears after long-term storage at -20 degrees C, this is normal. After thawing at room temperature, invert to mix and it can be used normally. Do not vortex to avoid bubble formation.

YALI Biotech YALEPIC Universal Blue has significant advantages in multiple dimensions: Better cost-effectiveness: compared to Thermo Fisher Power SYBR Green Master Mix and Bio-Rad iTaq Universal SYBR Green Supermix and other imported brands, this product offers comparable performance at a more competitive price. Tracking design: the unique blue tracking system is superior to most imported products in pipetting accuracy, especially for high-throughput manual pipetting, effectively reducing operational error rates. Full platform compatibility: pre-mixed universal ROX, compared to some products that require users to add ROX or differentiate ROX models, this product is simpler to use. Local technical support: domestic production with faster technical support response, shorter supply cycles, and more convenient after-sales service. Core performance: based on antibody-based hot-start DNA polymerase and specially optimized buffer system, it effectively inhibits non-specific amplification, significantly improving amplification efficiency and detection sensitivity, suitable for low-copy template detection and high GC content template amplification.

The universality is reflected in two aspects. First, universal ROX premixed design – the product includes universal ROX Reference Dye, requiring no additional ROX adjustment for different qPCR instruments. It is directly compatible with StepOne, ABI 7500/7500 Fast, LightCycler 480, Bio-Rad CFX Connect/CFX96, and other mainstream real-time PCR systems. Second, sample universality – the optimized reaction system supports genomic DNA (gDNA), cDNA, plasmid DNA, and other template types, suitable for high GC content fragments and low-copy template amplification.

Three main advantages. First, antibody-modified hot-start DNA polymerase completely blocks enzyme activity at room temperature, effectively suppressing non-specific amplification and primer-dimer formation for higher data specificity. Second, the buffer system optimized for low-copy templates maintains good linearity in low-concentration template ranges, significantly improving detection sensitivity. Third, universal ROX premix is compatible with all instruments without additional ROX adjustment.

Both share the same core formulation with antibody-modified hot-start enzyme and universal ROX premix. The difference is that the Blue version adds a blue tracking dye for visual confirmation of pipetting and mixing, helping reduce pipetting errors – especially useful for high-throughput qPCR and multi-plate operations. Choose the colorless version for routine expression analysis and the Blue version for large-scale multi-well plate operations.

Yes, this is a 2x ready-to-use master mix containing SYBR Green I fluorescent dye, dNTPs, Mg2+, antibody-modified hot-start DNA polymerase, and universal ROX reference dye. Users only need to add template (cDNA or gDNA), forward and reverse primers, and nuclease-free water (ddH2O) to achieve a final MasterMix concentration of 1x for direct machine loading.

This is a two-step qPCR master mix and does not support one-step RT-qPCR. For one-step RT-qPCR (reverse transcription and qPCR in one tube), use YALEPIC U+ One Step qRT-PCR Probe MasterMix (CAT#YPQ42003). Alternatively, first reverse transcribe RNA using a YALI Biotech cDNA synthesis kit (sold separately), then use this product for quantitative amplification. The two-step strategy offers greater flexibility for complex samples or rare transcript quantification.

Store at -20 degrees C in the dark. Shelf life is 24 months; refer to the product label for exact expiry date. The product contains SYBR Green I fluorescent dye – strictly avoid light during storage and reaction setup. For frequent use (daily), short-term storage at 2-8 degrees C is acceptable; mix thoroughly before each use. Long-term storage at -80 degrees C is not recommended as some components may form precipitate.

The product is shipped with ice packs at below 0 degrees C. If ice packs melt completely and the product thaws, mix thoroughly and test functionality before use. Brief thawing (1-2 days) without repeated freeze-thaw typically does not affect performance. If completely thawed and re-frozen (one freeze-thaw cycle), use promptly to avoid activity degradation from repeated freeze-thaw.

The product has good stability at room temperature and does not require strict ice-bucket operation. The antibody-modified hot-start polymerase is inactive at room temperature, so reaction setup at room temperature will not significantly affect amplification specificity. However, if ambient temperature is high (>30 degrees C) or setup time is extended (e.g., >60 minutes for large-batch pipetting), place the master mix on ice or a cooling platform to reduce non-specific risk.

This high-sensitivity qPCR master mix is specifically optimized for low-copy templates. Functionally verified to maintain excellent linearity (R2 >=0.99) in low-concentration template ranges (cDNA input 1-10 ng, gDNA input 10-100 ng), with detection sensitivity for low-copy targets (10-100 copies/reaction) significantly superior to conventional qPCR reagents.

Specificity is ensured by two factors. First, the core component is antibody-modified hot-start DNA polymerase – the antibody binds to the enzyme at room temperature, blocking activity. Only at PCR pre-denaturation high temperature (typically 95 degrees C for 2-5 minutes) does the antibody dissociate and release polymerase activity, effectively suppressing non-specific primer extension at room temperature. Second, the buffer system optimized for SYBR Green dye chemistry minimizes primer-dimer formation.

Yes. The optimized buffer system effectively reduces secondary structure formation during annealing of high GC content fragments, significantly improving amplification efficiency and yield. Suitable for promoter regions, CpG islands, GC-rich gene coding regions, and other complex templates.

Yes. The premix system and universal ROX design make it ideal for high-throughput qPCR scenarios including 384-well plates, 96-well plates, and microfluidic chips. The Blue tracking version (YALEPIC Universal Blue SYBR Green qPCR MasterMix) further helps with pipetting quality control during high-throughput operations.

Common causes: (1) Template concentration too low or degraded – prepare fresh template, ensure cDNA purity and integrity; (2) Suboptimal primer design – ensure amplicon length 100-300 bp, primer Tm difference <2 degrees C, optimal Tm 60-65 degrees C; (3) Inaccurate pipetting – use calibrated pipettes, prepare pre-mix then dispense; (4) SYBR Green I dye exposed to light – ensure master mix is always stored and used in the dark.

Multiple or non-specific peaks (lower Tm peaks besides the main peak) typically indicate non-specific amplification or primer-dimers. Troubleshooting: (1) Check primer specificity, redesign if necessary; (2) Increase annealing temperature (e.g., from 60 to 62-65 degrees C); (3) Ensure adequate hot-start activation – 95 degrees C pre-denaturation for 2-5 minutes; (4) Check template purity for PCR inhibitors or cross-contamination.

NTC (no-template control) amplification indicates contamination or primer-dimers. Possible sources: (1) Reagents contaminated with nucleic acids – replace with fresh reagents and consumables, set up in a clean biosafety cabinet; (2) Primer-dimer non-specific amplification – optimize primer design or increase annealing temperature; (3) Laboratory aerosol contamination – regularly clean bench surfaces and pipettes, use filter tips and dedicated qPCR areas.

Poor reproducibility (CV typically required <5%, ideal <3%) causes and solutions: (1) Pipetting errors – use the pre-mix method: combine all MasterMix, primers, and water into a master mix, then dispense to individual wells; (2) Inconsistent template input – ensure thorough mixing of template before each pipetting; (3) Instrument well-to-well temperature variation – check thermal uniformity, avoid edge wells for critical data; (4) Air bubbles – flick or briefly centrifuge to remove bubbles after dispensing.

Possible causes and solutions: (1) PCR program parameter errors – verify fluorescence acquisition is set at the correct temperature step (SYBR Green typically during extension at 60-72 degrees C); (2) Reagent contamination or failure – replace with fresh MasterMix and primers; (3) Incorrect instrument fluorescence channel – verify channel matches SYBR Green I excitation/emission (excitation ~497 nm, emission ~520 nm); (4) Air bubbles or evaporation – ensure proper plate sealing, centrifuge to remove bubbles.

Yes. The product is optimized for fast thermal cycling and can be used with fast qPCR instruments (ABI 7500 Fast, StepOnePlus, QuantStudio series, etc.). Recommended fast program: 95 degrees C pre-denaturation/hot-start activation 3 minutes; 95 degrees C denaturation 1-5 seconds; 60 degrees C annealing/extension 30 seconds (fluorescence acquisition). Total cycle time can be further reduced by shortening denaturation and annealing/extension dwell times, but verify amplification efficiency and specificity.

Primer-dimer formation is the most common interference in SYBR Green dye chemistry. Improvement strategies: (1) Optimize primer design – avoid 3′ end complementarity, ensure amplicon 100-300 bp; (2) Reduce primer concentration – from 0.4 microM to 0.2 microM or lower; (3) Increase annealing temperature – increment by 1-2 degrees C until dimer signal significantly decreases; (4) Ensure adequate hot-start activation – 95 degrees C for 2-5 minutes; (5) Reduce cycle number – if sensitivity permits, reduce from 40 to 35-38 cycles.

You can obtain the COA (Certificate of Analysis) and technical support through: Visit the YALI Biotech website Product Center page, select the YALEPIC Universal SYBR Green qPCR MasterMix product page, and click the COA tab to download. For technical support, call 0512-66256010 or email info@yalibiotech.com.

Probe-based qPCR (such as TaqMan method) generates fluorescence signals through target-specific probe complementary binding to the target sequence, offering higher specificity and multiplex detection capability. It is suitable for SNP genotyping, allele identification, and other applications requiring high resolution, but has higher costs and more stringent primer/probe design requirements. Dye-based method (SYBR Green) detects signals through fluorescent dye binding to double-stranded DNA, offering low cost and strong versatility, but with relatively lower specificity and inability to distinguish non-specific amplification products. This product is a probe-based dedicated master mix, suitable for experimental scenarios requiring high specificity.

This product has a built-in dUTP/UDG contamination prevention system, with dUTP and uracil-DNA glycosylase (UDG) already included in the master mix. Before the qPCR reaction begins at room temperature, UDG selectively cleaves PCR contaminants (aerosols) containing dUTP, rendering them unable to serve as amplification templates. After PCR thermal cycling begins (typically >50 degrees C), the heat-labile UDG is irreversibly inactivated and does not affect subsequent amplification efficiency. Users do not need to add any additional anti-contamination components – ready to use.

This product is available in two versions: 50x ROX Reference Dye 1 and 50x ROX Reference Dye 2. Selection guidelines: ABI 7900HT/7300 Real-Time PCR System and StepOnePlus instruments use ROX Reference Dye 1; ABI 7500/7500 Fast/Stratagene Mx3000P instruments use ROX Reference Dye 2; Roche LightCycler series and Bio-Rad CFX series instruments that do not require ROX do not need it added. Different instrument models have different requirements for ROX reference fluorescence – please confirm your instrument model and select accordingly.

Yes. This product is based on an optimized buffer system and hot-start DNA polymerase, suitable for multiplex fluorescent probe qPCR detection, supporting at least four-plex simultaneous target detection. The product demonstrates good signal separation and consistent amplification performance across different fluorescence channels (FAM/VIC/CY5, etc.).

Yes. This product has undergone specific buffer optimization for low-copy templates. Combined with a new-generation hot-start DNA polymerase, it can detect fewer than 10 copies of nucleic acid template in a 20 microL system. It demonstrates excellent amplification efficiency and linearity in low-abundance target detection, suitable for rare transcript detection, trace pathogen detection, and other applications.

This product is recommended for long-term storage at -20 degrees C, and should be maintained below 0 degrees C during transport and short-term storage. Freeze-thaw stability testing has verified that the product maintains stable amplification performance after 50 repeated freeze-thaw cycles (Ct value deviation within +/-0.5). However, to avoid unnecessary performance fluctuations, aliquoting after first use is recommended to reduce repeated freeze-thaw cycles.

Abnormal amplification curves can typically be investigated from the following aspects: (1) Check template concentration – both too high and too low may cause abnormal curves; try gradient dilution and re-detection; (2) Confirm primer and probe design quality and concentration ratio; (3) Check for bubbles in the reaction system – centrifuge before loading to remove; (4) Confirm fluorescence acquisition step is correctly set – TaqMan probe method typically acquires signal at the end of annealing or extension, and must not be mixed with dye-based methods; (5) Rule out consumable and instrument cross-well interference. If problems persist, use positive controls to verify reagent performance.

Yes. This product is compatible with fast qPCR amplification protocols, enabling amplification detection within shortened cycle times to improve laboratory detection throughput. Under fast protocols, the product maintains good amplification efficiency (tested at >99%) and detection consistency.

Non-specific amplification and NTC signal are typically caused by: (1) Suboptimal primer design with dimers or hairpin structures; (2) Annealing temperature too low – try increasing annealing temperature (typically 2-5 degrees C increment); (3) Magnesium ion concentration too high – reduce appropriately or select a more compatible master mix system; (4) Aerosol contamination in the environment – the built-in dUTP/UDG contamination prevention system provides effective inhibition, but strengthening laboratory best practices is still recommended.

YALEPIC UR uses a dual-species antibody-based hot-start DNA polymerase with stricter low-temperature blocking, combined with an enhanced buffer optimized for low-copy templates, effectively inhibiting non-specific amplification and primer-dimer formation. Compared to conventional qPCR master mixes, this product is particularly suitable for accurate quantification of low-abundance target genes and amplification of high GC content complex templates, with outstanding performance in sensitivity and amplification specificity, and better data reproducibility.

Antibody-based hot-start DNA polymerase uses specific antibodies to bind Taq enzyme, blocking polymerase activity at low temperatures (e.g., room temperature to 55 degrees C). The antibody only denatures and releases active enzyme when the PCR reaction enters the high-temperature denaturation phase (typically >=90 degrees C). This mechanism significantly inhibits primer-dimer and non-specific amplification that may occur during the initial setup phase, markedly improving detection specificity and amplification efficiency. It is particularly suitable for low-copy template and high-sensitivity detection experiments, effectively enhancing data accuracy and reliability.

YALEPIC UR SYBR Green qPCR MasterMix is supplied with dual independent tubes of ROX Reference Dye. Users can add ROX according to their qPCR instrument model requirements – if the instrument requires ROX correction (such as ABI 7500, StepOnePlus, etc.), add the recommended amount; if the instrument does not require ROX correction (such as Bio-Rad series, etc.), add ddH2O directly. The dual independent ROX design ensures comprehensive compatibility across different instrument brands.

Yes. YALEPIC UR has undergone deep optimization of buffer system and enzyme formulation specifically for low-copy template detection, effectively improving amplification efficiency and detection sensitivity for low-abundance templates. It maintains good linearity and reproducibility over a dynamic range of 6 orders of magnitude. Even for low-expression samples with high Ct values, stable and reliable quantitative results can still be obtained, particularly suitable for clinical samples, rare cells, and viral load analysis in low-abundance detection scenarios.

For low-abundance target genes with high Ct values (Ct > 32), the following optimization strategies are recommended: (1) Select a high-sensitivity qPCR master mix, such as YALEPIC UR SYBR Green qPCR MasterMix; (2) Optimize primer design and reaction conditions to ensure primer specificity and amplification efficiency; (3) Appropriately increase template input; (4) Ensure regular calibration and temperature accuracy of qPCR instruments; (5) Use nuclease-free water and consumables to prevent exogenous nucleic acid contamination. This product, with the dual assurance of antibody-based hot-start enzyme and low-copy optimized buffer, can effectively improve detection sensitivity for low-abundance targets. Combined with the above strategies, more ideal quantitative data can be obtained.

YALEPIC UR SYBR Green qPCR MasterMix has undergone specific buffer formulation optimization for high GC content templates, effectively reducing amplification difficulties and abnormal melt curve peak shapes caused by high GC content. This product has been successfully applied to qPCR detection of various high GC content special genes and promoter regions, with single sharp melt curve peaks for amplification products. Amplification efficiency and specificity meet research requirements.

YALEPIC UR SYBR Green qPCR MasterMix uses a high-specificity antibody-based hot-start enzyme that effectively inhibits non-specific product formation, which is significant for SNP genotyping experiments that depend on amplification specificity. Combined with high-resolution melt curve analysis or allele-specific quantification methods, precise SNP identification and allele frequency analysis can be achieved.

For long-term storage, this product should be sealed and stored at -20 degrees C protected from light, with a shelf life of 24 months. After thawing, if short-term use is needed, it can be stably stored at 4 degrees C protected from light for 1 month. However, avoid repeated freeze-thaw (aliquoting is recommended to reduce freeze-thaw cycles). Because the product contains SYBR Green I fluorescent dye, storage and handling should be protected from light throughout to prevent fluorescence signal attenuation.

SYBR Green I is a double-stranded DNA intercalating fluorescent dye that emits minimal fluorescence when free in solution. Upon binding to PCR amplification product double-stranded DNA, the fluorescence signal increases hundreds-fold. The qPCR instrument achieves accurate quantification of target DNA by monitoring fluorescence intensity in real time for each cycle. This product has pre-mixed the optimal concentration of SYBR Green I. Before use, note: (1) Gently and thoroughly mix the MasterMix (vigorous vortexing produces bubbles leading to inaccurate reaction volumes); (2) Store and handle in the dark to prevent fluorescence signal attenuation; (3) Design high-specificity primers to reduce the impact of non-specific amplification and primer-dimers on detection results; (4) After the experiment, melt curve analysis is recommended to verify product specificity.

When amplification abnormalities occur, follow these troubleshooting steps: (1) Verify that the master mix is fully thawed and mixed (briefly centrifuge and gently pipette to mix); (2) Check primer design and concentration optimization (recommended primer length ~25 bp, Tm 60-65 degrees C, amplicon ~150 bp); (3) Check template quality and input amount; (4) Confirm qPCR instrument temperature calibration and inter-well consistency; (5) Use freshly prepared primers and nuclease-free water to rule out exogenous contamination. YALEPIC UR has undergone multiple formulation optimizations to effectively reduce non-specific background and improve amplification curve quality.

The UDG (Uracil-DNA Glycosylase) contamination prevention system replaces traditional dTTP with dUTP incorporated into DNA. Before PCR, heat-labile UDG enzyme hydrolyzes uracil-containing contaminating DNA at room temperature, preventing it from being amplified. During PCR thermal cycling, UDG is inactivated and does not affect subsequent reactions. This technology effectively prevents false positives caused by laboratory aerosol contamination, particularly important for clinical detection and high-sensitivity gene quantification research.

Antibody-modified hot-start enzymes physically block Taq enzyme active sites through antibodies at room temperature. Upon heating to hot-start temperature (typically >95 degrees C), the antigen-antibody complex denatures and releases enzyme activity. Compared to chemical modification, the antibody method offers faster activation, lower residual activity, and stronger inhibition of non-specific amplification, particularly suitable for high-sensitivity detection of low-copy templates.

High GC templates easily form secondary structures. This product’s buffer has been optimized for high GC systems. If amplification results are still unsatisfactory, recommendations: (1) Optimize annealing temperature (increase by 3-5 degrees C); (2) Add 5-10% DMSO or betaine as final concentration to assist strand separation; (3) Ensure complete template denaturation (extend initial denaturation at 95 degrees C to 5-10 minutes).

It is recommended to test each primer at a final concentration starting from 0.2 microM, with optimization in the 0.1-0.5 microM range. If non-specific amplification or primer-dimers occur (melt curve extra peaks), try reducing primer concentration to 0.1-0.15 microM. If amplification efficiency is low, test in a 0.2-0.4 microM gradient.

Check the following points: (1) Template concentration too high or too low (cDNA template recommended 1-10 ng/reaction, genomic DNA 10-100 ng/reaction); (2) Primer design should avoid consecutive GC at 3′ end; (3) Confirm ROX reference dye type matches instrument (this product’s universal ROX is compatible with all models); (4) Check that the amplification system is thoroughly mixed – SYBR Green I dye should not be vigorously shaken to produce bubbles; (5) Ensure precipitate is fully dissolved and mixed after reagent thawing.

This product may show white precipitate after low-temperature storage or freezing, which is a normal phenomenon of SYBR Green I dye and buffer salt precipitation. Place at room temperature protected from light for 3-5 minutes, gently invert to mix (do not vortex vigorously) until precipitate is fully dissolved. This does not affect reagent performance.

This product contains pre-mixed universal ROX reference dye, compatible with all mainstream qPCR instruments: ABI (7500/7500 Fast/ViiA7/QuantStudio series), Bio-Rad (CFX96/CFX Connect, etc.), Roche LightCycler series, Agilent Mx series, Jena, and other brands. No additional ROX purchase needed, greatly simplifying the experimental workflow.

This product is a SYBR Green dye-based qPCR master mix and does not contain reverse transcriptase components. For one-step qPCR, use with YALI Biotech reverse transcription kits, or select YALI Biotech one-step qPCR master mix product series. YALEPIC U+ One Step qRT-PCR Probe MasterMix CAT#YPQ42003.

For gene expression differential analysis, primer screening, and other rapid quantification, this SYBR Green method (universal high-sensitivity) is recommended. For multiplex detection, absolute quantification, or clinical-grade high-specificity detection, YALI Biotech probe-based qPCR master mix product series CAT#YQ32032 is recommended.

YALI Biotech offers trial samples for the full range of YALEPIC qPCR master mix products for new customers. You can apply through the official customer service hotline (0512-66256010) or email info@yalibiotech.com. Submit your research direction and estimated usage to receive free samples.

Yes. The optimized reaction buffer system effectively enhances amplification of high GC content templates. The product has good tolerance for complex templates and samples containing inhibitors.

Troubleshooting steps: Check template quality – verify DNA is not degraded or impure by electrophoresis, use high-quality template from kit extraction. Check primer design – ensure no secondary structures or dimers, recommended length 22-35 mer, Tm >60 degrees C, GC 45-60%. For long fragments, primer length 25-35 mer, Tm >=65 degrees C. Check reaction setup – ensure all components added and mixed; reagents fully dissolved. Optimize annealing temperature – try gradient 55-68 degrees C. Increase cycles by 5-10. Check thermal cycler – verify program, lid and block temperatures.

Non-specific amplification causes and solutions: Low annealing temperature – increase appropriately or use Touchdown PCR. Poor primer specificity – optimize design, BLAST verify. High primer concentration – reduce to 0.2-0.5 microM. Excessive or impure template – reduce amount, use high-quality purified template. Too many cycles – reduce to 25-30 cycles.

YALEPIC NovaAlign Ultra HiFi MasterMix produces blunt-ended PCR products because the enzyme has 3′ to 5′ exonuclease (proofreading) activity and does not add A overhangs like Taq polymerase. Blunt-end products can be used directly with blunt-end cloning vectors. For TA cloning, perform A-tailing on the PCR product before ligation with T-vector.

Dye-free version (YEM51008-A): no electrophoresis indicator dye, suitable for PCR products requiring further purification (digestion, ligation, sequencing). Dye-containing version (YEM51008-B): pre-mixed with electrophoresis tracking dye, allowing direct gel loading after PCR without additional Loading Buffer, simplifying workflow for high-throughput screening and rapid detection. Choose dye-free for downstream experiments (cloning, sequencing) and dye-containing for rapid PCR product detection.

Storage: -20 degrees C long-term. Transport: below 0 degrees C (dry ice or ice packs). Avoid repeated freeze-thaw; tested for 30 freeze-thaw cycles without significant performance impact. For frequent short-term use, store at 2-8 degrees C. Aliquot after first use to minimize freeze-thaw cycles.

YALEPIC NovaAlign Ultra HiFi MasterMix has good tolerance to PCR inhibitors. Compared to regular Taq, it has stronger tolerance to common blood PCR inhibitors and can directly use crude sample lysate as template, simplifying sample pre-processing. For samples with many inhibitors, reduce sample input or perform simple dilution.

The product contains a low-concentration inert electrophoresis tracking dye. The formulation has been optimized to not interfere with Taq enzyme activity, primer annealing, or DNA amplification. It does not inhibit the PCR reaction at any point and does not affect band clarity.

It can be used directly for colony PCR. The modified Taq enzyme tolerates bacterial impurities. Pick a single colony and add it directly to the reaction system for amplification. In standard scenarios, no pre-lysis of bacteria is needed.

Yes. The amplification products of this master mix naturally have 3′ A-overhangs. After purification, the products can be directly used for T/A cloning ligation without terminal A-addition enzyme treatment.

Aliquoting into single-use portions is recommended, stored at -20 degrees C. After up to 20 freeze-thaw cycles, enzyme activity shows no significant decline. Long-term storage at 4 degrees C is not recommended; short-term (within 3 days) temporary storage is acceptable.

The optimized buffer system enhances impurity tolerance. Crude tissue extracts and simply extracted nucleic acids can be stably amplified. If impurity levels are excessive, appropriately dilute the template concentration.

All specifications in the YEM61001 series have identical formulations, with only different aliquot volumes. Products from the same batch can be freely mixed for reaction system preparation.

25 microL system: 12.5 microL MasterMix + 0.2-0.5 microL each of forward and reverse primers + appropriate template + RNase-free water to 25 microL.

(1) Primer degradation or poor design specificity; (2) Template degradation or concentration too low; (3) Annealing temperature mismatch – set up a temperature gradient to optimize conditions; (4) Reagent degradation from improper storage – test with a new batch of master mix.

Minor color variation between batches is normal and does not affect amplification or electrophoresis results. All products pass COA quality inspection before release.

Appropriately increase template input, fine-tune annealing temperature. If cDNA purity is low, dilute the template to reduce interference from reverse transcription residual inhibitors.

Brief room temperature exposure (<12 hours) generally does not cause loss of effectiveness. After aliquoting, store at -20 degrees C protected from light. A small-scale PCR test can be performed to verify amplification performance before large-scale use.

Compatible with both standard and fast PCR protocols. Short fragments can use shortened extension times, combined with fast temperature control programs to increase experimental speed.

The fidelity of YALEPIC Novalign Flash HiFi MasterMix is approximately 108 times that of regular Taq DNA polymerase. Its DNA polymerase has 3′ to 5′ exonuclease (proofreading) activity, which can real-time identify and excise mismatched nucleotides during amplification, ensuring extremely high replication accuracy. This characteristic makes it particularly suitable for gene cloning, site-directed mutagenesis analysis, NGS library construction, and other applications with stringent sequence fidelity requirements.

The extension speed of this product has two tiers based on target fragment length: for fragments <=3 kb: extension speed up to 1 sec/kb; for fragments <=10 kb: extension speed 5 sec/kb. Compared to conventional high-fidelity polymerases at 15-30 sec/kb, this product can significantly shorten PCR reaction time, enabling fast PCR. For difficult long fragment amplification, appropriately extending extension time based on actual conditions is recommended to ensure yield.

This product is available in two versions: YEM51006-A (non-Dye, standard) – does not contain tracking dye, suitable for applications requiring fluorescence signal detection (such as qPCR, NGS library amplification); YEM51006-B (Dye version) – contains electrophoresis tracking dye, allowing direct agarose gel electrophoresis after PCR, simplifying operation and saving time.

Yes. This product’s formulation has been optimized with strong complex template compatibility, capable of directly amplifying high GC content fragments within a certain range. For extremely high GC content difficult templates, the following optimization strategies are recommended: (1) Appropriately increase denaturation temperature or extend denaturation time; (2) Use gradient annealing temperature to test optimal conditions. For further optimization, contact YALI Biotech technical support for professional guidance.

YALEPIC Novalign Flash HiFi MasterMix produces blunt-ended amplification products, because its DNA polymerase has 3′ to 5′ proofreading activity and does not add extra A bases at the 3′ end of products. Direct use of blunt-end cloning vectors for ligation is recommended for highest efficiency. If TA cloning is necessary, first purify the PCR product to completely remove the high-fidelity polymerase, then use Taq DNA polymerase to add A-tails to the 3′ end of the purified blunt-end products, followed by TA ligation.

Possible causes and solutions: Low annealing temperature – increase annealing temperature; gradient annealing temperature optimization recommended (typically test 50-65 degrees C range). Poor primer design – redesign primers, ensure 3′ end specificity, avoid self-complementary primers. Primer concentration too high – reduce primer concentration (0.2-0.5 microM final concentration recommended). Inappropriate template amount – reduce template usage; 50-200 ng genomic DNA is typically appropriate. Too many cycles – reduce cycle number; 25-35 cycles typically sufficient for most amplification needs. If smearing persists, use freshly extracted high-quality template DNA and check the storage condition of all reagents.

No. The MasterMix may show small amounts of precipitate after low-temperature storage or repeated freeze-thaw, which is a normal phenomenon. Before use, remove the MasterMix and thaw at room temperature, gently inverting to mix every 10-15 minutes until the precipitate is fully dissolved, then briefly centrifuge to collect. Performance testing shows that multiple freeze-thaw cycles (up to 30) have no significant effect on PCR amplification performance. For long-term storage, aliquot after first use to reduce repeated freeze-thaw.

Follow these best practices to minimize PCR contamination risk: Spatial separation: designate a dedicated PCR setup area in the lab, strictly separated from template handling and PCR product analysis areas. Dedicated equipment: equip the setup area with dedicated pipettes, disposable filtered tips, and PCR tubes; avoid cross-use. Proper technique: briefly centrifuge PCR reagent tubes before opening to settle liquid to the bottom, reducing contamination of gloves and pipettes. Template management: dilute DNA templates at your own bench, bringing only the required dilutions into the setup area. Product isolation: never bring PCR tubes containing amplification products into the setup area after PCR; perform subsequent electrophoresis detection in designated areas. Set up controls: set up no-template negative controls (NTC) for every PCR reaction to monitor for DNA contamination.

The fidelity of this product is approximately 108 times that of regular Taq DNA polymerase. The high fidelity comes from the polymerase’s strong 3′ to 5′ exonuclease activity (i.e., proofreading function), which can excise incorrectly incorporated nucleotides, ensuring highly accurate amplified sequences with an error rate far lower than conventional Taq enzyme.

The DNA polymerase used in this product has been genetically engineered with greatly enhanced processivity. The extension speed can reach 10 sec/kb, meaning a 10 kb fragment can be extended in 100 seconds, significantly faster than traditional high-fidelity enzymes (which typically require 30-60 sec/kb).

This product is available in two versions: YEM51007-A (non-Dye, standard) – does not contain tracking dye, suitable for applications requiring fluorescence signal detection (such as qPCR, NGS library amplification); YEM51007-B (Dye version) – contains electrophoresis tracking dye, allowing direct agarose gel electrophoresis after PCR, simplifying operation and saving time.

Yes. This product’s formulation has been optimized with strong complex template compatibility, capable of directly amplifying high GC content fragments within a certain range. For extremely high GC content difficult templates, the following optimization strategies are recommended: (1) Appropriately increase denaturation temperature or extend denaturation time; (2) Use gradient annealing temperature to test optimal conditions. For further optimization, contact YALI Biotech technical support for professional guidance.

This product can efficiently amplify genomic fragments up to 40 kb. For long fragment PCR (especially fragments >10 kb), the keys to success are: Template quality: using high-purity, intact genomic DNA is critical. Avoid template degradation or inhibitors. Primer design: for amplicons exceeding 20 kb, primer GC content should be above 50%, Tm above 60 degrees C, and length at least 24 nucleotides. Experimental technique: handle gently when preparing the reaction system to avoid physical shearing of long-chain DNA templates.

Possible causes and solutions: Low annealing temperature – increase annealing temperature; gradient annealing temperature optimization recommended (typically test 50-65 degrees C range). Poor primer design – redesign primers, ensure 3′ end specificity, avoid self-complementary primers. Primer concentration too high – reduce primer concentration (0.2-0.5 microM final concentration recommended). Inappropriate template amount – reduce template usage; 50-200 ng genomic DNA is typically appropriate. Too many cycles – reduce cycle number; 25-35 cycles typically sufficient for most amplification needs. If smearing persists, use freshly extracted high-quality template DNA and check the storage condition of all reagents.

No. The MasterMix may show small amounts of precipitate after low-temperature storage or repeated freeze-thaw, which is a normal phenomenon. Before use, remove the MasterMix and thaw at room temperature, gently inverting to mix every 10-15 minutes until the precipitate is fully dissolved, then briefly centrifuge to collect. Performance testing shows that multiple freeze-thaw cycles (up to 30) have no significant effect on PCR amplification performance. For long-term storage, aliquot after first use to reduce repeated freeze-thaw.

Follow these best practices to minimize PCR contamination risk: Spatial separation: designate a dedicated PCR setup area in the lab, strictly separated from template handling and PCR product analysis areas. Dedicated equipment: equip the setup area with dedicated pipettes, disposable filtered tips, and PCR tubes; avoid cross-use. Proper technique: briefly centrifuge PCR reagent tubes before opening to settle liquid to the bottom, reducing contamination of gloves and pipettes. Template management: dilute DNA templates at your own bench, bringing only the required dilutions into the setup area. Product isolation: never bring PCR tubes containing amplification products into the setup area after PCR; perform subsequent electrophoresis detection in designated areas. Set up controls: set up no-template negative controls (NTC) for every PCR reaction to monitor for DNA contamination.

Possible causes and solutions: Primer design errors – homology arms should be 15-25 bp with GC content controlled at 40%-60%. If GC content is above 70% or below 30%, recombination efficiency will be significantly affected. Redesign primers accordingly. Low competent cell efficiency – use freshly prepared or properly stored competent cells with transformation efficiency >10^8 cfu/microgram. The ligation product volume should not exceed 1/10 of the competent cell volume. Insufficient DNA input or poor ratio – the optimal molar ratio of vector to insert is 1:2. Recommended formula: vector amount (ng) = 0.02 x vector base number; insert amount (ng) = 0.04 x insert base number. DNA purity issues – if using unpurified DNA, total volume should not exceed 1/5 of the reaction system (e.g., no more than 4 microL in a 20 microL system). Gel purification is recommended, with products dissolved in ddH2O. Kit issues – first use the positive control included with the kit to rule out kit quality problems.

This is typically not a kit issue but rather mutations introduced during PCR amplification. Possible causes: PCR-introduced mutations – use high-fidelity DNA polymerase for target fragment amplification instead of regular Taq. Non-specific PCR product contamination – check PCR product specificity by gel electrophoresis. Multiple PCR products will lead to incorrect inserts; gel purify PCR products before recombination. Incomplete vector linearization – even trace amounts of incompletely digested circular vector will produce high transformation background. Use a negative control to verify complete linearization and optimize digestion conditions.

Design principles: Homology arm length: 18-25 bp is ideal. Below 15 bp, recombination efficiency decreases significantly. GC content: 40%-60%. Above 70% or below 30% will affect efficiency. Sequence specificity: avoid repetitive sequences within 50 bp upstream/downstream of the cloning site. Primer design formula: 5′-[homology sequence (15-25 bp)]-[restriction site (optional)]-[gene-specific amplification primer]-3′. Note: this kit requires 15-25 bp of identical sequence between vector and insert ends for homologous recombination.

In short: homologous recombination is the method, seamless cloning is the result. Homologous recombination is a next-generation directional cloning technology that does not rely on restriction digestion and ligation steps. It uses 15-20 nt homologous sequences at the ends of DNA fragments and linearized vectors to achieve recombination, allowing fragments to be cloned at any site with very low vector self-ligation background.

Possible causes and solutions: Incomplete vector linearization – even trace circular vector produces high background. Solutions: increase restriction enzyme amount, extend digestion time, gel purify digested products, and use a negative control. Circular plasmid contamination with same resistance – when using circular plasmid as PCR template, residual plasmid produces background. Solutions: use pre-linearized plasmid as template, DpnI digest the PCR product, or gel purify. Insufficient plate antibiotics – ensure correct antibiotics and freshly prepared plates.

Recommended reaction times: 1-2 fragments: 5-15 minutes; 3-5 fragments: 15-30 minutes; vector >10 kb or fragment >4 kb: extend to 30-60 minutes. Strictly control the reaction temperature at 50 degrees C. Temperature deviations affect recombinase activity. If reaction mixture adheres to tube walls, briefly centrifuge to collect at the bottom.

Yes, but with limitations: If linearized vector and insert are already purified with single bands on gel, use a spectrophotometer for concentration measurement (A260/A280 between 1.8-2.0). For unpurified DNA, total volume should not exceed 1/5 of the reaction system. Note: EDTA and other metal ion chelators inhibit seamless cloning. Dissolve purified products in ddH2O, not Tris-EDTA buffer. Gel purification is recommended for best recombination efficiency.

Electrocompetent cells are not recommended. Use high-quality chemically competent cells (transformation efficiency >10^8 cfu/microgram). The recombination product should not exceed 1/10 of the competent cell volume to avoid transformation inhibition.

Recommended verification methods: Positive control – use the kit’s included positive control for parallel experiments. If the positive control produces colonies that test positive, kit issues can be ruled out. Colony PCR – use vector universal primers or at least one universal primer. Plasmid double digestion – extract plasmid from positive colonies and perform double restriction digestion at expected sites. Sequencing – final positive clones should be sequenced to confirm correct insert sequence.

This product can efficiently assemble 1-11 DNA fragments. For multi-fragment assembly, start with fewer fragments (2-3) to optimize conditions before gradually increasing.

Storage: -20 degrees C. Transport: below 0 degrees C. Minimize repeated freeze-thaw cycles. Thaw completely and mix gently before each use, return to -20 degrees C immediately after use. Use nuclease-free tips and tubes. The product tolerates up to 30 freeze-thaw cycles without significant performance loss. If slightly viscous upon thawing, ice-thaw to a clear liquid – this does not affect normal use.

Possible cause: unsuccessful recombination due to incomplete vector linearization. Solutions: verify complete linearization of the vector, optimize digestion conditions (increase enzyme amount, extend digestion time), gel purify digestion products to remove residual circular plasmid, and use validated vector universal primers for colony PCR.

It supports single living cells, embryo biopsy TE cells, trace amounts of peripheral blood, 10 pg-ng level trace genomic DNA, small amounts of FFPE dissociated single cells. A conventional single cell can stably amplify 2-5 microgram of genomic DNA.

The standard protocol completes all amplification in 3 hours: conventional lysis 30 min + pre-amplification + formal amplification. It is not recommended to arbitrarily shorten the reaction time, as this will reduce genome coverage.

Samples after cell lysis completion can be transported at room temperature at temperatures <=37 degrees C for short distances without compromising DNA integrity. This is a core advantage distinguishing this kit from conventional WGA products on the market. For long-distance transport, refrigeration is still recommended.

The product purity meets NGS library preparation requirements and can be directly used for whole-genome sequencing, exon capture sequencing, CNV copy number variation detection, SNP genotyping, and other downstream experiments.

It uses a directed-evolution optimized Phi29 polymerase with high fidelity and strong strand displacement activity, achieving lower amplification bias and whole-genome coverage >=95%, superior to conventional wild-type Phi29 materials.

The complete set includes lysis buffer, pre-amplification buffer, Phi29 enzyme, amplification buffer, and other full reaction reagents. No additional purchase of配套 components is needed – ready for use directly on the instrument.

Stock solutions must be stored at -20 degrees C protected from light. Repeated freeze-thaw or prolonged room temperature storage will cause enzyme activity degradation, resulting in low amplification yield and reduced coverage.

Amplification products should be aliquoted and stored at -20 degrees C for short-term (1 month) or at -80 degrees C for long-term (2 years). Repeated freeze-thaw should be <=3 times.

Usually caused by consumable or workbench exogenous DNA contamination. Replace with sterile, nuclease-free centrifuge tubes, UV-sterilize the workbench, and open a fresh negative control buffer to eliminate contamination.

Insufficient cell lysis, starting cell degradation, or reaction temperature fluctuation. Optimize lysis incubation time and add enzymes on ice throughout the process to improve product yield.

The optimized buffer is compatible with animal/human single cells. Plant cell walls are thick and require pre-optimization of the lysis protocol. A small number of plant samples can be validated through pilot experiments.

The reagent performance has been validated with PGT samples and can be used for blastocyst biopsy single-cell amplification and embryo chromosome CNV screening.

Core advantage comparison: Enzyme type – YALEPIC uses directed-evolution Phi29 enzyme with superior uniformity; conventional kits use wild-type Phi29 with higher bias. Coverage – YALEPIC >=95%; conventional typically 80%-90%. Amplification time – YALEPIC 3 hours; conventional 4-8 hours. Transport method – YALEPIC allows room temperature transport after lysis at <=37 degrees C; conventional typically requires full -20 degrees C cold chain. Product yield – YALEPIC 2-5 microgram; conventional 1-3 microgram. Application scenarios – YALEPIC supports WGS/WES/PGT/CNV/qPCR, etc.; conventional mostly limited to qPCR/genotyping. The YALEPIC single-cell WGA kit is a new-generation single-cell WGA solution that balances high coverage, high uniformity, operational convenience, and transport flexibility.

This kit uses a fluorescent dye method that specifically binds dsDNA, offering three major advantages over UV spectrophotometry: (1) High selectivity – detects only dsDNA, unaffected by RNA, free nucleotides, proteins, and other impurities; (2) High sensitivity – can detect dsDNA samples as low as 100 pg/microL, superior to the detection limit of UV methods; (3) Wide dynamic range – maintains good linearity in the range of 100 pg/microL to 100 ng/microL, enabling accurate quantification without multiple dilutions.

Yes. This kit is perfectly compatible with the full range of Qubit fluorometers, including Qubit 1.0, Qubit 2.0, Qubit 3.0, and Qubit 4.0. It is also compatible with fluorescence microplate readers from various brands equipped with fluorescence detection modules. When using Qubit, please select the 1x dsDNA High Sensitivity mode in the detection program for instrument calibration and sample measurement.

This kit provides two standards: Standard S1 (0 ng/microL) and Standard S2 (10 ng/microL). Correct usage steps: (1) Before use, invert gently to mix and briefly centrifuge for a few seconds – do not vortex; (2) Pipette 190 microL of working solution into Qubit assay tubes; (3) Add 10 microL of Standard S1 and Standard S2 to the respective tubes; (4) Vortex briefly for 2-3 seconds, briefly centrifuge, incubate in the dark for 2 minutes, then proceed with instrument calibration. Each time you use the Qubit fluorometer for detection, you must recalibrate using the standards provided with the kit.

This kit has good tolerance for the following common contaminants: (1) Salts – NaCl, KCl, etc.; (2) Proteins – BSA, enzymes, etc.; (3) RNA – total cellular RNA, rRNA, etc.; (4) Free nucleotides – dNTPs, NTPs, etc.; (5) Detergents – Triton X-100, Tween-20, SDS, etc.; (6) Organic solvents – ethanol, DMSO, etc. This feature allows users to directly quantify PCR amplification products and unpurified DNA samples without additional purification steps.

The complete kit should be stored at 2-8 degrees C, protected from light. After opening: (1) Working Solution (pre-mixed working solution) should be stored at 2-8 degrees C protected from light, avoiding repeated freeze-thaw; (2) Standard S1 and Standard S2 should be stored at 2-8 degrees C protected from light; (3) Fluorescent dyes are subject to quenching – all components should be handled in the dark before and after use, and caps should be tightened immediately and returned to the refrigerator; (4) Please complete sample detection within the specified time to avoid result deviation due to fluorescence quenching.

The final volume of each reaction in this kit is 200 microL, with working solution volume of 180-199 microL and sample volume of 1-20 microL. The recommended sample volume is 1-10 microL, which can be flexibly adjusted based on the estimated sample concentration. Please calibrate pipettes before use to ensure accurate sampling. After detection, calculate the original sample concentration using the following formula: (instrument reading concentration) x (working solution volume + sample volume) / sample volume.

The YALEPIC 1x dsDNA HS Assay Kit is highly comparable to the Thermo Fisher Qubit dsDNA HS Kit in core performance indicators: both maintain good linearity (R-squared > 0.99) in the 0.1-100 ng dsDNA range; high selectivity for dsDNA with minimal RNA interference; and comparable tolerance to common contaminants such as salts, proteins, and detergents. This kit offers a 500-assay specification with a more advantageous per-test cost, making it a high cost-effectiveness alternative to the Qubit dsDNA HS Kit.

Fluorescent dyes have natural quenching, and fluorescence intensity will slowly decay as incubation time increases. For stable and reliable results, it is recommended to: (1) After adding standards and samples, uniformly incubate in the dark for 2 minutes, then complete all detection within 30 minutes; (2) Avoid excessive temperature differences between samples and standards; (3) Do not expose to strong light for prolonged periods during detection. Following these conditions strictly, inter-batch and intra-batch detection results show good stability and reproducibility.

This kit is suitable for quantifying double-stranded DNA samples from various sources, including but not limited to: (1) NGS libraries – input DNA library quantification for high-throughput sequencing; (2) PCR/qPCR products – dsDNA quantification of amplification products; (3) Genomic DNA – gDNA extracted from cells, tissues, blood, and other samples; (4) Plasmid DNA – plasmid extraction products and restriction digest products; (5) cDNA – cDNA products from reverse transcription; (6) DNA standards – various types of dsDNA standards. The kit has no selectivity for the source species (human, animal, plant, microorganism, virus, etc.) and is universally applicable.

Standard errors or negative fluorescence values typically have the following causes and solutions: (1) Insufficient standard preparation – not fully mixed before use, please invert and briefly centrifuge; (2) Temperature effects – the assay is temperature-sensitive, ensure standards and samples are equilibrated to room temperature before detection; (3) Pipetting errors – use calibrated pipettes; (4) Bubble interference – briefly centrifuge after mixing to remove bubbles; (5) Standard deterioration – check whether standards are within their shelf life and whether they have undergone repeated freeze-thaw or contamination. If the problem persists, check the raw fluorescence values (RFU) of the standards and confirm that the sample fluorescence values fall between those of the standards.

This kit has a minimum detection limit of 1 pg/microL, with a linear range covering 1 pg/microL to 100 ng/microL, spanning 5 orders of magnitude. It is ideal for precise quantitative detection of ultra-low concentration RNA samples such as plasma cfRNA, serum cell-free RNA, urine RNA, exosome RNA, viral RNA (such as SARS-CoV-2, HBV, and other low-titer samples).

Qubit and NanoDrop are suitable for medium-to-high concentration RNA samples (typically >1 ng/microL), whereas liquid biopsy cfRNA, exosome RNA, and other samples often have concentrations below 1 ng/microL, which conventional methods cannot accurately detect. YALEPIC RNA HS uses a patented fluorescence-based quantification technology, specifically designed for pg-level ultra-low concentration RNA. It is a key quality control tool for ensuring accurate RNA input before NGS library construction, effectively preventing sequencing data bias caused by RNA quantification errors.

This kit is compatible with all mainstream real-time quantitative PCR instruments (such as ABI, Bio-Rad, Roche, Agilent, etc.), requiring no specialized equipment. The reagent is a pre-mixed formulation, ready to use out of the box with no additional preparation needed. From sample addition to results takes only about 30 minutes, with simple operation suitable for high-throughput laboratory batch testing.

The 1000 rxns specification is suitable for daily use in medium-to-large NGS laboratories. At one test per sample, 1000 rxns can test 1000 samples. The list price is 2925 CNY, making the cost per test only about 2.9 CNY. Bulk purchasing offers even more favorable pricing, providing excellent cost-effectiveness.

Absolutely. The ultra-high sensitivity (1 pg/microL) of this kit makes it ideal for precise quantification of viral RNA loads, including SARS-CoV-2, influenza virus, HBV, HCV, HIV, and more. Compared to traditional RT-qPCR quantification methods, this kit can first perform precise concentration measurement of extracted viral RNA, then guide the RNA input for subsequent qPCR reactions, significantly improving the accuracy and reproducibility of viral load detection.

Please store at -20 degrees C protected from light, avoid repeated freeze-thaw cycles, and use within 6 months after opening. For transport, -20 degrees C dry ice shipping is recommended. If shipping at ambient temperature, use ice packs for insulation and deliver within 72 hours. Please refer to the accompanying COA report for specific details.

Three core advantages: (1) Higher sensitivity – minimum 1 pg/microL, industry-leading; (2) Wider linear range – 5 orders of magnitude, covering trace to high concentrations in a single tube; (3) Better cost-effectiveness – 1000 rxns bulk packaging, with per-test cost as low as 2.9 CNY. Additionally, YALI Biotech provides comprehensive technical support and customized services, making it a premium choice for domestic high-throughput sequencing quality control reagents.

For bulk purchases, please call 0512-66256010 to contact the YALI Biotech sales team directly. YALI Biotech provides professional pre-sales technical consultation and post-sales application support, and can offer customized solutions based on your experimental needs.

The YALEPIC dsDNA HS Assay Kit uses a fluorescent dye binding method with the following core advantages: (1) High sensitivity – detection limit as low as 100 pg/microL, far below the detection limit of UV methods (approximately 2-5 ng/microL); (2) High specificity – binds only double-stranded DNA, unaffected by RNA, free nucleotides, proteins, etc., providing more accurate quantification in samples with severe RNA contamination or DNA degradation; (3) Contaminant tolerance – tolerates salts, detergents, proteins, and other common contaminants, suitable for direct quantification of complex samples.

Yes. The YALEPIC dsDNA HS Assay Kit is specifically designed for high-sensitivity dsDNA quantification, with the same principle and comparable performance as the Qubit dsDNA HS Assay Kit. It is fully compatible with Qubit fluorometers (including Qubit 3.0, Qubit 4.0, Qubit Flex) and can effectively replace imported brands for NGS library quantification during library construction. It is also compatible with fluorescence microplate readers for high-throughput sample detection. This product has been adopted in daily production by multiple domestic NGS testing institutions.

The minimum detectable concentration of this kit for dsDNA samples is 100 pg/microL. The actual minimum detectable total DNA amount depends on the volume of sample added. Standard protocol recommends adding 1-20 microL of sample to a 200 microL detection system, so theoretically the minimum detectable total dsDNA is approximately 0.1 ng. If the sample concentration is below the detection limit, concentrate the sample using vacuum concentration or ethanol precipitation before detection.

The YALEPIC dsDNA HS Assay Kit has good contaminant tolerance: it can tolerate up to 100 mM NaCl, 100 microgram/mL BSA, 0.1% Triton X-100, 5% DMSO, and 10 microM free nucleotides. However, high concentrations of detergents (such as SDS above 0.5%) or organic solvents may slightly inhibit fluorescence signal. For samples with high contaminant levels, dilute the sample to within the contaminant tolerance range before quantification, or purify using a DNA purification column first.

The kit provides two pre-diluted dsDNA standards (Standard #1 and Standard #2). On Qubit fluorometers, a two-point standard curve can be automatically established through the built-in program. It is recommended to create a new standard curve for every experiment, as fluorescence signals may fluctuate due to working solution preparation accuracy, ambient temperature, and other factors. When using a microplate reader, prepare serially diluted standards and fit a standard curve (four-parameter or linear regression equation) manually.

Recommended troubleshooting steps: (1) Confirm the working solution is correctly prepared and the fluorescence reagent volume is accurate; (2) Check the raw fluorescence values (RFU) of the standards and confirm that sample fluorescence values fall within the standard fluorescence range; (3) The assay is temperature-sensitive – fluorescence signal may decrease at high ambient temperatures, ensure samples and standards are equilibrated to room temperature before detection; (4) Avoid using working solution that has been repeatedly freeze-thawed; (5) Confirm that the detection parameter settings match the instrument model.

This kit is highly selective for double-stranded DNA, with the fluorescent dye specifically binding dsDNA. Cross-reactivity signal in the presence of RNA is less than 1%, and cross-reactivity signal in the presence of ssDNA is less than 2%. Therefore, this product is dedicated to precise quantification of dsDNA and is not suitable for ssDNA or RNA quantification. For total nucleic acid or RNA quantification, please select the corresponding YALI Biotech quantification kit.

The kit has a shelf life of 12 months when stored at 2-8 degrees C protected from light (from the date of manufacture). The YALEPIC dsDNA HS Reagent component is sensitive to light and must be stored in the dark. Do not freeze the kit or any of its components. The working solution should be used within 4 hours after dilution. For short-term storage (up to 1 week), it can be stored at 2-8 degrees C protected from light, though fluorescence signal may attenuate.

Yes. DNA extracted from FFPE samples typically has varying degrees of degradation and may contain PCR inhibitor residues. The YALEPIC dsDNA HS Assay Kit has good fluorescence response to degraded DNA fragments and can tolerate inhibitors that may remain in FFPE samples (such as xylene, ethanol, etc.). For highly degraded FFPE-DNA, it is recommended to dilute the standards to a fragment size range similar to the sample for more accurate quantification results.

The YALEPIC dsDNA HS Assay Kit is compatible with the following fluorescence detection instruments: (1) Qubit series fluorometers: Qubit 3.0, Qubit 4.0, Qubit Flex, Qubit 2.0; (2) Fluorescence microplate readers: BioTek Synergy series, Molecular Devices SpectraMax series, Tecan Infinite series, BMG CLARIOstar series, and other microplate readers equipped with fluorescence detection modules (excitation wavelength approximately 480-500 nm, emission wavelength approximately 520-540 nm). Please confirm that the instrument fluorescence channel settings match before use.

The kit stably recovers DNA fragments as small as 100 bp. By adjusting the bead-to-sample ratio, it can size-select up to 10 kb large genomic DNA fragments, accommodating both small NGS library fragments and large gDNA purification.

The 5 ml size (YNQ46001-01) is suitable for small-scale, manual NGS library preparation in research labs. The 60 ml size (YNQ46001-03) is designed for high-throughput laboratories and automated workstations for batch sample processing.

Yes. The selection principle, operating ratios, and fragment size selection range are consistent with AMPure XP. The purification recovery rate is comparable to imported products while significantly reducing NGS library preparation reagent costs.

Freezing is strictly prohibited. Freezing damages the surface functional groups of the beads, resulting in loss of DNA binding capacity. Once frozen, the kit must be discarded. Store at 2-8 degrees C.

Four common causes: (1) Beads not equilibrated to room temperature before use; (2) Insufficient mixing of beads and sample; (3) Incorrect bead addition ratio; (4) Over-drying of beads. Optimizing these steps per the protocol will improve recovery.

Fully compatible. The buffer formulation is optimized for automated platforms. Mainstream high-throughput workstations can use it directly without additional formulation adjustments.

Appropriately reduce the bead-to-sample ratio and use a double-sided size selection approach to efficiently remove primer and adapter-dimer impurities smaller than 100 bp, improving NGS library purity.

Only 80% ethanol (freshly prepared) is needed. The formulation does not contain phenol, chloroform, or other organic solvents, ensuring safe and non-toxic operation.

The beads can be used for general PCR product purification, restriction enzyme digest recovery, plasmid purification, and other molecular biology experiments. It is a universal DNA purification bead kit.

It can only be kept at room temperature (25 degrees C) for 1-3 days. For long-term storage, it must be kept at 2-8 degrees C. Prolonged room temperature storage accelerates bead sedimentation and degradation.

Calibrate pipettes to ensure accurate dispensing, strictly follow the protocol’s bead ratios, and thoroughly vortex the bead suspension before dispensing.

The beads in this kit are single-use consumables. Once DNA is adsorbed, it cannot be desorbed or regenerated. Reuse is not recommended as it would affect subsequent selection performance.

1. Ensure that the YALEPIC^® Blood DNA/RNA Storage Tube (PQ)  is at 18−25˚C prior to use and properly labeled with specimen identification.
2. If theYALEPIC^® Blood DNA/RNA Storage Tube (PQ)  is the only tube to be drawn, blood should be drawn into a “Discard Tube” prior to drawing blood into the  YALEPIC^® Blood DNA/RNA Storage Tube (PQ)  so the interior volume of the blood collection set used during phlebotomy can be primed. Otherwise, the  YALEPIC^® Blood DNA/RNA Storage Tube (PQ)  should be the last tube drawn in the phlebotomy procedure.
3. Use a soft needle for standard blood collection, following national standards for medical equipment.
4. Allow at least 10 seconds for a complete blood draw to take place. Ensure that the blood has stopped flowinginto the tube before removing the tube from the holder. The YALEPIC^® Blood DNA/RNA Storage Tube (PQ)  is designed to draw 2.5 ml of blood into the tube.
5. After removing the needle, gently invert the tube 10 times to mix the blood with the preservative.(Note: Avoid insufficient or excessive mixing to maintain preservation efficacy.)
6. Store the YALEPIC^®Blood DNA/RNA Storage Tube（PQ）upright at room temperature (18−25˚C) for a minimum of 2 hours and a maximum of 72 hours before processing or transferring to refrigerator (2−8˚C) or freezer (−20˚C). The YALEPIC^®  Blood DNA/RNA Storage Tube（PQ） can be stored at −20˚C and below. If tubes are to be kept at temperatures below −20˚C, freeze them first at −20˚C for 24 hours, then transfer them to −70˚C or −80˚C. (Note: Thaw the YALEPIC^® Blood DNA/RNA Storage Tube (PQ) in a wire rack at ambient temperature (18–25˚C) for approximately two hours. Do not thaw the Storage Tubes at temperatures above 25˚C. )
7. Transport or store the sample according to the specified conditions and precautions.
8. For immediate RNA extraction after collection, allow 2 hoursafter mixing before proceeding with RNA extraction.

No. The core advantage of the product is liquid nitrogen-free processing. Fresh samples can be placed at room temperature and transported at room temperature after immersion, without the need for immediate cryogenic snap-freezing. Laboratories without liquid nitrogen equipment can use it normally.

Stable for 7 days at room temperature, 4 weeks at 4 degrees C, and long-term storage at -20 degrees C or -80 degrees C ultra-low temperature. Repeated freeze-thaw will not cause DNA/RNA degradation.

Yes. The proprietary formulation simultaneously and stably protects both DNA and RNA within tissue cells, eliminating the need for two separate preservation reagents, providing one-stop nucleic acid dual research capability.

Compatible with the vast majority of research samples including young plant tissue, animal tissue, cultured cells, suspended leukocytes, viruses, bacteria, insects, etc., particularly suitable for sensitive tissues rich in nucleic acid content.

Full room temperature transport is supported. For daily storage, simply keep sealed at 4-37 degrees C in the dark, with a shelf life of 2 years. No refrigeration or freezing is required for the reagent stock solution.

No. The reagent formulation contains no interfering components, effectively preserving the original nucleic acid structure. It is compatible with all types of nucleic acid extraction kits, PCR, gene expression analysis, sequencing, and other downstream experiments, with accurate and unbiased results.

Available in 100 ml/bottle and 500 ml/bottle specifications. The smaller size is suitable for individual small-batch experiments, while the larger size offers better cost-effectiveness for research groups and laboratories for routine stocking. For pre-dispensed sample storage tubes, choose YALEPIC Tissue DNA/RNA Storage Tube CAT#YS21072.

The formulation is safe and non-toxic, non-irritating, and non-corrosive. Standard laboratory protective measures are sufficient. It is user-friendly for laboratory personnel and will not contaminate sample nucleic acids.

Liquid nitrogen snap-freezing can instantly stop enzyme activity, but transport requires dry ice throughout, which is costly and not user-friendly for at-home sampling. This preservation fluid achieves a chemical snap-freeze effect at room temperature, stable for 14 days at room temperature, then can be directly couriered to a central laboratory and transferred to -80 degrees C. Most metatranscriptomic data show no significant difference in downstream RNA-seq gene expression profiles between the two methods, but the advantage in logistics convenience is significant.

Loose stool has high water content; it is recommended to appropriately increase the ratio of preservation fluid to stool, such as 1:4 or 1:5 (w/v), and vortex thoroughly. If the sample amount is small, it can also be collected directly into a collection tube pre-loaded with preservation fluid, where the liquid protectant quickly encapsulates and protects free RNA. Validation data show that watery stool can also be stably preserved at room temperature for 14 days.

We compared fresh frozen samples with preservation fluid samples stored at room temperature for 7 days using metatranscriptomic sequencing. PCoA and differential gene expression analysis both showed high similarity between the two groups (correlation >0.95), indicating that this preservation fluid effectively inhibits in vitro transcription of bacterial communities, avoiding ex vivo stress expression shifts.

Compatible. It is recommended to centrifuge the stool-preservation fluid suspension, discard the supernatant, and then lyse with TRIzol reagent. If TRIzol is added directly, the high content of preservation fluid components may slightly affect phase separation efficiency; an additional PBS wash can eliminate this effect. Using a silica column or magnetic bead-based stool RNA extraction kit is more recommended, as it yields higher purity and quantity of RNA.

Conventional electrophoresis shows clear 18S and 28S rRNA bands (eukaryotic samples) or 23S/16S bands (prokaryotic samples), with RIN values generally no less than 7. We have performed stool metatranscriptomic library construction and sequencing in multiple client projects. After rRNA removal, the proportion of effective reads is excellent, and gene coverage and expression correlation meet the requirements for high-impact publication.

The preservation fluid can rapidly inactivate most enveloped viruses and some non-enveloped viruses, significantly reducing biosafety risks. Samples comply with UN3373 Category B biological substance transport requirements. However, sealed leak-proof collection tubes must be used, outer packaging must be labeled according to biosafety regulations, and there are no special requirements for room temperature courier shipping.

Not recommended. Repeated opening and freeze-thaw will introduce moisture, oxidation, and microbial contamination, while physical disturbance may activate residual nucleases. It is recommended to aliquot into single-use tubes after the first mixing, store sealed at -80 degrees C, and use as needed.

Ready-to-use collection tubes in 5 ml and 30 ml sizes are available, pre-filled with sterilized and filtered preservation fluid, with an attached sampling spoon. Suitable for different volumes of stool samples, with custom labeling and packaging supported. Contact sales or authorized distributors for details.

Some products on the market are high-viscosity gels that do not easily infiltrate loose stool; others require -20 degrees C storage and transport. YALI Biotech’s product is a low-viscosity liquid that quickly coats stool of all consistencies, truly enabling room temperature transport, with competitive pricing. Comparative experiments show lower transcriptional induction effects on the metatranscriptome, with excellent RNA yield and integrity.

Regular EDTA blood collection tubes can only preserve blood samples for a short period (typically hours) and cannot effectively inhibit nucleated cell apoptosis. Over time, apoptotic cells release large amounts of genomic DNA into plasma, diluting the true cfDNA signal and affecting downstream detection sensitivity. YALEPIC cfDNA storage tubes contain specialized cell protectants that stabilize nucleated cells, prevent apoptosis and genomic DNA release, ensuring cfDNA concentration remains stable for up to 14 days.

YALEPIC cfDNA storage tubes are designed to extend the pre-analytical processing window. After blood collection, cfDNA can be stably preserved for up to 14 days at 4-37 degrees C, eliminating the need for immediate processing. This provides great convenience for cross-regional sample transport, multi-center clinical trials, and centralized sample processing.

Please collect blood to the exact volume marked on the tube – 5 ml for the 5 ml specification and 10 ml for the 10 ml specification. Over- or under-collection will disrupt the ratio of blood to protectant, potentially affecting cfDNA preservation. Insufficient volume may cause excessive protectant concentration affecting cells osmotically; excessive volume may result in insufficient protectant to fully inhibit nuclease activity and cell apoptosis.

Yes. After blood collection, immediately invert the tube gently 8-10 times to ensure thorough contact between blood and protectant. Proper mixing: rotate wrist 180 degrees completely, turning palm-inward to palm-outward in a smooth, steady motion. Avoid vigorous shaking to prevent hemolysis and blood cell damage. Delayed or insufficient mixing may result in uneven protectant distribution, potentially affecting preservation.

YALEPIC cfDNA storage tubes are primarily designed for stable preservation of cfDNA.

Whole blood from YALEPIC cfDNA storage tubes is processed to obtain plasma via standard plasma separation protocols. The separated plasma is compatible with most commercial cell-free DNA extraction kits, including but not limited to YALEPIC, Qiagen, Thermo, and other mainstream products. Extracted cfDNA can be directly used for downstream PCR, qPCR, digital PCR, NGS, and other high-sensitivity detection.

Glass offers excellent airtightness, chemical inertness, and biocompatibility, suitable for long-term storage and applications with strict storage requirements, but is heavier and breakable. PET plastic is lightweight with excellent shatter resistance, ideal for cold chain transport and air express, effectively reducing breakage during transport. Choose based on transport distance, storage duration, and logistics method.

Store unused tubes at 4-30 degrees C in a dry, dark environment. Avoid freezing and excessive heat. Use before the expiration date on the packaging – after expiration, vacuum and additive activity may decrease. Take appropriate insulation or protective measures when transporting unused tubes under extreme temperature conditions.

YALEPIC cfDNA storage tubes have CE-IVD certification or domestic NMPA registration.

The product has a stable working temperature of 4-37 degrees C. Room temperature indoor and room temperature logistics transport are both suitable, with no environmental temperature limitations throughout the year. No cold chain or refrigeration is needed.

Under standard 4-37 degrees C conditions, urine cell-free cfDNA can be stably preserved for 7 days, meeting the needs for cross-province delivery and batch sequencing experimental cycles.

Compatible with hospital clinical testing, non-invasive prenatal genetic screening, tumor early screening liquid biopsy, high-throughput gene sequencing, PCR research experiments, and biobank specimen storage.

Customization of tube volume and packaging quantity is supported. Bulk procurement can be matched with exclusive pricing. In-stock 10/50/100 ml specifications can be shipped the same day.

Formulated with EDTA anticoagulant plus composite nucleic acid protectant, it inhibits urinary nuclease degradation of cfDNA while fixing exfoliated urinary tract cells, preventing genomic DNA release from contaminating cell-free nucleic acid samples.

Unused empty tubes should be stored at 4-25 degrees C in a cool, dry place, with a manufacturer shelf life of 12 months. Each box’s outer packaging indicates the expiration date.

Fill the tube with urine to the corresponding specification. After sampling, invert and shake 5-8 times to thoroughly mix the urine with the protective fluid in the tube, ensuring nucleic acid stabilization.

Compatible with mainstream domestic and imported magnetic bead and spin column-based cfDNA extraction kits. YALI Biotech’s YALEPIC magnetic bead-based urine cell-free DNA extraction kit is prioritized, compatible with sample preparation for high-throughput sequencing across various platforms.

Minor precipitate in normal urine does not affect cell-free nucleic acid stability. As long as nucleic acid extraction is completed within the 7-day window, normal instrument testing can be performed.

Domestic in-stock delivery with shorter lead times and lower procurement costs, with equivalent 4-37 degrees C room temperature preservation performance. Supports on-demand customization, with more convenient after-sales technical support.

The tube interior is sterilized, complying with clinical sample collection standards. It can be used for clinical in vitro testing and research project sample collection.

Short-term environmental temperatures above 37 degrees C do not affect the 7-day basic preservation effect.

Specifically designed for bronchoalveolar lavage fluid (BALF) and pulmonary alveolar fluid samples. Not suitable for blood, sputum, tissue, or other sample types.

No. The storage tube supports 4-37 degrees C ambient transport without cold chain. Room temperature transport does not affect cfDNA integrity, eliminating the need for dry ice or refrigerated containers and reducing logistics costs.

At 4-37 degrees C, cfDNA in the sample remains stable for 14 days, and cellular genomic DNA can be preserved for up to 1 year, meeting both short-term transport and long-term sample retention needs.

No. Within the 4-37 degrees C range, temperature variations over 7 days show no significant decline in cfDNA concentration. The tube has strong resistance to environmental temperature fluctuations, suitable for long-distance cross-regional sample submission.

Yes. After collection, BALF samples require no special processing and can be directly extracted for cfDNA using standard commercial nucleic acid extraction kits. Fully compatible with NGS, PCR, mNGS, and other downstream molecular detection experiments.

No. The specialized protective formula only inhibits nuclease degradation and maintains cell integrity, with no nucleic acid-inhibiting components. It does not interfere with enzymatic reactions or gene sequencing results, ensuring high detection reproducibility.

Catalog number YS21032, single tube capacity 10 ml, standard packaging 100 tubes/box, supporting bulk procurement and routine laboratory stocking.

No. The storage tube strongly maintains nucleated cell integrity, preventing cell lysis and genomic DNA release, avoiding cfDNA dilution and ensuring accurate gene detection data.

No. The product is compatible with 4-37 degrees C room temperature transport and storage, without cold chain or refrigeration. Room temperature logistics is sufficient, and cfDNA stability is not affected by temperature fluctuations within 7 days.

Under 4-37 degrees C room temperature conditions, cfDNA can be stably preserved for 14 days, and genomic DNA in the sample can be stably preserved for up to 1 year, meeting sample retention and batch delivery needs.

No. The built-in protectant has no nucleic acid inhibition, does not interfere with enzymatic reactions, and is compatible with mainstream commercial nucleic acid extraction kits. The extracted product has complete purity, is compatible with PCR, NGS, and all downstream detection, with accurate and unbiased results.

Yes. The proprietary formulation firmly maintains the integrity of nucleated red blood cells and nucleated cells, inhibiting cell lysis and preventing gDNA release into cfDNA, reducing detection background interference at the source.

Catalog number YS21031, specification 10 ml/tube, 100 tubes/box, standard industrial packaging. Bulk purchasing by hospitals, laboratories, and testing institutes is supported, with proper certifications and COA quality reports available.

In a dark, dry environment at 10-30 degrees C, unopened products have a shelf life of up to 18 months from the manufacturing date. Storage conditions are flexible, with worry-free stocking.

This product has a CSF-specific formulation and is not recommended for blood, urine, bronchoalveolar lavage fluid, or other body fluids. Our company has dedicated cfDNA storage tubes for other body fluids available for inquiry and purchase.

The operation is simple. Just add the collected CSF directly to the storage tube. No additional reagents are needed, and no centrifugation pretreatment is required. Clinical healthcare workers and laboratory personnel can perform it quickly.

Absolutely not. This is the core advantage of our product. The YALEPIC Cervical Exfoliated Cell Storage Tube can stably protect DNA in samples at 4-37 degrees C ambient temperature for up to 2 years. Samples can be sent directly to the testing center at room temperature without cryogenic freezing or dry ice transport, which is very convenient.

The storage tube is broadly compatible with standard-sized cervical brushes and cervical swabs on the market. Simply break the swab head into the preservation fluid in the tube after sampling.

Yes. This preservation fluid preserves intact genomic DNA, which in addition to various HPV genotyping and quantitative PCR tests, is also suitable for DNA methylation detection, gene mutation analysis, chromatin immunoprecipitation, and other downstream molecular biology experiments requiring high DNA quality. However, note that the preservation fluid lyses cells, so it is not suitable for Pap smear examinations requiring intact cell morphology. To preserve intact cell morphology, choose YALEPIC Cervical Exfoliated Cell Storage Tube Type I-N CAT# YS21081.

Although the preservation fluid has undergone safety assessment, it still contains chemical components that inhibit microorganisms and nucleases. If splashed on skin, immediately rinse with copious amounts of running water. If splashed in eyes, rinse and seek medical attention promptly. If spilled on clothing, it may leave stains; wash as soon as possible using standard laundering.

One cervical exfoliated cell storage tube is designed for one cervical swab sample. Placing multiple swabs in the same tube compromises sample integrity and traceability, and may affect cell lysis and DNA release efficiency, resulting in test results that cannot be accurately matched to individuals. Please strictly follow the one-person-one-tube principle.

Our validated stability period is 2 years. Beyond this period, large DNA fragments may begin to slowly degrade. Although samples slightly past the expiration date may still detect some target sequences, we strongly recommend completing testing within the 2-year validity period to ensure high accuracy and reproducibility of results, especially for low-abundance viral load samples. For further information about YALEPIC storage tube test data in your specific experimental workflow, or to inquire about product pricing and trial sample applications, please contact our technical team directly.

Absolutely not. This is the core advantage of our product. The YALEPIC Cervical Exfoliated Cell Storage Tube can stably protect DNA in samples at 4-37 degrees C ambient temperature for up to 2 years. Samples can be sent directly to the testing center at room temperature without cryogenic freezing or dry ice transport, which is very convenient.

The storage tube is broadly compatible with standard-sized cervical brushes and cervical swabs on the market. Simply break the swab head into the preservation fluid in the tube after sampling.

Yes. This preservation fluid preserves intact genomic DNA, which in addition to various HPV genotyping and quantitative PCR tests, is also suitable for DNA methylation detection, gene mutation analysis, chromatin immunoprecipitation, and other downstream molecular biology experiments requiring high DNA quality. However, note that the preservation fluid lyses cells, so it is not suitable for Pap smear examinations requiring intact cell morphology. To preserve intact cell morphology, choose YALEPIC Cervical Exfoliated Cell Storage Tube Type I-N CAT# YS21081.

Although the preservation fluid has undergone safety assessment, it still contains chemical components that inhibit microorganisms and nucleases. If splashed on skin, immediately rinse with copious amounts of running water. If splashed in eyes, rinse and seek medical attention promptly. If spilled on clothing, it may leave stains; wash as soon as possible using standard laundering.

One cervical exfoliated cell storage tube is designed for one cervical swab sample. Placing multiple swabs in the same tube compromises sample integrity and traceability, and may affect cell lysis and DNA release efficiency, resulting in test results that cannot be accurately matched to individuals. Please strictly follow the one-person-one-tube principle.

Our validated stability period is 2 years. Beyond this period, large DNA fragments may begin to slowly degrade. Although samples slightly past the expiration date may still detect some target sequences, we strongly recommend completing testing within the 2-year validity period to ensure high accuracy and reproducibility of results, especially for low-abundance viral load samples. For further information about YALEPIC storage tube test data in your specific experimental workflow, or to inquire about product pricing and trial sample applications, please contact our technical team directly.

No significant changes will occur. The design goal of this product’s preservation fluid is to stabilize stool microbial DNA at room temperature (0.05), making it highly suitable for multi-center cohort studies requiring long-distance shipping or inability to process immediately.

Absolutely. YALEPIC preservation fluid protects DNA integrity, preventing target gene degradation during storage. Combined with an optimized stool DNA extraction kit, the extracted DNA can be directly used for absolute quantitative qPCR, ddPCR, or quantitative PCR detection, with accurate, reliable results and good reproducibility.

This preservation fluid is compatible with mainstream stool DNA extraction methods. We recommend using YALI Biotech’s YALEPIC stool genomic DNA extraction kit (or similar magnetic bead/spin column kits), which is specifically optimized to remove preservatives and PCR inhibitors from the preservation fluid, yielding the highest quantity and purity of DNA for NGS library construction.

Absolute precision is not required, but a sample close to 0.5 g (approximately the size of a soybean) is recommended. Insufficient sampling may result in insufficient DNA yield. Excessive sampling (>1 g) may result in relatively insufficient preservation fluid, with the sample unable to be completely immersed, and cells in some areas may be incompletely lysed or undergo putrefaction, affecting long-term preservation. The storage tube wall has a recommended fill line for reference.

Yes. The preservation fluid formulation contains effective components such as guanidine salts, which have been validated to efficiently inactivate enveloped viruses, including coronaviruses and influenza viruses. This minimizes the risk when processing potentially infectious stool samples from clinical or environmental sources after opening, protecting laboratory personnel safety.

Although designed specifically for stool, its formulation also provides similar DNA protection for samples with complex microbial communities such as animal intestinal contents, sewage sludge, or compost. However, it may not be effective for soil (due to humic acid interference), and food samples may require additional validation due to matrix interference. Self-testing or consulting technical support is recommended.

Suitable for all populations including adults, children, and infants. Using a non-invasive oral mucosa scraping method, no blood draw is needed. It is trauma-free, painless, gentle, and non-irritating, suitable for all ages.

Under standard 4-37 degrees C room temperature conditions, oral sample DNA can be stably preserved for up to 2 years. It can withstand short-term summer high-temperature exposure and long-distance logistics high-temperature environments. The specialized preservation fluid effectively inhibits nucleases, preventing DNA degradation and fragmentation.

No cold chain or refrigeration/freezing required. The entire process of transport, sampling, and long-term storage can be completed at 4-37 degrees C room temperature, significantly saving cold chain logistics and storage costs.

After sampling, break the swab head at the pre-scored breakpoint, completely immerse it in the preservation fluid in the storage tube, and tighten the cap. The operation is simple, requires no additional tools, and effectively prevents sample contamination.

Compatible with the vast majority of downstream molecular biology experiments, including conventional PCR, qPCR, gene chip analysis, next-generation sequencing (NGS), SNP genotyping, genetic disease screening, paternity testing, and more.

Model YS21052 specification is 2 ml/tube, with each tube containing 1 ml of preservation fluid, 50 tubes/box. Bulk procurement by laboratories and testing institutions is supported. Contact customer service for bulk pricing and customization services.

Avoid eating, drinking, chewing gum, or smoking for 30 minutes before sampling. Rinse the mouth with clean water. During sampling, avoid touching teeth or tongue, and only scrape the inner buccal mucosa to ensure adequate sample cell concentration.

Unopened original products should be stored at room temperature in a cool, dry place, with a shelf life of 3 years. Avoid damp, direct sunlight, and hot, stuffy environments. No refrigeration is needed for normal storage.

The tube features a screw-sealed leak-proof cap design, with pressure-resistant and drop-resistant tube walls. The sealing performance is excellent, and no leakage or seepage will occur during routine courier transport or bumpy handling. Sample safety is guaranteed.

Conventional dry swabs are susceptible to environmental humidity, bacterial growth, and rapid DNA degradation. This product contains a dedicated DNA preservation fluid that can sterilize, inhibit enzymes, and lock in nucleic acids, with longer preservation periods and higher sample purity. No drying process is needed; samples can be sealed immediately after sampling, suitable for long-distance transport to remote testing facilities.

Within 30 minutes before sampling, the subject should avoid eating, drinking, smoking, or chewing gum. Rinse the mouth with clean water to remove oral debris. After waiting 2-3 minutes, relax the cheeks to allow natural saliva secretion, gently spit into the collection funnel or directly into the storage tube until the saliva (excluding bubbles) reaches the marked scale line (approximately 2-3 ml). Tighten the cap and invert 10-15 times to ensure thorough contact between saliva and preservation fluid.

This product is specifically designed for stable room temperature preservation. After collection and mixing, samples can be stably stored at 4-37 degrees C for up to 2 years, without the need for immediate extraction. This makes it highly suitable for scenarios requiring centralized transport and batch processing after sample collection (such as multi-center clinical trials, large cohort studies). Even after months at room temperature, high-purity, intact high-molecular-weight DNA can still be extracted.

The operation is simple, with no special steps required. Transfer the mixed fluid from the tube to a centrifuge tube, centrifuge and discard the supernatant, then use any commercial saliva/blood genomic DNA extraction kit (magnetic bead or spin column method) following standard protocols. The protectant does not affect proteinase K activity, and no additional washing is needed. We recommend using YALI Biotech’s matching extraction kit for optimal results.

Absolutely. The total DNA extracted from this storage tube is typically 10-50 micrograms (depending on individual variation), with A260/280 ratio of 1.8-2.0 and A260/230 ratio >1.8. Fragment size is predominantly >=20 kb, with high integrity. We have verified its full compatibility with Illumina, MGI, and other platforms for whole-genome sequencing (WGS), target capture sequencing, and whole-exome sequencing (WES), with uniform sequencing data coverage and low GC bias.

The YALEPIC saliva DNA storage tube provides highly competitive cost-effectiveness while maintaining equivalent DNA stability performance (2 years at room temperature), suitable for large-scale procurement. Additionally, our protectant formulation is compatible with the vast majority of commercial extraction kits, with no restrictions on downstream workflows, and is specially optimized for high-temperature long-distance transport, making it more suitable for logistics conditions in China and countries along the Belt and Road with high summer temperatures. Furthermore, YALI Biotech provides full-process technical support from collection, preservation, to extraction.

Yes. If long-term sample storage (over 2 years) or special cell culture is needed, it is recommended to transfer the mixed fluid to cryovials for storage at -20 degrees C or -80 degrees C, either after 2 years of stable room temperature storage or for long-term biobanking purposes. However, we note that room temperature storage already meets the vast majority of long-term research needs, and repeated freeze-thaw may actually affect DNA integrity.

This product has passed ISO 13485 system certification and has completed practical application validation at multiple third-party genetic testing companies. YALI Biotech provides the matching YALEPIC magnetic bead-based saliva genomic DNA extraction kit, enabling high-throughput operation on automated extraction instruments. Welcome to contact us for trial sample applications.

1)LFDR（μl）：DNA(μg) 剂量可以在1:1和5:1μl 之间进行调整。

2)LFDR（μl）：siRNA（pmol）剂量可以在0.02:1和0.15:1之间进行调整。

细胞密度过低导致细胞生长缓慢，对外来刺激变得较为敏感，使得转染毒性增高。细胞密度过高，会导致细胞发生接触抑制，加快细胞凋亡。细胞融合度达到60% – 70%时，转染可以取得较高的转染效率。可预实验优化细胞转染密度。

当转染体系中存在多聚阴离子聚合物时(例如：硫酸葡聚糖、肝素等)，转染将无法正常进行，因此应避免上述物质存在于细胞转染体系中。

应尽量使用适度传代接种的细胞系，并尽量在平行实验时保持细胞传代次数的一致性，同时需避免细胞培养时间过长。

导致转染时细胞毒性大的因素有很多，例如DNA的用量过大、转染试剂的用量过大、转染时细胞状态较差以及培养基中抗生素的加入等。建议严格按照所选择转染试剂的说明书进行操作，以避免细胞毒性大的问题。

一个是转染试剂的稳定性，另一个是基因的稳定性。转染试剂应按照说明书建议的保存温度及条件进行保存。核酸如短时间内连续使用，可放置于4℃保存。若超过10天不使用，建议置于-20℃或-80℃长期储存以降低核酸的降解速度。

当细胞同时转染DNA和siRNA时，建议使用分别转染的方式进行。即使用基因转染试剂分别与DNA和siRNA进行复合，再将两个复合物体系分两次分别加入到同一个装有细胞的实验孔中。

Yes. LFDR3 is a true DNA/RNA universal transfection reagent. The same formulation can efficiently transfect plasmid DNA, siRNA, mRNA, and miRNA mimetics. There is no need to purchase separate specialized reagents for different types of nucleic acids, making it particularly suitable for laboratories that need to conduct both gene overexpression and gene silencing research, significantly reducing reagent procurement costs and management complexity.

Yes. LFDR3 was designed from the outset with co-transfection scenarios in mind. By optimizing the formation conditions of transfection complexes, users can mix plasmid DNA and siRNA at a defined ratio and co-incubate with LFDR3 reagent to form co-transfection complexes containing both types of nucleic acids, enabling simultaneous overexpression and gene silencing in the same cells. For initial use, a 1:1 DNA:siRNA mass ratio is recommended for optimization.

LFDR3’s cytotoxicity is significantly lower than traditional liposome-based transfection reagents. Traditional cationic liposome transfection reagents at high concentrations easily cause cell membrane damage and endoplasmic reticulum stress, leading to cell death. LFDR3 uses an innovative low-toxicity polymer material formulation. Cell viability can be maintained above 90% at 24-48 hours post-transfection (varying by cell type). This is particularly important for the reliability of experimental results with sensitive cell lines (such as primary neurons, immune cells, and stem cells).

In addition to standard adherent cells, LFDR3 performs well in various hard-to-transfect cells, including suspension cells (Jurkat, K562), primary cells (primary neurons, primary fibroblasts), stem cells (mesenchymal stem cells MSC, embryonic stem cells ESC), and some tumor cell lines sensitive to chemical reagents. Transfection efficiency varies between cell lines; a pilot experiment to optimize transfection conditions is recommended before first use.

Yes. LFDR3 can effectively transfect suspension cell lines such as Jurkat (human T-cell leukemia cells) and K562 (human chronic myeloid leukemia cells). For suspension cell transfection: select cells in logarithmic growth phase, adjust cell density to approximately 5×10^5-1×10^6 cells/mL, add the transfection complex directly to the cell suspension after formation, and after 4-6 hours of incubation, optionally replace with fresh culture medium based on experimental requirements.

No. One of LFDR3’s key operational advantages is its excellent serum compatibility. After transfection complex formation, it can be added directly to complete culture medium containing 10% fetal bovine serum (FBS) for transfection. There is no need to wash cells with serum-free medium before transfection, nor to change the medium during transfection. This feature greatly simplifies the workflow, reduces operational errors and contamination risks, and is particularly suitable for high-throughput transfection screening experiments.

For most cell lines, the recommended ratio of DNA amount to LFDR3 reagent volume is 1:2 to 1:3 (i.e., 1 microgram DNA corresponds to 2-3 microL LFDR3 reagent). For initial use, gradient ratios of 1:2, 1:2.5, and 1:3 are recommended for pilot optimization. For sensitive cells or hard-to-transfect cells, it may be necessary to reduce the reagent amount to minimize cytotoxicity. For specific optimization protocols, please refer to the product manual or contact YALI Biotech technical support.

The LFDR3 transfection workflow is very streamlined, taking approximately 30 minutes to complete complex preparation and addition: (1) Dilute DNA/RNA (about 5 minutes); (2) Add LFDR3 reagent (about 5 minutes); (3) Incubate complex at room temperature (10-15 minutes); (4) Add complex directly to cell culture plate (about 5 minutes). No medium change is needed after transfection. Downstream operations can be performed 4-6 hours post-transfection based on experimental requirements. Compared to transfection reagents that require medium changes, a single experiment can save 30-60 minutes.

LFDR3 should be stored at 2-8 degrees C protected from light. Freezing is strictly prohibited (freezing causes reagent structural damage, severely affecting transfection performance). The shelf life is 24 months when unopened. After opening, continue storing at 2-8 degrees C, tighten the cap to prevent evaporation and contamination, and use within the shelf life. For larger experimental volumes, consider aliquoting to reduce repeated cap opening. Mix thoroughly before each use, avoiding vigorous shaking.

Suboptimal transfection results are typically related to the following factors: (1) Cell condition – cell density should be at 70-90% confluence in logarithmic growth phase; (2) Nucleic acid quality – ensure DNA/RNA purity is high, endotoxin-free, with accurate concentration; (3) Ratio optimization – the DNA:reagent ratio may need adjustment based on cell type; (4) Storage conditions – confirm the reagent has not undergone repeated freeze-thaw or prolonged exposure to room temperature. It is recommended to investigate systematically from the directions mentioned above. For more specific questions, contact YALI Biotech technical support for personalized experimental advice.

1)LFDR（μl）：DNA(μg) 剂量可以在1:1和5:1μl 之间进行调整。

2)LFDR（μl）：siRNA（pmol）剂量可以在0.02:1和0.15:1之间进行调整。

细胞密度过低导致细胞生长缓慢，对外来刺激变得较为敏感，使得转染毒性增高。细胞密度过高，会导致细胞发生接触抑制，加快细胞凋亡。细胞融合度达到60% – 70%时，转染可以取得较高的转染效率。可预实验优化细胞转染密度。

当转染体系中存在多聚阴离子聚合物时(例如：硫酸葡聚糖、肝素等)，转染将无法正常进行，因此应避免上述物质存在于细胞转染体系中。

应尽量使用适度传代接种的细胞系，并尽量在平行实验时保持细胞传代次数的一致性，同时需避免细胞培养时间过长。

导致转染时细胞毒性大的因素有很多，例如DNA的用量过大、转染试剂的用量过大、转染时细胞状态较差以及培养基中抗生素的加入等。建议严格按照所选择转染试剂的说明书进行操作，以避免细胞毒性大的问题。

一个是转染试剂的稳定性，另一个是基因的稳定性。转染试剂应按照说明书建议的保存温度及条件进行保存。核酸如短时间内连续使用，可放置于4℃保存。若超过10天不使用，建议置于-20℃或-80℃长期储存以降低核酸的降解速度。

当细胞同时转染DNA和siRNA时，建议使用分别转染的方式进行。即使用基因转染试剂分别与DNA和siRNA进行复合，再将两个复合物体系分两次分别加入到同一个装有细胞的实验孔中。

LFMR（μl）：mRNA(μg) 剂量可以在2:1和5:1μl 之间进行调整。

细胞密度过低导致细胞生长缓慢，对外来刺激变得较为敏感，使得转染毒性增高。细胞密度过高，会导致细胞发生接触抑制，加快细胞凋亡。细胞融合度达到60% – 70%时，转染可以取得较高的转染效率。可预实验优化细胞转染密度。

当转染体系中存在多聚阴离子聚合物时(例如：硫酸葡聚糖、肝素等)，转染将无法正常进行，因此应避免上述物质存在于细胞转染体系中。

应尽量使用适度传代接种的细胞系，并尽量在平行实验时保持细胞传代次数的一致性，同时需避免细胞培养时间过长。

可能是如下两个原因造成的：1）细胞状态不好，参看第4条进行优化。2）转染效率太高，毒性由高转染效率带来。可通过调整试剂和基因的比例来降低细胞毒性，适量减少转染试剂的用量，可以降低复合物颗粒表面过量的正电荷从而降低细胞毒性。转染效率高导致的细胞毒性大并不影响蛋白的生产，可以继续对蛋白产量进行监控。

转染效果稳定性取决于转染试剂的稳定性和基因的稳定性。转染试剂应按照说明书建议的保存温度及条件进行保存。基因如短时间内连续使用，可放置于4℃保存。若超过10天不使用，建议置于-20℃或-80℃长期储存以降低核酸的降解速度。

The automatic nucleic acid extraction instrument has three core advantages over traditional manual extraction: first, standardization – the automated process eliminates human operational errors, resulting in better experimental reproducibility; second, high efficiency – YALEPIC Pure 32 can complete the full extraction process for 1-32 samples in 30-40 minutes; third, safety – the fully enclosed operation and disposable consumables design effectively reduces the risk of operator exposure to hazardous samples, especially suitable for infectious disease sample processing.

The instrument supports a wide range of sample types, including saliva, nasopharyngeal swabs, oral swabs, dried blood spots, whole blood, serum/plasma, animal tissue, plant tissue, cell cultures, bacterial cultures, and more. When used with corresponding magnetic bead-based extraction kits, both DNA and RNA can be extracted simultaneously.

Yes. YALEPIC Pure 32 features an open reagent system design, compatible with mainstream magnetic bead-based nucleic acid extraction kits on the market (such as YALEPIC, Tianlong, Bioer, Shanghai Shenzhen, and other brands). Users can freely select the most cost-effective reagent combinations without being tied to specific consumable brands, significantly reducing long-term operating costs.

The maximum processing capacity is 32 samples per batch. Extraction time varies depending on sample type and reagent system, typically completing the full process from lysis to elution within 30-50 minutes. Users can flexibly set any quantity from 1 to 32 based on actual sample volume, and the instrument will intelligently adjust the processing workflow without wasting reagents and consumables.

YALEPIC Pure 32 extracts nucleic acids with excellent quality: OD260/280 ratio stably between 1.7-2.0, magnetic bead recovery rate >=98%, and purification reproducibility CV <3%. This performance fully meets the nucleic acid quality requirements of downstream PCR, qPCR, next-generation sequencing (NGS), microarrays, and other high-precision detection applications.

YALEPIC Pure 32 is equipped with multiple anti-contamination designs: (1) Disposable magnetic rod sleeve technology – a new magnetic rod sleeve is used for each run; (2) HEPA high-efficiency filtration system – continuously purifies the chamber air; (3) UV disinfection module – automatically disinfects after the run is completed; (4) Enclosed operation chamber – effectively isolates aerosol diffusion. These four layers of protection ensure complete isolation between samples and a safe operating environment.

The instrument operation is extremely simple. It features a 7-inch color touchscreen with a graphical interface and multiple preset extraction programs. Users simply select the corresponding program and press Start. It also supports custom programming and program storage, enabling set once, recall repeatedly functionality. Regular laboratory personnel can operate it independently after 30 minutes of familiarization, without the need for specialized instrument training.

The choice of extraction instrument model depends primarily on your daily sample processing volume. YALEPIC Pure 32 is a 32-channel instrument that can process 1-32 samples per run, making it ideal for clinical laboratories, CDC labs, and small-to-medium research labs processing 20-100 samples per day. If your sample volume is larger (e.g., over 200 samples per day), consider a 96-channel high-throughput model.

Routine maintenance is very simple: (1) Clean the chamber and magnetic rod surface after each use; (2) Run the UV disinfection program once per week; (3) Inspect magnetic rods for visible wear monthly; (4) Perform instrument calibration every six months. The instrument’s Mean Time Between Failures (MTBF) exceeds 5000 hours, with very low maintenance frequency and no need for professional after-sales personnel intervention.

YALI Biotech provides a standard 12-month warranty for the entire instrument (excluding consumables). After-sales service includes: nationwide response within 48 hours, remote technical support (8-hour response on business days), annual calibration service, and lifelong repair support. Users can call the official service hotline at 0512-66256010 or email info@yalibiotech.com for technical support.

Compared with other 32-channel nucleic acid extraction instruments on the market (such as Tianlong NP968, Bioer NPA-32P, etc.), the core advantages of YALEPIC Pure 32 are: (1) Open reagent system – consumable costs can be reduced by over 30% compared to closed systems; (2) High magnetic bead recovery rate (>=98%) and excellent reproducibility (CV <3%), directly ensuring downstream experimental quality; (3) Four-layer anti-contamination design, especially suitable for high-sensitivity detection scenarios; (4) Friendly pricing strategy, offering near-imported instrument performance at a more competitive price point.

Standard compatibility with 1-5 ml of plasma, serum, amniotic fluid, urine, and other cell-free body fluids. This is a dedicated kit for large-volume cfDNA extraction, meeting high-sample-volume enrichment needs.

Fully compatible. The product is specifically optimized for short-fragment cfDNA, with high short-fragment proportion, no amplification bias, high nucleic acid purity, and no genomic DNA contamination. It can be directly used for NGS library construction, high-throughput sequencing, and liquid biopsy experiments.

Supports both manual extraction and fully automated high-throughput workstation dual modes; A/B/C three pre-loaded reagent versions are also provided, requiring no manual system preparation, directly compatible with mainstream automated extraction equipment for more efficient batch processing.

Yes. The kit’s buffer system is compatible with plasma, serum, amniotic fluid, urine, and various cell-free body fluids. It effectively removes urine impurities and amniotic fluid protein interference, stably purifying high-quality cell-free DNA.

No cold chain needed. Room temperature transport is supported, and daily storage at 10-30 degrees C room temperature in the dark is sufficient. No refrigeration or freezing required, making storage and use more convenient.

(1) Use cfDNA-dedicated blood collection tubes, and separate plasma as soon as possible after blood collection to avoid leukocyte lysis releasing gDNA contamination; (2) Appropriately increase the starting sample volume to 3-5 ml; (3) Strictly follow the protocol for lysis and binding steps, thoroughly mix and incubate magnetic beads, and slightly increase the elution volume to increase recovery.

Eluted cfDNA is recommended for short-term storage at 4 degrees C; for long-term storage, aliquot and store at -20 degrees C, avoiding repeated freeze-thaw to maximally maintain nucleic acid integrity.

The standard version consists of bulk reagents requiring self-preparation of the system; A/B/C pre-loaded versions are ready-to-use pre-formulated reagents, pre-dispensed in plates, for direct instrument use, saving experiment preparation time and more suitable for high-throughput automated experiments.

No. The kit uses a mild lysis magnetic bead purification method, entirely free of phenol, chloroform, beta-mercaptoethanol, and other toxic organic solvents. The experiment is safe and non-polluting.

Conventional stool kits are mostly optimized only for stool, while the YALEPIC SD kit uses a stool and soil dual-matrix universal formulation. The core breakthrough is the highly efficient removal of humic acid and PCR inhibitors. This enables difficult samples such as soil, compost, and sludge to yield clean, amplifiable DNA, truly achieving one kit covering both gut microbiome and environmental metagenomics research.

Yes. Alternatively, the NGS-dedicated NT-type product YALEPIC NT Stool & Soil Genomic DNA Isolation Kit (with RNase A) can be selected. Optimized lysis recovers gDNA from hard-to-lyse species in microbial communities including Gram-positive bacteria, fungi, and archaea. The de-inhibition step ensures no humic acid residue in the extracted product, enabling direct library construction for sequencing with stable coverage and alpha diversity metrics, avoiding sequencing failure or data bias caused by incomplete purification.

Humic acid strongly inhibits Taq polymerase activity, and even trace residue can cause Ct value delay or false negatives. If the eluted DNA appears pale yellow or transparent and colorless (rather than brownish-yellow), it indicates that humic acid has been removed, and conventional PCR and qPCR can be performed without additional column purification.

Fresh samples should be immediately stored at -80 degrees C, with no more than 2 freeze-thaw cycles. For samples containing preservation fluid (such as 95% ethanol, RNAlater, stool-specific preservation fluid), this kit is compatible; simply adjust the lysis buffer ratio according to the instructions.

For low-biomass samples, increasing the sample amount (up to 1 g) is recommended. Add 0.1 mm glass beads before lysis to assist mechanical disruption, and extend lysis at 56 degrees C for an additional 10 minutes. Use elution buffer pre-warmed to 65 degrees C and reduce the elution volume to 50 microL to significantly increase the final concentration.

(1) Dual-matrix universal, one kit handles both stool and soil, simplifying procurement; (2) Outstanding inhibitor removal capability, particularly suitable for downstream qPCR detection and library sequencing; (3) Localized full-process support, from trial sample application, technical protocol optimization to data analysis, YALI Biotech application scientists provide one-on-one assistance; (4) Highly competitive pricing, in-stock ready to ship, no long lead times, suitable for urgent project initiation.

Yes, the extracted genomic DNA covers the complete genomes of soil bacteria (including Bacillus and Actinomyces), archaea, and fungi. The lysis process uses dual physical and chemical methods, equipped with high-density grinding beads, effectively breaking down mold hyphae and chlamydospores to ensure fungal DNA yield. Commonly used for bacterial 16S rDNA, fungal ITS, and 18S rDNA amplification and sequencing.

It is recommended to immediately store at -80 degrees C after collection or use commercial stool preservation fluid to stabilize nucleic acids. For direct extraction of fresh samples, 200 mg is sufficient; frozen samples should avoid repeated freeze-thaw, and after weighing, add directly to the lysis tube. Liquid or semi-liquid stool (such as diarrheal stool) can be collected with a sterile cotton swab and directly immersed in lysis buffer to start extraction, without freeze-drying, making the operation user-friendly.

Smearing is commonly caused by humic acid residue or excessive mechanical shearing. This kit optimizes vortex time (horizontal vortex adapter at maximum speed for 5 minutes). If smearing occurs, reduce the vortex speed to medium intensity to avoid excessive fragmentation. Low yield is mostly seen with insufficient soil sample or high sandy soil; increasing the sample amount and extending lysis time is recommended.

Slight coloration often originates from residual humic acid or stereobilin, and small amounts of pigment have no effect on conventional PCR and qPCR. If the color is dark and the ratio is low, it is recommended to increase the elution buffer temperature to 65 degrees C for elution, or add an equal-volume chloroform extraction step before the binding step as an alternative (however, the NT-type already integrates highly efficient precipitation, and most samples do not require this step).

The core design of this kit is inhibitor removal. A proprietary precipitation solution forms insoluble complexes with humic acid, bile salts, and polysaccharides for removal by centrifugation. If Ct delay still occurs, it may be due to incomplete lysis resulting in low DNA yield or trace inhibitor residue. It is recommended to first increase the template to 2 microL of eluted DNA for re-testing; if the Ct advances, it indicates insufficient template. If delay persists, dilute the DNA 1:2 with elution buffer and re-amplify; dilution can eliminate trace inhibitor interference. Under normal operation, the Ct values of stool and soil DNA extracted with this kit in qPCR deviate from theoretical values by no more than +/-0.5.

This kit is suitable for total RNA extraction from Gram-negative bacteria (e.g., E. coli, Salmonella, Pseudomonas aeruginosa) and Gram-positive bacteria (e.g., Staphylococcus aureus, Bacillus subtilis, Streptococcus). For Gram-positive bacteria with thicker cell walls, the lysis time can be appropriately extended or combined with lysozyme pre-treatment for optimal lysis efficiency and RNA yield.

This kit uses an optimized wash system that effectively removes the vast majority of genomic DNA during extraction. The kit also includes RNase-free DNase I, enabling on-column gDNA digestion. RNA products after digestion typically show no gDNA amplification bands by PCR testing, meeting the stringent gDNA carryover requirements of downstream experiments such as RT-qPCR and RNA-Seq.

No. The YALEPIC Bacterial RNA Extraction Kit uses a guanidine isothiocyanate lysis and silica-membrane spin column purification approach throughout, without requiring phenol, chloroform, or other toxic organic solvents for extraction. The operation is safer and more environmentally friendly, substantially reducing the risk of personnel exposure to toxic chemicals, and is particularly suitable for routine laboratory and high-throughput extraction settings.

Under skilled operation, total RNA extraction from bacterial samples using this kit takes approximately 30 minutes for the complete workflow (including lysis, binding, washing, and elution steps). If on-column DNase I digestion is performed, add approximately 15 minutes. When processing multiple samples in batch, parallel operations can further improve experimental efficiency.

Using E. coli as an example, 10-30 microgram of high-purity total RNA can typically be obtained from 1×10^8 cells. For RNA purity, the A260/A280 ratio is generally between 1.9 and 2.1, with A260/A230 ratio >=2.0, indicating extremely low protein and organic solvent carryover. Agarose gel electrophoresis clearly shows 23S and 16S rRNA bands, with a 23S/16S ratio >=1.8, demonstrating excellent RNA integrity.

The recommended bacterial input is 1×10^6 to 1×10^8 cells; the maximum is not recommended to exceed 1×10^9 cells. Too little sample may result in low RNA yield; too much sample leads to incomplete lysis and column overload, reducing RNA purity and recovery. It is recommended to perform a pilot experiment based on bacterial species and growth state to determine the optimal sample input.

The total bacterial RNA extracted with this kit is of excellent quality and has been validated for a wide range of downstream applications including: RT-PCR, qRT-PCR (gene expression analysis), Northern Blot/Dot Blot (RNA detection), transcriptome sequencing (RNA-Seq), gene chip analysis (Microarray), Poly(A) mRNA selection, in vitro translation, RNase protection assays, and molecular cloning (cDNA library construction).

DNase I should be stored sealed at -20 degrees C and avoid repeated freeze-thaw. It is recommended to aliquot DNase I into small volumes (e.g., one tube per use) upon receipt. Use one tube at a time and continue storing unused aliquots at -20 degrees C. Repeated freeze-thaw causes DNase I activity decline and affects gDNA digestion efficiency.

Common causes of RNA degradation and resolution suggestions: RNase contamination – ensure use of RNase-free tips, tubes, and work surfaces. Wear gloves and a mask throughout the operation. Delayed sample processing – bacteria should be lysed immediately after collection, or temporarily stored in RNA protection solution at -80 degrees C. Incomplete lysis – ensure lysis buffer is thoroughly mixed with bacterial pellet. For Gram-positive bacteria, extend lysis time or add a lysozyme treatment step. Elution buffer issues – be sure to use the kit-provided RNase-free Water for elution; avoid using non-RNase-free water.

Compared with similar products on the market, the core advantages of the YALEPIC Bacterial RNA Extraction Kit include: Safe and non-toxic – no phenol/chloroform extraction required throughout, safer operation. Included DNase I – contains DNase I and dedicated buffer, no additional purchase needed, all in one kit. High RNA quality – unique silica-membrane material ensures high RNA purity and integrity. Broad bacterial species compatibility – simultaneously suitable for both Gram-negative and Gram-positive bacteria. High cost-effectiveness – 50T specification at only 750 CNY, per-reaction cost of only 15 CNY; additional discounts for bulk purchase. Manufacturer direct supply – independently developed and produced by YALI BIOTECH, stable quality with timely technical support response.

This kit is designed for manual spin column operation. For high-throughput automated extraction, YALI BIOTECH offers a matching magnetic bead-based bacterial RNA extraction kit that is fully compatible with YALI BIOTECH’s automated nucleic acid extractors and other mainstream automated platforms, achieving high-throughput extraction of 96 samples per batch. Please consult our technical team for automated workflow recommendations.

YALI BIOTECH provides full professional technical support for all products. If you encounter any issues during use, you can obtain help through the following channels: Technical service hotline: 0512-66256010, Technical support email: info@yalibiotech.com, Official website online message: www.yalibiotech.com.

It can universally extract DNA from various biological samples including whole blood, dried blood spots, oral swabs, saliva, and fresh animal/plant tissues, without the need for additional reagent formulation adjustments, offering extremely strong versatility.

It supports compatibility with various conventional magnetic racks, automated nucleic acid extractors, and high-throughput nucleic acid extraction workstations. Both standard and pre-filled A/B/C models can be adapted for automated extraction workflows.

The extracted genomic DNA integrity can reach up to 150 kb, with good fragment integrity, no obvious degradation, and compliant purity. It is fully suitable for NGS next-generation sequencing, gene cloning, and other high-end molecular biology experiments.

No cold chain is needed. It supports ambient-temperature shipping and can be stored sealed at 10-30 degrees C room temperature, with convenient storage and transport that does not affect reagent activity or extraction performance.

Yes. The DNA purified with this kit is free of protein, salt, and phenol contamination, with stable purity and concentration, and can be directly used for qPCR, conventional PCR, and other quantitative and qualitative experiments.

The most likely causes are incomplete sample lysis, residual ethanol in the wash step, or incomplete magnetic bead mixing. Strictly following the manual’s procedural steps, extending lysis time, and thoroughly removing residual ethanol can significantly improve DNA yield and purity.

Using standardized production processes and nano-magnetic bead formulation, batch-to-batch variability is minimal with strong experimental reproducibility, suitable for long-term research projects and batch testing experiments.

Common causes of low plasmid yield and solutions include: (1) Low plasmid copy number – different vectors have significantly different copy numbers. For low-copy plasmids (e.g., pET series, pBR322, pACYC), increase culture volume and proportionally increase lysis buffer volumes. Preheat elution buffer to 65 degrees C to improve elution efficiency. (2) Insufficient bacterial biomass – ensure sufficient volume of overnight culture and ensure cells are in logarithmic growth phase (OD600 approximately 1.0-1.5). Culture time is recommended at 12-16 hours and should not exceed 16 hours. (3) Improper strain storage – long-term glycerol stock storage may lead to plasmid loss. It is recommended to streak or plate to activate the strain before extraction to stabilize yield. (4) Incomplete lysis – after adding resuspension buffer, vortex until the bacterial pellet is fully suspended with no visible clumps. After lysis, gently invert to mix, ensuring complete lysis while avoiding genomic DNA shearing. (5) Improper elution conditions – preheating elution buffer to 65-70 degrees C significantly improves elution efficiency. After adding elution buffer, let it stand for 2-5 minutes to allow full membrane interaction. A second elution can be performed to improve total recovery. (6) Concentration measurement error – when measuring concentration with NanoDrop or similar instruments, use elution buffer (not water) as the blank for zeroing.

Common causes of high endotoxin levels and solutions: (1) Excessive culture volume – too much biomass leads to incomplete lysis, insufficient endotoxin release, and reduced removal efficiency. Strictly follow the kit’s recommended culture volume. (2) Endotoxin removal solution not fully mixed – after adding endotoxin removal solution, thoroughly mix by inverting up and down (>=15 times recommended) to ensure efficient endotoxin binding and removal. (3) Improper operation – when aspirating the supernatant, avoid drawing up the lower layer containing endotoxin-binding complexes. Centrifugation temperature should not be below 23 degrees C, as low temperatures lead to incomplete phase separation.

Genomic DNA contamination is mainly caused by improper lysis step operation: (1) After adding lysis buffer, mix gently by inverting (do not vigorously shake or vortex) to avoid mechanical shearing that fragments genomic DNA into small pieces that co-purify with plasmid. (2) Lysis time should be strictly controlled within 5 minutes. When processing multiple samples, monitor the total time to avoid over-lysis. (3) The neutralization step should be mixed thoroughly but gently to ensure genomic DNA precipitates together with proteins and cell debris and is removed.

(1) Confirm that RNase A has been added to Buffer PA and thoroughly mixed. Buffer P1 with RNase A added should be stored at 2-8 degrees C; prolonged room temperature storage leads to enzyme activity decline. (2) If the culture volume is too large, total RNA in the bacterial pellet may exceed the digestion capacity of RNase A. Appropriately reduce the culture volume. (3) Ensure RNase A is within its shelf life and stored under proper conditions.

Buffers such as Buffer PB containing SDS are prone to crystallization or precipitation under low-temperature conditions. Heat in a 37 degrees C water bath until completely dissolved, mix well, and use normally; this does not affect extraction performance.

(1) Increase bacterial biomass and proportionally increase the volume of each lysis buffer component to ensure complete cell lysis. (2) Preheat elution buffer to 65-70 degrees C to improve elution efficiency of large plasmids from the silica membrane. (3) Appropriately extend incubation times for binding and elution steps (e.g., extend binding to 5 minutes and elution to 5-10 minutes) to increase extraction efficiency.

For short-term storage (1-2 weeks), store at 2-8 degrees C. For long-term storage, store at -20 degrees C or -70 degrees C, avoiding repeated freeze-thaw. Plasmid DNA should be dissolved in low-salt buffer (such as the kit’s Elution Buffer).

Yes. The plasmid extracted with this kit has extremely low endotoxin carryover (<0.1 EU/microgram), high purity, and stable quality, suitable for advanced transfection experiments in most cell lines (including endotoxin-sensitive cell lines and primary cells) without additional purification steps.

Not recommended. Using rich media such as TB for bacterial culture may elevate endotoxin levels beyond the removal capacity of the kit. Standard LB medium is recommended to ensure endotoxin carryover meets specifications.

EndA- (endonuclease I deficient) E. coli strains such as DH5alpha, TOP10, and XL10-Blue are recommended. These strains prevent plasmid DNA degradation by endonuclease I, yielding higher quality and quantity plasmid.

(1) After using Buffer PB, cap the bottle immediately to prevent CO2 in the air from reacting with NaOH, reducing lysis efficiency. (2) After adding Buffer PB, mix gently by inverting (do not vortex); lysis time should not exceed 5 minutes. (3) After adding ER (neutralization solution), mix gently by inverting immediately until a white flocculent precipitate appears, ensuring complete neutralization. (4) With the blue indicator present: when lysis is complete, the solution is uniformly blue; when neutralization is complete, the blue color disappears and a white precipitate forms – this color change is a key indicator for judging whether the operation has been performed correctly.

This kit offers the following differentiated advantages: (1) Lower endotoxin carryover: <0.1 EU/microgram, at the industry-leading level (most mainstream competitors are <0.1-1 EU/microgram), meeting higher transfection standards. (2) Faster extraction: complete workflow approximately 50 minutes, no ice bath required, more efficient than the 60-90 minutes of most competitors. (3) Visual blue indicator: helps users intuitively judge whether lysis and neutralization are complete, reducing experience-based judgment errors and ensuring batch-to-batch consistency. (4) High yield: 100-300 mL culture system can yield up to 2-3 mg plasmid DNA, superior to most competitors of the same specification. (5) No phenol/chloroform extraction: safer and more environmentally friendly operation, no ethanol precipitation required, simpler workflow.

This kit is specifically designed for dried blood spots, blood collection card specimens, and blood imprint samples, and is used exclusively for genomic DNA extraction from these sample types, meeting routine blood sample preparation needs in research laboratories.

It does not affect the core DNA extraction purity and integrity. The kit includes an optimized buffer system that efficiently removes most RNA impurities from the sample. If complete degradation of trace RNA is required, standard RNase A can be added separately to meet different experimental precision requirements.

No. Using an improved silica membrane spin column adsorption principle, the entire process avoids toxic organic solvents such as phenol and chloroform, and eliminates ethanol precipitation steps. The operation is safe and the workflow is more streamlined.

The standardized protocol completes the full genomic DNA extraction workflow including lysis, binding, washing, and elution from dried blood spot/card specimens in 40 minutes, efficiently saving experimental time.

The extracted high-purity genomic DNA can be widely used for restriction digestion, conventional PCR amplification, real-time qPCR, Southern blot hybridization, gene library construction, and other molecular biology experiments.

Room temperature transport is supported with no cold chain transport restrictions. For daily storage, simply place in a dry, dark environment at 10-30 degrees C room temperature. Storage conditions are flexible and easy to maintain.

No. The kit’s proprietary buffer system maximally removes contaminating proteins, lipids, and various PCR-inhibitory impurities. DNA purity meets standards with no significant experimental inhibition and good reproducibility.

Designed for 1-15 ml of overnight E. coli culture, compatible with routine experimental volumes without additional reagent ratio adjustments.

Standardized operation takes only 20 minutes. Maximum plasmid DNA yield per preparation can reach 100 micrograms, meeting routine experimental needs.

The kit includes a pre-mixed blue indicator that visually confirms cell lysis and neutralization completion through color changes, avoiding over-lysis or insufficient neutralization, reducing impurity contamination risk, and enabling visual operation.

Yes. The kit uses silica membrane purification technology to efficiently remove RNA, genomic DNA, and protein impurities. The extracted plasmid purity meets standards for direct use in restriction digestion, sequencing, PCR, in vitro transcription, and other downstream experiments without further purification.

No cold chain needed. Supports room temperature transport throughout. Store sealed at 10-30 degrees C upon receipt. Simple storage conditions without occupying refrigerator space.

Common causes include insufficient culture density, incomplete cell lysis, and inaccurate elution buffer application to the membrane center. Recommendations: use fresh culture with OD600 2.0-4.0, mix gently by inversion (avoid vigorous shaking), apply elution buffer to the silica membrane center and let stand 1 minute before centrifugation to significantly improve recovery.

The optimized formulation with specific silica membrane adsorption effectively removes RNA and genomic DNA impurities. Under normal operation, no significant residue is present, meeting standard molecular biology experiment requirements.

This kit is recommended for 30-50 mL of overnight bacterial culture (E. coli, such as DH5alpha, TOP10, etc.). For low-copy plasmids, it is recommended to appropriately increase the culture volume to boost yield. For high-copy plasmids, the culture volume can be appropriately reduced to ensure extraction efficiency.

Through a dedicated endotoxin removal filter and optimized buffer system, this kit controls endotoxin carryover to <=1 EU/microgram of plasmid DNA. This level meets transfection-grade standards, and the extraction product can be directly used for transient and stable transfection of mammalian cells (e.g., HEK293, CHO) and primary cells. If even lower endotoxin levels are required (e.g., <0.1 EU/microgram), options YC47015 (100-300 mL) and YC47018 (100-500 mL) plasmid extraction kits are available.

During the cell lysis and neutralization steps, the indicator undergoes a color change from deep blue to light purple to colorless in response to pH changes, helping operators intuitively judge whether lysis is sufficient and neutralization is complete. This is especially useful for less experienced experimenters, effectively reducing the risk of DNA loss or RNA/protein carryover due to incomplete neutralization or over-lysis.

The high-purity, endotoxin-free plasmid DNA extracted with this kit has been validated by multiple laboratories and is suitable for the following applications: transient and stable transfection of mammalian cells and sensitive cell lines; restriction enzyme digestion and ligation/transformation; Sanger sequencing and high-throughput NGS library construction; PCR, qPCR, RT-PCR, and multiplex PCR amplification; in vitro transcription and RNA synthesis; plasmid transformation and genetic modification of prokaryotic cells.

This kit is stored sealed at 10-30 degrees C room temperature, protected from light, with a shelf life of 18 months. It supports ambient-temperature shipping (recommended environmental temperature controlled at 4-35 degrees C) without cold chain, greatly reducing warehousing and logistics costs. Please calculate the shelf life based on the labeled production date; do not use beyond the expiration date to ensure extraction performance.

Plasmid yield is affected by multiple factors. Common causes include: Plasmid copy number – low-copy plasmids (e.g., pBR322-derived vectors) naturally yield less than high-copy plasmids (e.g., pUC series). Culture condition – insufficient culture time (OD600 6.0) both affect yield. Incomplete lysis – insufficient lysis time or inadequate lysis buffer volume prevents complete plasmid release. Excessive washing – missing ethanol addition in wash steps as instructed or excessive wash cycles may cause plasmid to be washed off the membrane. Elution temperature – preheat elution buffer to 65 degrees C before use; low-temperature elution reduces yield. Check the above aspects and follow the manual for standardized operation.

This kit is designed based on the alkaline lysis method and is specifically for plasmid extraction from Gram-negative bacteria (mainly E. coli). For Gram-positive bacteria (e.g., Bacillus subtilis, Staphylococcus aureus), due to differences in cell wall structure, lysis conditions require additional adjustments (e.g., lysozyme addition). This kit has not been specifically optimized for such scenarios and is not recommended for direct use.

RNase A has been pre-mixed into the lysis buffer of this kit, effectively degrading RNA in the bacterial culture during the lysis step. When the product is analyzed by agarose gel electrophoresis, no obvious RNA smear band is typically observed. If the lab is extremely sensitive to RNA carryover, additional RNase A (self-prepared) can be added after the resuspension step for enhanced treatment.

YALI BIOTECH provides a complete product line: mini-prep (1-5 mL culture), midi-prep (5-15 mL culture), (30-50 mL culture), and maxi-prep (100-300 mL culture), (100-500 mL culture) to meet different experimental scale needs. The main differences between this midi-prep kit and the mini-prep kit include: Processing scale – midi-prep supports larger sample volumes (30-50 mL vs. 1-5 mL). Yield – midi-prep yield can reach up to 300 microgram, while mini-prep is typically <45 microgram. Choose the appropriate specification based on experimental scale and downstream application needs.

This kit is routinely recommended for 1-5 mL of overnight culture. For low-copy plasmids, the culture volume can be appropriately increased to <=10 mL, and Buffer PA, PB, and PM2 reagent volumes should be strictly scaled up proportionally to ensure complete lysis, neutralization, and binding steps for improved yield.

Common causes include aged bacteria, overgrowth of culture, incomplete lysis, or insufficient binding/elution time. Recommendations: re-pick a fresh single colony for overnight culture (12-16 hours optimal), strictly control lysis time within 5 minutes, extend binding or elution time. Preheating Elution Buffer to 60 degrees C before use helps improve elution rate. For low-copy plasmids, appropriately increase culture volume and extend binding time.

This kit is pre-mixed with a blue/yellow indicator: the lysis buffer should normally be blue. If it turns yellow-green or yellow, this usually indicates abnormal buffer system pH or excessive sample addition. Reduce the culture volume and ensure all buffers are fresh and free of precipitate (if precipitate appears, dissolve in a 37 degrees C water bath before use). If the mixture does not become fully clear after neutralization, check whether neutralization is sufficient or mix again and centrifuge.

Yes. Gram-positive bacteria require cell wall disruption pre-treatment (e.g., lysozyme treatment). After cell wall disruption, follow this kit’s standard operating protocol (alkaline lysis method + spin column purification) for extraction.

For large plasmids >10 kb, 5-10 mL of culture is recommended, with PA/PB/PM2 Buffer volumes strictly increased proportionally. Binding and elution steps can be appropriately extended in reaction time. Use Elution Buffer preheated to 60 degrees C and avoid vigorous shaking to prevent large plasmid breakage.

This kit is shipped at ambient temperature (10-30 degrees C) and stored at room temperature. If Buffer PB shows precipitate before use, dissolve in a 37 degrees C water bath and mix thoroughly. The unopened kit has a shelf life of 12 months.

The maximum yield can reach 45 microgram of plasmid DNA (high-copy plasmid, 5 mL culture). Both high-copy and low-copy plasmids are applicable. For low-copy plasmids, increase culture volume and scale up reagent amounts to achieve optimal extraction efficiency.

Spin column clogging is typically caused by incomplete lysis or excessive culture volume. Recommendations: use a vortexer for thorough mixing during lysis to avoid residual bacterial clumps. Strictly follow the 1-5 mL recommended culture volume to avoid overloading. Ensure centrifugation force is >=12,000 x g.

The YALEPIC Ultrapure Plasmid DNA Mini Extraction Kit uses imported specialty silica membrane material and an optimized high-compatibility alkaline lysis buffer system, delivering up to 45 microgram yield with A260/A280 purity of 1.8-2.0. Combined with the blue/yellow indicator for visualized operation, the extraction efficiency and purity meet the requirements of downstream applications such as molecular cloning, sequencing, and transfection, with significantly better cost-effectiveness than comparable imported and joint-venture plasmid extraction products.

Eluted plasmid DNA can be stored at -20 degrees C for short-term (within 1 week). For long-term storage, aliquot and store at -80 degrees C to avoid repeated freeze-thaw. Use sterile, nuclease-free ddH2O or Elution Buffer for elution to improve storage stability.

This kit provides RNase A. Before use, add all RNase A to Buffer PA, mix well, aliquot, and store at 2-8 degrees C. Avoid RNase contamination and use RNase-free consumables during operation to further improve extraction quality.

When collecting saliva samples, please note the following: Avoid eating, drinking, smoking, or chewing gum for 30 minutes before collection to prevent affecting DNA quality; Rinse the mouth gently with clean water before collection to remove food residues; Collect 200-500 microL of fresh saliva as the starting sample volume (can be adjusted according to experimental needs); If extraction cannot be performed immediately after collection, mix saliva with saliva preservation fluid at the recommended ratio (typically 1:1), room temperature storage for weeks to months without affecting DNA quality; Avoid excessive bubbles in the saliva, as bubbles may cause inaccurate sample volume measurement.

The kit’s optimized buffer system can process 200-500 microL of saliva samples, with a recommended standard volume of 400 microL. For trace samples (below 200 microL), it is recommended to bring the volume up to standard with saliva preservation fluid or PBS before extraction, or proportionally reduce reagent volumes as instructed in the manual. For extremely trace samples (less than 50 microL), a DNA extraction kit specifically designed for trace samples is recommended. For further guidance, please contact YALI Biotech technical support.

Yes. The saliva genomic DNA extracted with this kit undergoes rigorous washing steps to thoroughly remove proteins, polysaccharides, lipids, and other PCR inhibitors. The A260/A280 ratio is typically 1.7-2.0, A260/A230 ratio >1.8, and DNA fragment size >15 kb, fully meeting the quality requirements for NGS library construction (such as Illumina, MGI platforms). Users have successfully completed whole-exome sequencing library construction and sequencing using saliva gDNA extracted with this product. For NGS applications, accurate DNA concentration quantification before library construction is recommended (Qubit fluorometry is recommended).

This kit is specifically designed for saliva samples but is also suitable for DNA extraction from oral swab samples. For swab samples, cut the swab head into a centrifuge tube, add lysis buffer and proteinase K, and vortex thoroughly to release cells. Subsequent steps are identical to the saliva sample protocol. For high-throughput extraction specifically designed for swab samples, please refer to other YALI Biotech nucleic acid extraction product lines.

This kit does not contain RNase A by default for the following reasons: (1) Some downstream applications (such as simultaneous DNA and RNA analysis, RNA interference studies, etc.) require preserving the RNA component during extraction; (2) A kit without RNase A provides greater experimental flexibility, allowing users to choose whether to add RNase A based on actual needs. If you need to remove RNA: Add an appropriate amount of RNase A solution (not provided with the kit, needs to be prepared or purchased separately) to the sample after the lysis step, incubate at 37 degrees C for 15-20 minutes, then continue with subsequent steps. If you need to preserve RNA while extracting DNA for downstream detection, a kit specifically designed for DNA/RNA co-extraction is recommended.

This kit can be stably stored for 12 months at 10-30 degrees C room temperature when unopened. After opening, all components can be used until the labeled expiration date under proper storage conditions (lysis buffer, wash buffer, and elution buffer at room temperature; proteinase K recommended at -20 degrees C). Tighten caps immediately after each use to prevent contamination and reagent evaporation. After opening Wash Buffer 2 concentrate, immediately add the specified volume of anhydrous ethanol, mark the date of ethanol addition, store at room temperature after adding ethanol, and use within 6 months. Refer to the product packaging label for the exact expiration date.

This kit includes an optimized wash system that effectively removes proteins, polysaccharides, and other enzymatic reaction inhibitors through two wash steps. The extracted DNA can be directly used for PCR, qPCR, and other enzymatic reactions without additional purification. To verify the presence of PCR inhibitors, the following methods can be used: (1) Internal reference amplification control method: Mix a known concentration of standard DNA template with an equal amount of extracted DNA for PCR amplification and compare amplification efficiency; (2) A260/A230 ratio method: A260/A230 ratio <1.8 suggests possible guanidine salt, polysaccharide, or other inhibitor residue; (3) Concentration gradient dilution method: Serially dilute the extracted DNA for PCR and observe whether the amplification curve shows a linear relationship.

This kit is designed for manual operation based on the spin column method, suitable for small-to-medium throughput sample processing (50-prep specification). For high-throughput automated extraction solutions, YALI Biotech offers the magnetic bead-based saliva genomic DNA extraction kit YALEPIC MagEVO Saliva DNA Extraction Kit CAT#YM26010. Please visit the YALI Biotech website or contact sales for detailed information.

The purified saliva genomic DNA can be stored long-term under the following conditions: -20 degrees C storage: Dissolved in elution buffer or nuclease-free water, can be stored for 6-12 months, suitable for medium-to-short-term storage; -80 degrees C storage: Can be stored long-term (several years), aliquoting into small tubes is recommended to avoid DNA degradation from repeated freeze-thaw; 4 degrees C short-term storage: DNA to be used within 3-5 days after extraction can be temporarily stored at 4 degrees C.

For low-abundance DNA saliva samples (such as from elderly individuals, Sjogren’s syndrome patients, long-term medication users, etc.), the following optimization measures can be tried to improve yield: (1) Increase starting sample volume: Appropriately increase the saliva sample volume to 600-800 microL, provided the spin column capacity (typically <=30 microgram DNA) is not exceeded; (2) Optimize lysis time: Extend the lysis step to 15-20 minutes, and if necessary, increase the lysis temperature to 65 degrees C; (3) Reduce elution volume: Use 50 microL (instead of 100 microL) of elution buffer to increase DNA concentration; (4) Double elution and combine: Elute twice with 30-40 microL of elution buffer and combine the DNA solution from the collection tube; (5) Pre-warm elution buffer: Pre-warm the elution buffer to 65-70 degrees C before adding to the adsorption column to improve elution efficiency; (6) Extend elution incubation time: After adding elution buffer, let stand at room temperature for 2-5 minutes before centrifugation to ensure complete DNA dissolution.

Yes. This kit is explicitly labeled as suitable for genomic DNA extraction from saliva/preservation fluid mixture samples. Commercial saliva preservation fluids typically contain components that stabilize cell membranes and inhibit nuclease activity, and are compatible with the kit’s lysis buffer system. It is recommended to consult the preservation fluid supplier before use to confirm compatibility with this kit. For untested preservation fluid brands, a pilot experiment with a small sample is recommended for verification.

Absolutely. The YALEPIC Swab Genomic DNA Isolation Kit is specifically optimized for PCR applications, with significant inhibitor removal performance. The extracted DNA can be directly used as a template for conventional PCR, qPCR, and multiplex PCR. Typically, 1-2 oral swabs yield sufficient genomic DNA for 100 PCR reactions. If the amplification Ct value is high, verify that the swab sampling was adequate, or reduce the elution volume to increase DNA concentration.

Yes. This kit is compatible with various swab samples containing guanidinium isothiocyanate or non-guanidine salt-based viral transport media and cell preservation fluids. For throat or nasal swabs with preservation fluid, simply take 200-400 microL of the fluid (centrifuge to collect the pellet) and follow the standard protocol to obtain high-purity DNA. Particularly suitable for re-testing human genomic DNA in positive samples during COVID-19 and influenza virus detection.

Low DNA yield is commonly caused by insufficient cell collection on the swab, short digestion time, or low elution efficiency. Recommendations: (1) Thoroughly scrape the buccal mucosa during oral swab sampling to ensure adequate cell count; (2) Extend proteinase K digestion at 56 degrees C to 1 hour; (3) Pre-warm the Elution Buffer to 60 degrees C before elution and incubate on the membrane for 3-5 minutes. If OD260/280 is low (<1.7), protein residue may be present; add an additional wash step or re-purify.

Yes. For nasopharyngeal swab samples that may contain both pathogen DNA (such as EBV, adenovirus) and host cells, this kit can unbiasedly co-extract total DNA. The extracted product can be directly used for viral DNA quantification and human housekeeping gene detection, meeting the need for same-tube extraction and multiplex detection, making it ideal for combined pathogen screening.

Yes. This kit has good recovery capability for trace DNA and is suitable for forensic biological specimens, fingerprint wipe swabs, and exfoliated cell extraction. For extremely low copy number samples, a 20-30 microL elution volume is recommended, along with Carrier RNA, to significantly improve trace DNA recovery efficiency. The product meets STR typing requirements.

A single extraction takes approximately 25-30 minutes (excluding digestion time), and the entire process can be completed at room temperature without low-temperature centrifugation. Standard operations include: proteinase K digestion (can be pre-warmed to accelerate), binding, two washes, and elution. All steps are performed at room temperature, with maximum centrifugation speed of ~13,000 rpm. A standard benchtop centrifuge is sufficient, making it ideal for field rapid detection vehicles or primary laboratories.

Yes. This kit (YALEPIC MagEVO Universal Plant DNA Extraction Kit) is specifically formulated for complex plant samples rich in polysaccharides and polyphenols. The magnetic bead method combined with a unique buffer system effectively removes polysaccharides, polyphenols, proteins, and other interferents. Extracted DNA has high purity (A260/A280 typically 1.7-2.0) and can be directly used for PCR, sequencing, and other downstream experiments. Typical suitable samples include cotton leaves, pine needles, ginkgo leaves, banana flesh, grape flesh, potato tubers, etc.

Yes. The kit offers pre-packaged specifications (Type A: 32T/48T/96T pre-filled plates) that seamlessly integrate with mainstream pipetting-based automated workstations and magnetic rod-based automated nucleic acid extractors. Up to 96 samples can be processed simultaneously, greatly improving throughput for large-scale breeding screening, germplasm resource identification, and other applications.

Yes. The extracted DNA has high purity, no protein or RNA contamination, and no PCR inhibitor residue. It can be directly used for PCR, Real-Time PCR, qPCR, Southern Blot, library construction, and high-throughput sequencing (NGS) and other downstream molecular biology experiments.

Low DNA purity is typically caused by: (1) Insufficient lysis, incomplete nucleic acid release; (2) Poor bead dispersion, adsorption capacity too strong or weak; (3) Insufficient washing, residual protein or salt; (4) Excessive sample input beyond kit capacity. Recommendations: optimize lysis conditions, thoroughly mix beads, strictly follow washing protocols, and use recommended sample amounts.

Possible causes and solutions: (1) Insufficient starting material – moderately increase sample amount within recommended range; (2) Insufficient lysis – ensure thorough liquid nitrogen grinding or tissue homogenization, mix immediately after adding lysis buffer; (3) Insufficient elution – pre-warm Elution Buffer to 65 degrees C or extend elution to 5 minutes; (4) Intrinsically low DNA content (e.g., old dried seeds) – optimize pre-treatment. For complex samples (rice, corn, etc.), keep starting material below 50 mg fresh weight or 10 mg dry weight.

For the magnetic bead method, bead aggregation or abnormal adsorption may result from high lysis buffer viscosity or uneven mixing. Recommendations: (1) Ensure thorough sample grinding and lysis to avoid high viscosity; (2) Vortex or invert to mix thoroughly before magnetic separation; (3) Appropriately reduce starting sample amount.

Recommended sample amounts: common plant leaves/fungi 20-100 mg; polysaccharide-rich tubers/roots 20-50 mg; seeds 10-30 mg; fruits 50-200 mg. For samples with extremely high polysaccharide/polyphenol content (banana flesh, grape flesh, cotton roots, etc.), start with low amounts and optimize gradually.

Main advantages of magnetic bead vs column-based methods: (1) Superior automation compatibility – magnetic beads seamlessly integrate with automated workstations for 96-well high-throughput operation, while columns are limited by centrifugation steps; (2) Larger DNA fragments (>30 kb) from magnetic beads, more suitable for long-fragment PCR and large-fragment library construction, vs typically <9 kb from columns; (3) More stable purification of polysaccharide/polyphenol samples, avoiding column clogging issues.

This kit uses superparamagnetic particle purification technology. Functionalized bead surfaces specifically adsorb nucleic acid molecules, with an external magnetic field enabling rapid nucleic acid enrichment and separation. Compared to traditional CTAB: (1) No chloroform or organic reagents needed, safe and environmentally friendly; (2) Significantly shorter operation time (1 hour vs 2-4 hours); (3) Supports automated high-throughput operation; (4) More stable and consistent purification of polysaccharide/polyphenol samples. CTAB’s advantage is lower cost, but it is clearly inferior in throughput, automation, and ease of operation.

In total DNA extracted from human fecal samples, the vast majority (over 80%) comes from gut microbes, with host DNA typically not exceeding 20%. The specific ratio varies depending on the sample source (human, mouse, rat, dog, cat, etc.) and individual differences.

It is recommended to process samples as soon as possible after collection. If same-day extraction is not possible, fecal samples should be frozen as soon as possible at -20 degrees C or -80 degrees C. For long-term preservation, dedicated fecal nucleic acid preservation tubes may also be considered.

Yes. The kit has been validated for fecal samples from a variety of animals, including humans, mice, rats, dogs, cats, horses, and cattle.

Fecal samples often contain PCR inhibitors such as bile salts, complex polysaccharides, and humic acids. This kit effectively removes these inhibitors through an optimized lysis system and wash protocol, ensuring that the extracted DNA can be directly used for downstream experiments such as PCR and qPCR without additional purification steps.

From approximately 0.2 g of human fecal sample, 5-25 microgram of total DNA can be extracted. From mouse or rat fecal samples (approximately 0.1 g), the extractable DNA amount typically falls within the aforementioned range.

Yes. The kit can simultaneously extract DNA from bacteria, fungi (including yeast and mold), actinomycetes, and various other microorganisms from soil samples.

Generally, 100-500 mg of soil sample is recommended, and the specific amount can be appropriately adjusted based on soil type. For soils with high humus content (e.g., humus-rich soil, wetland silt), the sample amount can be appropriately reduced. For sandy soil, the sample amount can be appropriately increased. It is recommended to conduct a small-scale pilot experiment based on soil type for initial use to determine the optimal sample amount.

Humic acids and fulvic acids in soil (especially humus-rich soil) are major inhibitors of PCR and qPCR. The unique buffer system and wash solution of this kit can efficiently remove these interfering substances. The extracted DNA is colorless and transparent, with no brown residue, and can be directly used for downstream molecular detection.

This is a normal phenomenon. If precipitate appears in the buffer (especially under low-temperature conditions), heat the buffer in a 37 degrees C water bath and mix thoroughly. Use after the precipitate has completely dissolved. Check that all reagents have returned to room temperature before use.

DNA yield from soil samples varies depending on soil type and microbial abundance. Generally, over 5 microgram of DNA can be obtained per gram of soil, with DNA fragment lengths primarily distributed between 20-30 kb.

This kit is suitable for fresh saliva samples, saliva samples with preservation fluid, and oral swab samples. The sample collection process is painless and non-invasive; subjects only need to spit an appropriate amount of saliva to complete sampling. It is suitable for batch testing in hospital laboratory departments and third-party medical testing institutes, as well as genetics research at research institutions and personnel screening at forensic identification agencies.

This kit can extract 2-10 micrograms of genomic DNA from 200-400 microL of saliva samples (varying with individual differences), with OD260/280 ratio stably between 1.8-2.0, fully demonstrating excellent product purity. The extracted genomic DNA can be directly used for qPCR quantitative analysis, NGS library construction, genotyping, STR analysis, gene chip detection, and other downstream experiments, without PCR inhibitor residue interference.

The manual operation workflow (including all steps of sample lysis digestion, magnetic bead binding, washing, and elution) takes approximately 60 minutes. Using an automated nucleic acid extraction instrument, extraction time for 96 samples per run is only 40 minutes. Compared to the 2-3 hours of the traditional phenol-chloroform method, this product significantly shortens the experimental cycle and improves laboratory efficiency.

This kit uses magnetic bead purification technology, which has three significant advantages over the traditional silica membrane spin column method: First, no centrifugation needed – magnetic separation technology completely eliminates the risk of spin column clogging, particularly suitable for processing high-viscosity saliva samples. Second, excellent automation compatibility – can seamlessly interface with various nucleic acid extraction instruments for high-throughput unattended automated extraction. Third, superior yield and purity – the large surface area of the magnetic beads ensures maximal DNA binding efficiency, and the washing steps thoroughly remove inhibitors, guaranteeing product quality.

The high-purity genomic DNA extracted with this kit can be widely used for real-time qPCR (for gene expression analysis and pathogen detection), next-generation sequencing NGS (for whole-genome sequencing, targeted sequencing, and exome sequencing), gene chips (for SNP genotyping and genome-wide association analysis), STR typing (for forensic individual identification and paternity testing), and molecular cloning (for restriction digestion and ligation and other routine molecular biology operations).

The kit extracts highly intact genomic DNA with fragment sizes up to 15 kb and good integrity, meeting the requirements of experiments demanding large DNA fragments such as NGS sequencing and gene cloning.

Yes. It is perfectly compatible with both pipetting-based and magnetic rod-based automated instruments. With 32T/48T/96T pre-filled specifications, it is suitable for high-throughput automated extraction of large sample batches.

No phenol, chloroform, or other hazardous organic solvents are needed. Purification is accomplished using dedicated magnetic beads and buffer systems, ensuring safe, contamination-free experiments with a simple workflow.

Four specifications are available: standard 96T, pre-filled A-type 32T, pre-filled B-type 48T, and pre-filled C-type 96T. The 32T suits small-batch samples, 48T is suitable for small-to-medium laboratories, and 96T standard and pre-filled versions fit high-throughput experiments and automated workstations.

No cold chain is needed. The kit supports ambient-temperature shipping throughout. For daily storage, simply keep it at 10-30 degrees C room temperature, sealed and protected from light, with relaxed storage and transport conditions.

In addition to conventional dry/preservative-solution oral swabs, it is also suitable for throat swabs, oral mouthwash, and other oral cavity sample types, covering a broad range of sample types.

The purified high-purity DNA can be directly used for qPCR, real-time PCR, gene cloning, NGS high-throughput sequencing, blot hybridization, and various other molecular biology experiments.

With a simplified workflow and no complex pre-treatment required, all experimental steps from sample processing to DNA elution can be completed within 1 hour, saving time and improving efficiency.

Using proprietary nano-magnetic beads and buffer formulation, the extraction efficiency is stable with strong batch-to-batch reproducibility. DNA purity meets standards, is free of impurity contamination, and yields reliable experimental data.

The dried blood spot DNA extraction kit is specifically optimized for dried blood spot samples on filter paper or blood collection cards. Conventional blood DNA extraction kits typically require fresh or frozen anticoagulated blood samples (e.g., EDTA-anticoagulated blood), while the dried blood spot kit effectively handles the DNA-protein crosslinking caused by the drying process and releases DNA encapsulated by blood cell membranes and hemoglobin. The YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit uses a unique lysis buffer system and nano-magnetic bead technology to efficiently extract high-quality genomic DNA from 3-6 dried blood spot punches of 3 mm diameter, making it especially suitable for forensic evidence, newborn screening, and epidemiological cohort studies involving filter paper dried blood spot samples in batch processing.

Yes. The YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit is perfectly compatible with both pipetting-based and magnetic rod-based automated nucleic acid extraction instruments. The product is available in pre-filled plate specifications (CAT#YM26008-A/B/C, corresponding to 32T/48T/96T), which can be directly loaded onto the instrument. With automated instruments, high-throughput extraction of 96 samples can be completed in a single run within 45-60 minutes, yielding PCR-ready high-quality DNA. This greatly improves experimental efficiency and sample processing throughput, and is particularly suitable for large-scale screening and cohort research projects.

The YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit provides high-purity, high-yield DNA extraction results. The DNA purity A260/A280 ratio typically reaches 1.7-1.9, with maximum removal of impurities such as RNA, contaminating proteins, lipids, and PCR inhibitors. For yield, microgram-level genomic DNA can be extracted from 100 microL of dried blood spot sample (approximately 3-6 dried blood spot punches of 3 mm diameter), with yield correlated to the original blood cell count of the sample.

The genomic DNA extracted with the YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit is of high purity and good integrity and is compatible with a variety of downstream molecular biology experiments, including but not limited to: conventional PCR and multiplex PCR amplification; qPCR (Real-time PCR) gene expression analysis; STR typing and capillary electrophoresis analysis; NGS high-throughput sequencing library construction; gene cloning and vector construction; Southern blot hybridization; gene chip hybridization analysis; SNP genotyping detection, etc. The obtained DNA can be directly used for these experiments without additional purification steps.

Yes. The YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit has been specially optimized for aged blood spots and partially degraded samples commonly encountered in forensic scenarios. The kit uses an efficient lysis buffer system that effectively lyses blood cell membranes and nuclear membranes to release DNA fragments. Combined with efficient wash steps, it removes hemoglobin, humic acid, and other impurities that may inhibit PCR reactions. Even for dried blood spot samples stored at room temperature for weeks to months, the kit still yields DNA template of sufficient quality and quantity for STR typing and forensic identification analysis. For severely degraded samples, it is recommended to appropriately increase blood spot input (e.g., 6-8 punches) to improve DNA yield.

Low DNA yield may be caused by the following: Sample issues – insufficient original blood volume in the dried blood spot sample, or prolonged storage leading to severe DNA degradation. Check blood spot sample quality and appropriately increase blood spot input (3-6 punches of 3 mm diameter). Operational issues – magnetic beads not fully mixed before use. Magnetic beads tend to settle during static storage; vortex for at least 30 seconds before use to ensure complete resuspension. Incomplete lysis – reduced Proteinase K activity or insufficient lysis time. Check Proteinase K storage conditions and ensure the lysis step is fully performed. Improper wash buffer preparation – some wash buffers require addition of absolute ethanol before first use as described in the manual. If yield remains consistently low, re-run the experiment strictly following the manual’s procedural steps.

The YALI BIOTECH YALEPIC magnetic bead-based blood spot DNA extraction kit is suitable for dried blood spot samples from various application scenarios: Forensic evidence identification – crime scene bloodstains, mixed stains from sexual assault cases, disaster victim identification. Paternity testing and kinship confirmation – family blood spot samples collected on filter paper blood collection cards. Newborn inherited metabolic disease screening – large-scale screening of newborn heel-prick dried blood spot samples. Clinical pharmacogenomics research – drug metabolism-related gene testing, therapeutic drug concentration monitoring. Epidemiological cohort studies – genetic analysis and biobank construction for large-scale population cohorts. Preclinical toxicokinetics research – drug metabolism analysis of animal dried blood spot samples. Sample types are compatible with various commonly used blood collection filter papers and cards such as Whatman 903, S&S 903, and FTA cards.

This kit is suitable for various blood sample types, including: freshly collected anticoagulated whole blood (EDTA anticoagulant, sodium citrate anticoagulant, heparin anticoagulant), frozen preserved anticoagulated blood samples, blood clots, and buffy coat samples. Regardless of whether the sample is freshly collected or has been long-term frozen stored, high-quality genomic DNA can be obtained. A starting volume of 50 microL to 1 mL of blood sample is recommended for extraction, and the specific starting volume can be adjusted based on downstream experimental needs.

This kit uses unique nano-magnetic bead separation technology and an optimized buffer system. The extracted genomic DNA is of high purity, with OD260/280 ratio between 1.8-2.0 and OD260/230 ratio >1.8, effectively removing PCR inhibitors such as hemoglobin and heparin. DNA fragment integrity can reach 50 kb, and yield is stable and reliable (5-15 microgram of high-quality genomic DNA can be extracted from 200 microL of whole blood). The extraction product fully meets the requirements of downstream experiments with high DNA quality and purity demands, such as next-generation sequencing (NGS) library construction, qPCR, and Southern blot hybridization.

Yes, the YALEPIC MagEVO series kits are available in bottle and pre-filled specifications (32T/48T/96T pre-filled), perfectly compatible with mainstream magnetic bead-based automated nucleic acid extractors, matching instruments such as the YALI BIOTECH YALEPIC Pure 32 Automated Nucleic Acid Extractor CAT#MC-A-32-16. The entire extraction process is based on the magnetic bead separation principle, without the need for centrifugation or vacuum filtration. All steps of lysis, binding, washing, and elution are completed on the automated equipment, greatly reducing manual operational errors and suitable for high-throughput sample processing scenarios.

Using the YALEPIC MagEVO magnetic bead-based blood DNA extraction kit, the manual operation portion is very simple (only requiring sample addition and simple magnetic rack separation steps), and the entire workflow can be completed within 1 hour. If used with an automated nucleic acid extractor, in 96 deep-well plate mode, a single extraction (including lysis, binding, washing, and elution) takes only 35-45 minutes, significantly improving laboratory work efficiency. The entire process does not require toxic organic solvents such as phenol/chloroform, ensuring safe and environmentally friendly operation.

The high-quality genomic DNA extracted with this kit can be widely used in the following molecular biology experiments: qPCR and conventional PCR – for gene expression analysis, pathogen detection, genotyping, etc. Next-generation sequencing (NGS) – library construction, whole genome sequencing, targeted sequencing, metagenomic sequencing. Gene cloning and vector construction – restriction enzyme digestion, ligation and transformation. Southern blot hybridization – gene copy number analysis, transgene detection. Chip detection – gene chip hybridization, SNP genotyping. Restriction enzyme analysis – restriction fragment length polymorphism (RFLP) analysis. The extraction product requires no further purification and can be directly used as template in the above experiments.

The YALEPIC MagEVO magnetic bead-based blood DNA extraction kit supports ambient-temperature shipping (10-30 degrees C) without cold-chain logistics, effectively reducing transportation costs. Upon arrival, store the product at 10-30 degrees C room temperature protected from light; do not freeze. Under proper storage conditions, the product shelf life is 12 months from the date of production. After opening, use as soon as possible and cap the bottles tightly after use to avoid reagent contamination or component evaporation.

This kit uses an optimized low-salt elution buffer in the elution step, minimizing magnetic bead carryover and ensuring that the eluted DNA product does not contain components that inhibit PCR reactions (such as high salt, EDTA carryover, etc.). Additionally, the wash steps of the kit are specially optimized to effectively remove PCR inhibitors such as hemoglobin, heparin, and proteins. The extracted DNA product can be directly used for PCR and qPCR reactions without additional purification steps. If your downstream experiments are highly sensitive to inhibitors, it is recommended to appropriately dilute the DNA template before performing the amplification reaction.

The YALEPIC MagEVO series focuses on blood genomic DNA extraction and is particularly suitable for extracting high-molecular-weight genomic DNA from whole blood samples. If you need to extract viral DNA/RNA or cell-free DNA (cfDNA/cfRNA) from blood, YALI BIOTECH offers dedicated YALEPIC series viral DNA/RNA extraction kits and cell-free DNA extraction kits, respectively optimized for low-abundance pathogen nucleic acids and trace cfDNA samples. Please contact us through our official channels for consultation and purchase.

You can choose the appropriate extraction method based on experimental scale: Manual extraction (low throughput) – only a magnetic rack is needed (can be purchased separately or self-provided), with no other special equipment required. It is recommended to use nuclease-free centrifuge tubes and pipette tips. Automated extraction (high throughput) – a magnetic bead-based automated nucleic acid extractor is required, along with supporting automated consumables such as 96-well deep-well plates and magnetic rod sleeves.

If you encounter low yield or unsatisfactory purity, troubleshoot according to the following steps: Sample issues – check whether the blood sample is fresh and whether the correct anticoagulant (EDTA or sodium citrate recommended) was used, and whether the sample has undergone repeated freeze-thaw. Lysis step – confirm that the lysis buffer has been thoroughly mixed and that lysis time and temperature meet the manual’s requirements. Magnetic bead separation – confirm that the magnetic rack is functioning properly and that magnetic beads have been fully adsorbed and collected. Wash steps – confirm that the wash buffer has been prepared and used as required and that the number of washes is sufficient. Elution step – confirm that the elution buffer has been preheated (65 degrees C preheating recommended) and that the elution time is sufficient (5-10 minutes recommended).

FFPE stands for Formalin-Fixed, Paraffin-Embedded, which is the standard method for long-term preservation of tissue samples in pathology. Massive FFPE biological sample resources have been accumulated over the past decades, containing rich disease information. The core challenges of DNA extraction from FFPE samples are: (1) During formalin fixation, formaldehyde cross-links with DNA and proteins, chemically ‘locking’ the DNA. (2) Long-term room-temperature storage further accelerates DNA degradation and fragmentation. (3) The deparaffinization process, if using organic solvents such as xylene, may damage the sample during paraffin embedding removal. (4) Sample quantity is typically extremely limited and precious. The YALEPIC MagEVO kit effectively addresses these challenges through optimized lysis conditions and de-crosslinking steps, achieving efficient extraction of high-quality DNA from precious FFPE samples.

Yes. The DNA extracted with the YALEPIC MagEVO kit is of high purity and good fragment integrity, fully compatible with NGS library preparation workflows. Test data show that the A260/A280 ratio of the extraction product is between 1.8-2.0, and PCR inhibitors are effectively removed, ensuring library construction efficiency and sequencing data quality for downstream sequencing. It is recommended to use a Qubit fluorometer for accurate quantification before NGS library preparation.

Absolutely. The DNA extracted with the MagEVO kit has removed amplification inhibitors caused by formalin cross-linking, and the extraction product can be directly used for qPCR and Real-time PCR detection. Thanks to the efficient de-crosslinking step, amplification product length can reach 1 kb, providing stable and reliable performance in gene expression quantification and mutation detection.

Not at all. The YALEPIC MagEVO kit uses an innovative non-toxic chemical deparaffinizing agent to replace traditional xylene. The entire extraction process involves no toxic organic solvents, posing no health hazard to laboratory personnel and requiring no special fume hood equipment. This not only ensures laboratory safety but also avoids tissue shrinkage, hardening, and embrittlement that xylene may cause, while aligning with the concept of green and environmentally friendly laboratory construction.

Using the YALEPIC MagEVO kit to extract DNA from FFPE tissue sections, the entire operation workflow takes approximately 30-40 minutes. No overnight incubation is required, saving significant time compared with traditional overnight digestion methods and greatly improving laboratory work efficiency.

This kit can be shipped and stored at 10-30 degrees C ambient temperature, without the need for cold chain or special refrigeration equipment, reducing logistics and storage costs. Do not freeze any kit components. Before use, mix thoroughly. Room-temperature storage shelf life is up to 24 months.

It is recommended to use 5-8 FFPE tissue sections with a thickness of 5-10 microM, with a total section area of approximately 1×1 cm2 and total tissue amount not exceeding 20 mg. Section thickness is recommended to be controlled within 20 microM. For different tissue types, the number of sections can be appropriately adjusted based on tissue cell density.

DNA yield depends on multiple factors, including tissue source, sample storage time, fixation conditions, and section thickness. Under optimized conditions, 2-8 microgram of genomic DNA can be extracted from 5-8 FFPE sections of 5-10 microM thickness. There are differences among tissue types, with tumor tissue typically yielding higher amounts and fibrotic tissue yielding relatively lower amounts.

The following methods are recommended for quality testing: Concentration detection – use a Qubit fluorometer with dsDNA HS assay kit for specific quantification of double-stranded DNA without RNA or protein interference. YALEPIC 1x dsDNA high-sensitivity assay kit CAT#YNQ24091 is available. Purity assessment – use a NanoDrop spectrophotometer to detect the A260/280 ratio, with the ideal range being 1.8-2.0. Integrity analysis – for high-demand applications such as NGS, use a bioanalyzer for DNA fragment distribution analysis.

It is recommended to aliquot and store the purified DNA at -20 degrees C, avoiding repeated freeze-thaw. Under -20 degrees C conditions, it can be stably stored for at least 2 years. For long-term archiving, -80 degrees C ultra-low temperature storage is recommended. Use nuclease-free (DNase/RNase-free) storage tubes and avoid prolonged exposure of DNA solution at room temperature.

The YALEPIC MagEVO kit uses magnetic bead-based purification technology and is fully compatible with mainstream automated nucleic acid extractors on the market. The magnetic bead-based automated workflow can significantly improve throughput and result reproducibility compared with manual operation.

Compatible with plasma, serum, amniotic fluid, and other cell-free body fluids; standard starting sample volume is 0.2-1 mL, compatible with blood samples collected in EDTA conventional blood collection tubes and Streck cfDNA-dedicated stabilizer tubes.

Fully supports automated extraction, compatible with 32-channel and 96-channel mainstream nucleic acid extraction instruments. Optimized pre-loaded reagent versions (Type A / Type B / Type C) are also available, requiring no manual preparation, for direct instrument use.

The purified product has no protein, salt ion, or other inhibitor residues, and can be directly used for qPCR quantification, NGS next-generation sequencing, library construction, non-invasive prenatal testing, tumor liquid biopsy, methylation analysis, and other experiments.

It is not an extraction failure. cfDNA is naturally present at extremely low levels in plasma and is a trace nucleic acid. Nanodrop UV detection has insufficient sensitivity and tends to show lower values. Using the Qubit high-sensitivity fluorometer for concentration detection is recommended for more accurate results.

Fresh plasma is recommended for short-term storage at 4 degrees C after separation; for long-term storage, aliquot and store at -20 degrees C, avoiding repeated freeze-thaw. If blood samples cannot be processed promptly, cfDNA-dedicated stabilizer blood collection tubes are recommended to prevent blood cell lysis releasing genomic DNA contamination.

No cold chain needed; room temperature transport is supported. Store sealed at 10-30 degrees C room temperature in the dark. The shelf life is stable when unopened. Avoid placing in damp, cold, or environments above 35 degrees C.

This kit is specifically optimized for short-fragment cfDNA. The proprietary nano-magnetic beads efficiently capture small-fragment nucleic acids, with uniform fragment coverage and no selection bias, maximally preserving the original nucleic acid information of the sample.

The standard YM26004 is a standard reagent that can be self-configured for manual or instrument use; YM26004-A/B/C are pre-loaded versions, pre-dispensed to match instrument consumables, ready to use, eliminating preparation steps, suitable for high-throughput batch experiments. Choose Type A for 32T, Type B for 48T, and Type C for 96T.

The entire process requires no phenol, chloroform, beta-mercaptoethanol, or other toxic organic solvents. Purification relies on magnetic bead adsorption plus proprietary wash buffer, making the operation safe and the experimental environment more friendly.

Eluted cfDNA can be stored at 4 degrees C for 1-3 days short-term; for long-term storage, aliquot and store at -20 degrees C or -80 degrees C, avoiding repeated freeze-thaw to prevent nucleic acid degradation that could affect downstream experimental results.

It is primarily suitable for fresh animal tissues (mouse tail, muscle, viscera, etc., 10-30 mg) and routine cultured cells (<=1×10^6 cells). It is not suitable for paraffin-fixed tissues or blood samples. For other sample types, please consult customer service for recommendations.

Yes. The genomic DNA extracted with this kit has intact fragments and high purity, with a 260/280 ratio stably between 1.8-2.0, free of impurity contamination, and can be directly used for metagenomic library construction, whole genome sequencing, qPCR, and other high-end experiments.

Yes. It is compatible with mainstream pipetting-based automated nucleic acid extractors and magnetic rod-based automated extraction workstations. The 32T/48T/96T pre-filled models can be directly loaded onto instruments without manual reagent preparation, suitable for batch sample testing.

No. The product supports ambient-temperature shipping. For daily storage, simply keep it at 10-30 degrees C room temperature protected from light, without freezing or refrigeration, with relaxed storage conditions.

The operation is extremely simple. Samples are directly lysed without cumbersome incubation, and the entire DNA extraction and purification can be completed within 1 hour. Even novices can quickly get started without professional laboratory training.

Four specifications are available: 32T pre-filled A, 48T pre-filled B, 96T standard/pre-filled C. Small-batch purchasing is supported, and bulk customization quotes are also available, meeting the throughput needs of different laboratories.

The most likely causes are excessive sample input, incomplete lysis, or improper wash step operation. Strictly follow the manual to control tissue sample input at 10-30 mg, vortex thoroughly for lysis, and complete the wash steps properly to avoid contamination issues.

Compared with spin column methods, the magnetic bead method is more compatible with automated high-throughput operations with no risk of column clogging. No toxic phenol/chloroform reagents are needed, making it safer. It requires less time and provides better DNA recovery and integrity, suitable for routine extraction of large sample batches.

This kit recommends processing 200 microL of anticoagulated whole blood (EDTA, sodium citrate, etc.) per spin column. It can also be used for buffy coat or bone marrow samples. If white blood cell count is very low (e.g., severe leukopenia patients), sample volume can be increased to 300 microL with repeated binding steps to ensure recovery.

The entire blood genomic DNA extraction process typically takes 30-40 minutes. Steps include: sample lysis (10 min), binding (2 min), two washes (1 min centrifugation each), membrane drying (1 min), and elution (1 min). For maximum yield, pre-warm elution buffer to 60 degrees C and incubate 5 minutes before centrifugation – total time ~45 minutes with 10-15% yield improvement.

Absolutely. Extracted genomic DNA typically has OD260/280 of 1.7-1.9, free of heme and protein residues, and contains no organic solvents. It can be directly used as template for routine PCR, qPCR, multiplex PCR, NGS library construction, methylation analysis, and other downstream applications. For demanding digital PCR, dilute with TE buffer to avoid trace salt ions affecting droplet generation.

Common causes and solutions: (1) Low white blood cell count – increase blood volume or enrich leukocyte layer by centrifugation before lysis; (2) Frozen blood not fully mixed – thaw slowly on ice and invert to mix; (3) Insufficient proteinase K lysis – verify buffer not expired, maintain 56 degrees C, extend to 20 minutes; (4) Elution buffer missed membrane center – apply vertically to membrane center, incubate 2-5 minutes at room temperature before centrifugation. If still no improvement, use fluorometric detection for trace DNA.

Heparin-anticoagulated whole blood can be used, but heparin has potential PCR inhibitory effects. We recommend washing cells 1-2 times with PBS during lysis, or adding an extra Buffer AW2 wash to remove residual heparin. Check OD260/230 ratio >1.8 after extraction. If PCR inhibition persists, re-purify DNA by 3M sodium acetate/ethanol precipitation, or use a heparin-tolerant polymerase.

Column-based blood DNA extraction kits have clear advantages in cost, ease of operation, and DNA purity stability. Compared to magnetic bead methods, column methods require no magnetic stand – only a standard benchtop centrifuge. Sample processing volume is flexible, and elution volume can be as low as 50 microL, yielding higher DNA concentration suitable for limited-sample experiments. For most research and clinical routine testing, column methods are fully sufficient; magnetic bead methods only have throughput advantages for fully automated large-batch extraction.

Purified genomic DNA should be aliquoted and stored at -20 degrees C, stable for 2+ years. For long-term storage, use -80 degrees C. Avoid repeated freeze-thaw as multiple cycles cause mechanical shearing of large DNA fragments, reducing average length. Keep freeze-thaw cycles per tube to 3 or fewer.

Standard adaptation is for 1-5 mL of overnight culture. For low-copy plasmids, the culture volume can be increased to within 10 mL by proportionally scaling up the volumes of Buffer PA, PB, and ER for normal extraction.

The entire process takes only 35 minutes to complete plasmid extraction, using a modified alkaline lysis method combined with silica membrane binding technology. No toxic reagents such as phenol or chloroform are required. The steps are simplified and even novices can quickly get started.

The endotoxin carryover of the product is <1 EU/microgram, meeting professional transfection-grade standards, with no toxicity to eukaryotic cells, and can be directly used for various sensitive cell transfection experiments.

The maximum plasmid DNA yield per sample can reach up to 45 microgram, with high purity and no RNA or protein carryover, fully meeting the requirements of downstream experiments such as gene sequencing, restriction enzyme digestion, PCR, and in vitro transcription.

It supports ambient-temperature shipping. For daily storage, it only needs to be kept sealed at 10-30 degrees C room temperature; -20 degrees C cold storage is not required, making storage and transport more convenient.

A pre-mixed proprietary color indicator allows intuitive judgment of whether cell lysis and neutralization reactions are complete through liquid color change, achieving visualized operation and effectively avoiding extraction failure due to improper experimental handling.

The 50T specification can complete 50 standard 1-5 mL culture plasmid extraction experiments, with ample reagent ratios and high cost-effectiveness, suitable for routine batch experiments in laboratories.

Endotoxin-free plasmid purified with this kit has high purity and no impurity contamination, can be routinely aliquoted and stored long-term at -20 degrees C, resists degradation, and maintains good reproducibility of experiments.

This kit is universally applicable to all routine research strains of Gram-negative and Gram-positive bacteria, meeting the genomic DNA extraction needs of most common bacterial samples in laboratories.

It does not affect the basic extraction performance. The kit includes an optimized buffer system that naturally removes most RNA impurities. If complete removal of RNA residue is required, standard RNase A can be added separately, providing flexibility to meet different experimental standards.

No. Using an improved silica membrane spin column method, the entire process avoids harmful organic solvents such as phenol and chloroform, and eliminates ethanol precipitation steps. The experiment is safe, non-polluting, and the workflow is more streamlined.

The standard single preparation accommodates 10^6 to 10^8 bacterial cells, with a maximum capacity of 2.0 x 10^9 Gram-negative or Gram-positive bacterial cells, meeting the extraction needs of routine and medium-to-high concentration bacterial culture samples.

The extracted high-purity, intact genomic DNA can be directly used for restriction digestion, conventional PCR amplification, real-time qPCR, blot hybridization, gene library construction, bacterial strain sequencing, and other molecular biology experiments.

Following the standard protocol, the complete DNA extraction workflow including lysis, binding, washing, and elution for multiple bacterial samples can be completed within 1 hour, suitable for batch sample processing.

Room temperature transport is supported without cold chain requirements. For daily storage, place in a dry, dark environment at 10-30 degrees C room temperature. Storage conditions are flexible and the kit is not prone to failure.

The kit’s proprietary buffer system maximally removes contaminating proteins, lipids, and inhibitory impurities. Strictly follow the protocol to control the bacterial sample input amount and ensure thorough cell lysis to guarantee high DNA purity without experimental inhibition.

No. This kit is specifically designed for bacterial cell wall lysis and is not compatible with eukaryotic microorganisms such as yeast and fungi. Such samples require a dedicated fungal genomic DNA extraction kit.

DNA extraction from polysaccharide- and polyphenol-rich plant samples is difficult for two main reasons: First, polysaccharides co-precipitate with DNA, forming insoluble gelatinous impurities that make the DNA solution viscous, affecting nucleic acid release and subsequent operations. Second, polyphenols are easily oxidized to quinones after cell disruption, covalently binding with nucleic acids and causing the extracted DNA solution to turn brown, while also inhibiting PCR enzyme activity and affecting downstream amplification. This kit uses a unique precipitation solution and silica membrane technology to effectively precipitate and remove proteins, polysaccharides, and phenolic impurities, yielding high-purity, stable-quality genomic DNA.

This kit is suitable for various plant tissues rich in polysaccharides and polyphenols. Typical samples include: cotton, pine, and ginkgo; banana and grape fruit flesh; wheat and peanut seeds; mushroom fungi; and Rosaceae family plants with high polysaccharide and polyphenol content (such as apple, pear, peach, etc.). Additionally, it is also suitable for DNA extraction from routine plant samples, with an extremely broad application range.

Low yield may result from the following: (1) Poor sample quality: Experimental material is not fresh or has undergone repeated freeze-thaw, causing DNA degradation. Use fresh material whenever possible, and avoid repeated freeze-thaw of low-temperature stored materials. (2) Insufficient lysis: Incomplete plant grinding or inadequate cell lysis. Liquid nitrogen grinding should be thorough to a fine powder, vortex thoroughly after adding lysis buffer, and lysis time can be appropriately extended. (3) Improper sample amount: Too little sample (insufficient total DNA) or too much sample (incomplete lysis). Follow the recommended amounts: fresh plant sample <=100 mg, dry plant sample <=20 mg. (4) Elution issues: Elution buffer not pre-warmed or elution volume too small. Pre-warm YGE Buffer to 65 degrees C before elution, or increase the number of elution steps.

DNA degradation is typically caused by: (1) Repeated freeze-thaw of material: Samples should avoid repeated freeze-thaw, otherwise the extracted DNA fragments will be small and yield will decrease. (2) Overly vigorous operation: After liquid nitrogen grinding or tissue homogenization, subsequent operations should be gentle, avoiding vigorous pipetting or vortexing. DNA elution buffer should also avoid repeated freeze-thaw. (3) RNase contamination: RNase A dosage and treatment time during extraction should be standardized, avoiding exogenous RNase contamination. Using separate electrophoresis tanks for DNA and RNA is recommended.

The most common cause of spin column clogging is insufficient sample grinding/lysis or large amounts of viscous polysaccharide substances in the lysate. Solutions include: (1) Check if the lysate is clear, remove overly viscous insoluble material before loading onto the column. (2) Appropriately reduce the initial sample amount. For samples with particularly high polysaccharide content, use the lower end of the recommended range in the instructions. (3) Ensure YPC Buffer and Wash Buffer GB have been supplemented with anhydrous ethanol as required before first use. (4) All centrifugation steps should be performed at room temperature; low centrifugation temperature may result in incomplete impurity precipitation.

Colored DNA solution is usually caused by excessive sample amount or incomplete removal of polyphenolic compounds from plant tissue. Polyphenols oxidize to form brown quinone compounds that covalently bind with nucleic acids. Solutions: reduce the initial sample amount according to the recommended dosage in the instructions; appropriately increase the number of wash steps after lysis.

A low A260/A280 ratio (less than 1.8) typically indicates protein or polyphenol residue. Improvement methods: (1) Ensure thorough centrifugation after lysis, and avoid drawing up the pellet when transferring the supernatant to a new tube. (2) Strictly follow the protocol, ensure Wash Buffer GB has been supplemented with anhydrous ethanol, and that wash steps are thorough. (3) After washing, centrifuge the empty column for 1-2 minutes to ensure complete ethanol evaporation before elution.

A low A260/A230 ratio (less than 2.0) typically indicates polysaccharide or salt residue. DNA solutions with polysaccharide contamination often appear viscous, difficult to dissolve and pipette, and can inhibit PCR, reverse transcription, and ligation reactions. Improvement methods: (1) Ensure YPC Buffer has been supplemented with anhydrous ethanol as required, add at 1.5x the sample volume, mix thoroughly and let stand for an appropriate time. (2) Wash steps cannot be omitted; Wash Buffer GB should thoroughly wash the spin column. (3) For samples with extremely high polysaccharide content (such as certain fruit flesh), appropriately reduce the initial sample amount.

Absolutely not. This kit uses a highly efficient, nucleic acid-specific binding spin column and a unique buffer system, requiring no phenol/chloroform or other organic reagents throughout the process. Plant genomic DNA extraction can be completed within 30 minutes, making the operation safe and convenient. Compared to the traditional CTAB method, this kit eliminates exposure to chloroform and other toxic chemicals, and also avoids the polysaccharide co-precipitation problem associated with traditional ethanol or isopropanol DNA precipitation.

The DNA purified with this kit can be directly used for various downstream experiments, including but not limited to PCR amplification, real-time qPCR, restriction enzyme digestion, library construction, Southern blot, molecular markers (such as SSR, AFLP, RAPD, etc.), and next-generation sequencing (NGS). The extracted DNA has high purity and good integrity, with PCR and other enzymatic reaction inhibitors successfully removed, and can be used directly without additional purification.

This kit is transported at room temperature and can be stored at 10-30 degrees C room temperature. The shelf life is one year. Before use, check YPA Buffer and YPB Buffer for crystallization or precipitate. If crystals or precipitate are present, redissolve YPA and YPB Buffer in a 56 degrees C water bath before use. YPC Buffer and Wash Buffer GB must be supplemented with anhydrous ethanol according to the reagent bottle label before first use.

Yes. For high-water-content samples (such as strawberry, watermelon, tomato fruit flesh, etc.), the high water content in plant tissue results in relatively lower DNA content, and appropriately increasing the sample amount is recommended. However, the sample processing amount should not exceed the kit’s capacity limit, otherwise incomplete sample lysis will occur, affecting DNA yield and purity. It is recommended to start with 100 mg and gradually increase if yield is insufficient.

DNA yield varies by sample type and amount. Typically: 3-6 micrograms from 200 microL healthy whole blood; 10-30 micrograms from 25 mg animal tissue (e.g., liver); 15-40 micrograms from 5 x 10^6 cultured cells (e.g., HEK293). We recommend reviewing literature for target sample DNA content before experiments.

This is a universal kit with an optimized lysis buffer system suitable for various biological samples. Besides tissues, blood, and cells, it can effectively process bacteria (E. coli) and fungi (yeast). For plant tissues, FFPE (formalin-fixed paraffin-embedded) samples, and other special cases, it is not recommended.

DNA degradation relates to sample handling and operation. First, use freshly collected or properly frozen samples, avoiding repeated freeze-thaw (a major cause of nuclease-mediated DNA degradation). Second, insufficient lysis affects DNA integrity – ensure thorough tissue grinding and even mixing with lysis buffer. After extraction, aliquot DNA and store at -20 degrees C to avoid repeated freeze-thaw.

Common causes: Sample itself – low DNA content (low white blood cell count, over-cultured bacteria) or degradation from improper storage. Insufficient lysis – tissue pieces too large, proteinase K inactivated or insufficient incubation. Extend 56 degrees C incubation (overnight for difficult samples) and check proteinase K activity. Reagent issues – forgot to add ethanol to wash buffer (a common beginner mistake). Elution issues – pre-warm elution buffer to 60-70 degrees C, apply to membrane center, incubate 1-5 minutes before centrifugation, or perform double elution.

Purity is critical for downstream experiments: Low OD260/280 (below 1.9) indicates RNA residue. Add 4 microL RNase A (100 mg/mL) before proteinase K, incubate at room temperature for 5 minutes. DNA difficult to dissolve may indicate over-drying of precipitate. Air dry only, no more than 5 minutes, avoid vacuum drying.

Mainly due to inhibitor residues such as ethanol or salt ions in purified DNA. Solutions: spin empty column at max speed for 2-3 minutes after washing to thoroughly dry the column; ensure ethanol has been added to solutions as indicated on bottles. If DNA is impure, increase wash cycles or add BSA to PCR at 0.1 microgram/microL to mitigate inhibition.

Column clogging is typically caused by insufficient lysis or excessive sample input. Try: extend lysis time and increase inversion mixing; reduce starting sample amount; ensure lysate is completely clear before centrifugation.

The kit is very stable and can be stored and transported at room temperature (15-25 degrees C) with a shelf life of 18 months. Proteinase K (if in powder or solution form) should be aliquoted and frozen per protocol to avoid repeated freeze-thaw. No special cold storage needed, providing great convenience for daily laboratory use.

This kit is standardly suitable for 5-15 mL of overnight E. coli culture. For high-copy plasmids, 5 mL of culture can meet the 100 microgram yield requirement. For low-copy plasmids or plasmids with large backbone (>10 kb), it is recommended to use 10-15 mL of culture to achieve more ideal yield.

The endotoxin carryover of plasmid DNA extracted with this kit is <=1 EU/microgram, meeting transfection-grade plasmid standards. This low endotoxin level fully meets the transfection requirements of various common cell lines such as HEK293, CHO, and HeLa, and is compatible with various transfection reagents such as Lipofectamine 3000, PEI, and the YALEPIC LFDR series, significantly reducing the negative impact of endotoxin on transfection efficiency and cell viability.

From culture collection to obtaining purified plasmid DNA, the entire operation workflow takes approximately 25 minutes. Optimized procedural steps and an efficient binding membrane system greatly shorten the time cost of traditional plasmid extraction. The visual blue indicator also helps users quickly determine whether lysis and neutralization are complete, further reducing operation waiting and re-verification time.

The blue indicator is a pre-mixed component of this kit. Its main function is to visually indicate whether lysis and neutralization steps are complete through color change – the solution turns blue when lysis is sufficient, and the blue color fades or becomes colorless/light when neutralization is complete. This design effectively avoids reduced plasmid yield and purity issues caused by insufficient lysis or over-neutralization, making it especially suitable for novice users or high-throughput operation scenarios.

Plasmid DNA extracted with this kit has extremely low endotoxin carryover and minimal salt carryover, and is perfectly compatible with the following downstream experiments: Cell transfection (recommended, suitable for various endotoxin-sensitive cell lines); Restriction enzyme digestion; PCR amplification and qPCR quantification; Sanger sequencing and NGS library construction; In vitro transcription (e.g., mRNA synthesis template); Competent cell transformation; Ligation and cloning.

This kit employs a dual-effect endotoxin removal mechanism: First, large-molecule endotoxin complexes are physically removed through a filtration column after cell lysis. Then, a specialized endotoxin removal solution system performs chemical clearance. The silica membrane specifically binds plasmid DNA while effectively excluding impurities such as endotoxin, protein, and genomic DNA, ultimately yielding high-purity endotoxin-free plasmid DNA.

Yes, but it is recommended to appropriately increase the culture volume. For low-copy plasmids (e.g., pACYC and pSC101 backbones) or large plasmids >10 kb, 10-15 mL of overnight culture is recommended. The lysis time can also be appropriately extended (approximately 1-2 minutes) to ensure complete cell lysis. During elution, using Elution Buffer preheated to 65 degrees C helps improve the elution efficiency of large plasmids.

Compared with imported brands, the YALEPIC kit offers equivalent performance quality at a more cost-effective price. Compared with domestic brands, this kit delivers equivalent yield (100 microgram) and endotoxin standard (<=1 EU/microgram) for the same 5-15 mL culture processing volume, while the visual blue indicator design is a core differentiating highlight. Localized production and fast logistics response also provide domestic users with higher service efficiency.

This kit supports ambient-temperature shipping (10-30 degrees C). Upon arrival, it can be stored directly at room temperature without refrigeration. Do not freeze any components, as freezing may cause reagent performance degradation. The product shelf life is 12 months (subject to the product label). Use within the shelf life and store properly after opening according to the instructions.

Common causes and solutions are as follows: High endotoxin – incomplete neutralization after lysis, buffer not mixed until no localized shadow remains. Thoroughly mix by inverting to ensure complete neutralization before filtration. Insufficient yield – insufficient culture volume or poor bacterial growth state. Use freshly overnight-cultured bacteria with OD600 recommended at 2.0-4.0. Poor purity – insufficient wash steps. Wash twice as described in the manual and ensure wash buffer has completely drained before elution. Difficult elution of large plasmids – preheat elution buffer to 65 degrees C before use, which significantly improves elution efficiency.

This kit processes 100-300 ml of fresh overnight E. coli culture per preparation. For high-copy plasmids such as pUC series, 300 ml culture yields 2-3 mg plasmid DNA. For low-copy plasmids such as pBR322, 300 ml culture yields approximately 500-800 micrograms. Actual yield depends on plasmid copy number, host strain, and culture conditions.

Endotoxin (lipopolysaccharide, LPS) is a component of Gram-negative bacterial cell walls and easily remains during plasmid extraction. Endotoxin can: (1) significantly reduce transfection efficiency in sensitive cells; (2) activate immune cells causing non-specific reactions and false positives; (3) cause cytotoxicity and cell apoptosis; (4) trigger fever and endotoxic shock in animal experiments. Therefore, plasmids used for cell transfection and animal experiments must have endotoxin controlled to very low levels.

Plasmid DNA extracted with this kit has endotoxin residual levels below 0.1 EU/microgram, with endotoxin removal rate exceeding 99%. This level meets the requirements for most mammalian cell transfection and mouse tail vein injection experiments, and satisfies NMPA standards for plasmid endotoxin control in gene therapy applications.

The kit contains a pre-mixed blue indicator component. When lysis buffer is added to the bacterial suspension, the solution changes from turbid to clear blue, indicating complete bacterial cell wall lysis and full plasmid release. When neutralization buffer is added, the solution changes from blue to colorless with white flocculent precipitate, indicating complete neutralization and precipitation of proteins and genomic DNA. The color change provides visual confirmation of each step, effectively avoiding quality issues from insufficient or excessive lysis.

If precipitate appears in Buffer PA, Buffer PB, or Buffer ER during storage, this is normal, especially in low winter temperatures. Before use, place the reagent bottle in a 37 degrees C water bath for 10-15 minutes and mix thoroughly by inversion. Once precipitate is completely dissolved, use normally. Note: after adding RNase A to Buffer PA, store at 4 degrees C and use within 6 months.

Low yield is usually related to: (1) Culture time too long (>16 hours), plasmid loss or degradation; (2) Low OD600 (<2.0), insufficient bacteria; (3) Low-copy plasmid (pBR322, pACYC) copy number limitations; (4) Insufficient lysis, incomplete plasmid release. Recommendations: for high-copy plasmids use 100-150 ml culture, culture 12-16 hours, collect at OD600 2.0-4.0; for low-copy plasmids use 300 ml culture directly.

This kit uses silica membrane adsorption technology. For plasmids larger than 20 kb, silica membrane adsorption efficiency and yield decrease, and column processing may cause shear damage to large plasmids. For BAC/PAC and other large plasmids, we recommend ion exchange or solution-based kits.

High post-transfection cell death may result from multiple factors: (1) Excessive endotoxin residue – endotoxin is directly cytotoxic; (2) Transfection reagent toxicity; (3) Ethanol or isopropanol residue in plasmid solution; (4) Insufficient plasmid purity (A260/280 outside 1.8-2.0 range). First check plasmid A260/280 ratio and endotoxin level, and ensure endotoxin-free plasmid is used for transfection.

Yes. All components can be transported and stored at room temperature (15-30 degrees C), greatly reducing transport and storage costs. In dry room conditions, the kit shelf life is 18 months. However, separately packaged RNase A can be stored longer at -20 degrees C; after adding RNase A to Buffer PA, store at 4 degrees C and use within 6 months.

The high-purity, low-endotoxin plasmid DNA is suitable for: mammalian cell transfection (HEK293, CHO, Huh7, etc.), CRISPR-Cas9 gene editing, lentivirus/AAV packaging, animal in vivo injection, restriction digestion, PCR amplification, Sanger sequencing, ligation transformation, in vitro transcription, and cell-free expression systems. For clinical research or gene therapy development, additional endotoxin testing is recommended.

Key differences: (1) Visual operation – lysis and neutralization buffers contain blue indicators, more user-friendly for beginners; (2) Time efficiency – 45 minutes for the full workflow; (3) Cost-effectiveness – YC47013-10 specification list price 650 CNY/10 preps, offering price advantages over similar products, with even greater advantages during promotions. We recommend choosing based on specific experimental needs, plasmid type, and budget.

The kit provides all components except common equipment and reagents. Users need: (1) Centrifuge accommodating 50 ml tubes (max force >=12,000 x g); (2) 37 degrees C shaking incubator; (3) Anhydrous ethanol (for wash buffer preparation); (4) 100-300 ml bacterial culture flasks and media; (5) Microcentrifuge tubes (1.5/2 ml); (6) Microvolume spectrophotometer. No phenol, chloroform, or other organic solvents needed.

It can stably purify and recover all DNA fragments in the 100 bp to 20 kb range, covering mainstream molecular biology experimental fragment sizes.

The entire process uses a rapid purification workflow, requiring only 15 minutes to complete binding, washing, and elution operations, making it highly efficient and time-saving.

Fully compatible, suitable for all conventional concentrations of TAE and TBE agarose gels with no system limitations.

Each spin column can bind up to 10 microgram of DNA, providing ample loading capacity to meet high-concentration sample purification needs.

Yes. Purified DNA has high purity, no impurities, and good integrity, and can be directly used for sequencing, ligation and transformation, in vitro transcription, and various other downstream experiments.

Under standard experimental conditions, DNA recovery rate can reach up to 80%, significantly higher than ordinary purification kits, with greater experimental tolerance.

It supports room-temperature shipping without cold chain. For daily storage, it can be kept sealed at 10-30 degrees C room temperature, making storage convenient.

It can be used not only for agarose gel DNA recovery, but also for purifying crude DNA from PCR products, restriction digestion, ligation, probe labeling, and various enzyme reaction systems.

Yes. The YALEPIC MagEVO magnetic bead-based PCR product/agarose gel DNA recovery kit is specially designed as a dual-use kit – it is suitable both for recovering target DNA fragments from TAE/TBE agarose gels (gel recovery mode) and for directly purifying DNA from PCR reaction systems, restriction digestion systems, ligation reaction systems, and other solutions (purification mode). Recovery efficiency in both modes exceeds 85%, significantly reducing laboratory kit procurement costs and inventory pressure.

The core differences lie in the solid-liquid separation method and automation compatibility: Spin column method – relies on high-speed centrifugation to bind DNA to silica membranes, requiring multiple centrifugation steps with time-consuming manual operation, and is difficult to adapt for high-throughput automated equipment. Magnetic bead method (this product) – uses rapid response of nano-magnetic beads in a magnetic field for solid-liquid separation, requiring no centrifuge, with simpler operation, and can easily integrate with liquid handling workstations, magnetic rod automated extraction systems, and 96-channel automated platforms, making it especially suitable for high-throughput, large-sample-volume laboratory scenarios.

This kit can efficiently recover DNA fragments above 80 bp, with recovery efficiencies for different fragment sizes as follows: 50-80 bp: approximately 50-60%; 80 bp-10 kb: >=85%; 10-20 kb: approximately 60-75%. In gel recovery mode, it is recommended to cut gel bands quickly to minimize UV damage to DNA and avoid prolonged exposure that reduces recovery efficiency.

Yes. This product is specially designed for use with automated workstations or magnetic rod automated magnetic bead extraction systems, compatible with mainstream automated extraction platforms (e.g., KingFisher Flex, Tecan, Hamilton, YALEPIC, etc.). It is available in 96T standard and 32T/48T/96T pre-filled plate versions. The pre-filled version has components pre-dispensed into 96-well deep-well plates, ready to use out of the box, significantly reducing manual preparation time. Whether for batch gel recovery in clone screening, PCR product purification in NGS library construction, or genotyping sample preparation in high-throughput screening, fully automated efficient operation can be achieved.

Stability is very reliable. This kit supports room-temperature shipping and storage at 10-30 degrees C without cold chain. Under room temperature, protected from light, and sealed conditions, the shelf life is up to 18 months. All buffer components have undergone rigorous room-temperature stability validation; even during 7-day summer shipping, performance stability is guaranteed. The room-temperature storage feature makes it especially suitable for labs with limited cold chain conditions, such as primary labs, field sampling bases, and third-party testing facilities.

The purified DNA can be directly used for a variety of downstream molecular biology experiments, including Sanger sequencing, next-generation sequencing (NGS) library construction, PCR/qPCR amplification, restriction enzyme digestion (restriction enzymes/ligases), TA cloning/homologous recombination, DNA labeling and probe preparation, in vitro transcription, and microarray analysis.

RNA degradation is typically caused by the following: Improper sample preservation – animal tissues not frozen or placed in lysis buffer promptly after excision, leading to activation of endogenous RNases. It is recommended to snap-freeze in liquid nitrogen immediately after sampling or directly homogenize in the lysis buffer provided with the kit. RNase contamination – tips, tubes, pipettes, and work surfaces used during the experiment may introduce exogenous RNase. Use RNase-free certified consumables, wear gloves and change them frequently, and prepare reagents with DEPC-treated water. Repeated freeze-thaw of samples – multiple freeze-thaw cycles destroy RNA integrity. Aliquot samples for storage and avoid repeated freeze-thaw. Vigorous operation – excessive vortexing or pipetting may cause mechanical RNA shearing. Mix gently by flicking after lysis and pipette gently.

Low RNA yield can be investigated and optimized from the following aspects: Insufficient sample input – confirm that animal tissue usage is within 15-25 mg and cultured cells within 1×10^6 to 1×10^7. Too little sample will result in low total RNA. Incomplete lysis – for animal tissue, grind to powder in liquid nitrogen before adding lysis buffer, or use an electric homogenizer for thorough homogenization. For cultured cells, pipette thoroughly after adding lysis buffer until cells are completely lysed with no visible clumps. Magnetic bead wash loss – during magnetic separation washing, ensure beads are fully adsorbed by the magnetic rack before discarding the supernatant to avoid aspirating beads. Carefully remove supernatant with a pipette without disturbing the beads. Elution condition optimization – after adding elution buffer, incubate at room temperature for 2-5 minutes. If needed, heat at 55-65 degrees C for 2-3 minutes to enhance elution efficiency. A second elution may be performed if necessary to improve recovery.

The A260/A280 ratio is an important indicator for assessing RNA purity: Ratio below 1.9 – usually indicates protein contamination. Ensure thorough lysis, add an extra wash step, and verify lysis buffer adequately covers all sample material. Ratio above 2.1 – usually indicates RNA degradation or residual salt ions. Recommendations: follow the RNA degradation solutions in the previous question; ensure magnetic beads are adequately dried after washing (briefly centrifuge and aspirate residual wash buffer with a pipette) to avoid ethanol residue affecting the reading. Ideally, high-quality RNA should have an A260/A280 ratio between 1.9 and 2.1.

The MagEVO animal cell/tissue total RNA extraction kit incorporates an on-column/on-bead DNase I digestion step that effectively removes residual genomic DNA. Specific recommendations: DNase I digestion – follow the protocol: after magnetic beads bind RNA, add DNase I reaction mix for on-column/on-bead digestion, ensuring sufficient digestion time (typically 15 minutes at room temperature). DNase I inactivation – wash thoroughly after digestion to remove DNase I and its reaction products, avoiding inhibition of downstream reverse transcriptase. Primer design strategy – if the experiment is extremely sensitive to DNA carryover (e.g., detecting low-abundance genes or limited intron-spanning primer design), design exon-spanning primers or use DNase I-treated RNA as template with a ‘No-RT’ (no reverse transcriptase) control to monitor DNA carryover.

MagEVO pre-filled reagent plates provide a ready-to-use, convenient experience: Product format – pre-filled models A/B/C are 32T/48T/96T pre-dispensed plate reagents. Magnetic beads, lysis buffer, wash buffer, and elution buffer are all pre-arranged in a 96-well plate per the protocol. Users only need to add pre-treated samples before loading onto the instrument. Compatible instruments – this product has been extensively validated and is compatible with mainstream automated nucleic acid extractors, including but not limited to: YALI BIOTECH YALEPIC Pure series automated extractors, Thermo Scientific KingFisher series, and magnetic rod-based extractors from Tianlong, Bioer, and Aosheng. Contact YALI BIOTECH technical support for specific run programs. Key operational points – before using the pre-filled plate, remove the sealing film and check that reagent volumes in each well are intact. Transfer lysed samples to the sample wells and run according to the instrument program. After the run, collect the RNA solution from the elution wells, aliquot and store at -80 degrees C, or use directly for downstream experiments.

Compared with the traditional TRIzol/chloroform method, the MagEVO magnetic bead method offers the following significant advantages: Experimental time – 40 minutes vs. 60 minutes. Organic solvent use – no toxic reagents such as chloroform or isopropanol needed throughout. Operation steps – simplified magnetic separation vs. multiple centrifugation and phase separation steps. Automation compatibility – supports automated extractors for high-throughput vs. difficult to automate. Nucleic acid purity – no organic solvent residue risk, high purity. RNase protection – closed-system operation, RNA is more stable vs. higher exposure risk during the process. Therefore, for laboratories pursuing operational efficiency, experimental safety, and high-throughput processing, the MagEVO magnetic bead-based RNA extraction kit is the more ideal choice.

Different kit components have different storage requirements. Please strictly follow the instructions below: Components stored at -20 degrees C – DNase I and 10x DNase Buffer. After arrival under low-temperature shipping, if not used immediately, store at -20 degrees C. After dissolving DNase I, aliquot into single-use volumes to avoid repeated freeze-thaw which reduces enzyme activity. Components stored at room temperature – magnetic bead suspension, lysis buffer, wash buffer, elution buffer, and all other components can be stored long-term at 10-30 degrees C. Avoid high temperatures (>40 degrees C) or direct sunlight. Special note – after opening the kit, store components separately according to the above conditions and use within the labeled expiration date. If long-unused DNase I shows precipitation or activity decline, replace with a new batch.

The MagEVO animal cell/tissue total RNA extraction kit delivers good extraction results for routine tissue samples. For high-fat-content special samples, the following optimization measures are recommended: Increase lysis buffer volume – lipid components in adipose tissue may interfere with nucleic acid binding efficiency of magnetic beads. For 15-25 mg adipose tissue, increase lysis buffer volume to 500-600 microL (vs. 400 microL standard). Strengthen lysis operations – adipose tissue is recommended to be thoroughly ground to powder in liquid nitrogen, or use a handheld homogenizer in lysis buffer at high speed for 30-60 seconds to ensure complete tissue dispersion. Increase wash steps – after magnetic separation, add an extra wash step with Wash Buffer I to more thoroughly remove residual lipid components. Ensure lysis buffer fully infiltrates – ensure lysis buffer completely covers the tissue powder, mix thoroughly and lyse at room temperature for 5-10 minutes. If needed, heat at 65 degrees C for 5 minutes to assist lysis.

Next-generation sequencing has high requirements for RNA starting quality, mainly in the following aspects: Purity requirements – A260/A280 ratio should be between 1.9-2.1, A260/A230 ratio >=2.0, free of guanidine salts, ethanol, detergent residues, etc. RNA extracted with the MagEVO kit, after stringent washing, is free of these contaminants and fully meets NGS library construction requirements. Integrity requirements – RIN (RNA Integrity Number) should be >=8, with 28S band brightness approximately twice that of 18S band on electrophoresis. The RIN value of RNA extracted with MagEVO, tested by Agilent Bioanalyzer, can reach above 8.5. No DNA carryover – trace DNA carryover may be erroneously counted in transcript quantification results. This product includes DNase I digestion, effectively eliminating gDNA contamination. Recommended input – NGS library construction typically requires 100 ng to 1 microgram of total RNA, corresponding to the cell/tissue input range recommended for this product. Adjust sample starting amount according to specific library preparation kit requirements.

The superparamagnetic nano-magnetic beads used in the MagEVO series have undergone special surface modification and suspension formulation optimization, with the following characteristics: Long-term stability – under recommended 10-30 degrees C room-temperature storage, the magnetic bead suspension is stable for over 12 months. Before use, vortex thoroughly or invert to mix, ensuring magnetic beads are uniformly dispersed before taking aliquots. Anti-aggregation performance – the hydrophilic surface modification and suspension stabilizer formulation used in this product effectively prevent irreversible aggregation of beads after long-term static storage. If slight sedimentation is observed, mixing restores uniform suspension. Usage precautions – do not freeze the magnetic bead suspension; freezing destroys suspension stability and causes bead aggregation. Mix thoroughly before each use and cap tightly immediately after use to avoid dust and RNase contamination.

YALEPIC Tryzol Plus features two key optimizations over the traditional Trizol method: First, it uses TryCOM as a chloroform substitute for phase separation, dramatically reducing toxic reagent exposure risk and making operation safer and more environmentally friendly. Second, the kit includes a dedicated RNA dissolution buffer, ensuring that extracted RNA dissolves fully and has better stability. The overall lysis principle and workflow remain consistent with the classic Trizol method, enabling seamless switching from existing laboratory Trizol extraction protocols without the need to re-optimize experimental conditions.

This kit is primarily designed for total RNA extraction. During the phase separation step, RNA partitions into the upper aqueous phase, while DNA and protein reside in the interphase and lower organic phase, respectively. If needed, users can separately collect the interphase and lower organic phase and recover genomic DNA and protein through isopropanol/ethanol precipitation methods, enabling simultaneous recovery of RNA, DNA, and protein from a single sample, ideal for comprehensive utilization of precious samples.

Total RNA extracted with the YALEPIC Tryzol Plus kit shows high purity, with A260/A280 ratios typically stable between 1.9 and 2.1, with almost no protein or genomic DNA contamination. It can be directly used for downstream experiments with high RNA purity requirements such as RT-PCR, Real-Time PCR, Northern Blot, and RNA-Seq without additional purification steps.

TryCOM has far lower toxicity and volatility than traditional chloroform, significantly improving usage safety. Routine operations can be performed on a standard lab bench without mandatory fume hood use. However, since Tryzol Reagent itself contains guanidine isothiocyanate and phenol components, it is still recommended to wear disposable gloves, a mask, and goggles throughout the operation and maintain basic laboratory ventilation.

The YALEPIC Tryzol Plus RNA Extraction Kit has a very broad application range and has been successfully applied to total RNA extraction from various sample types including animal tissues (liver, brain, muscle, etc.), cultured cells (adherent/suspension cells), plant tissues (leaves and other materials with low secondary metabolites), microorganisms (bacteria, yeast), viral samples, and body fluids (blood, serum). For plant samples with high polysaccharide/polyphenol content (e.g., mature fruits, bark), YALI BIOTECH’s plant-specific RNA extraction kit CAT#YR23016 is recommended for better results.

Completing total RNA extraction for a batch of samples with this kit takes approximately 30-60 minutes (depending on sample number). The main steps are: sample lysis -> add TryCOM for phase separation -> centrifuge and collect aqueous phase -> isopropanol precipitation of RNA -> ethanol wash -> dissolve RNA. The workflow is essentially the same as the classic Trizol method, requires no special equipment other than a standard benchtop centrifuge, has a low learning curve, and is suitable for both novice and experienced researchers.

This kit (CAT#YR23014) does not include DNase I because the optimized phase separation process yields total RNA with extremely low genomic DNA carryover, which is directly usable for most routine RT-PCR and qPCR experiments. If your downstream experiments have stringent DNA carryover requirements (e.g., RNA-Seq library construction, low-abundance gene detection), it is recommended to additionally use YALI BIOTECH’s DNase I products (e.g., gDNA removal kit) for DNA digestion to ensure experimental accuracy.

Dissolve the extracted total RNA in the kit’s RNA dissolution buffer or Nuclease-free Water, aliquot, and store at -80 degrees C, which can preserve it effectively for 6-12 months. For short-term use (within 1-2 weeks), -20 degrees C storage is acceptable. Avoid repeated freeze-thaw; aliquot as needed. Keep dissolved RNA on ice during operation to avoid RNase degradation.

YALEPIC Tryzol Plus is a domestically developed RNA extraction kit by YALI BIOTECH. While maintaining extraction performance and quality comparable to imported equivalent products, its price is only 1/3 to 1/2 of imported products. The 100 mL specification catalog price is only 350 CNY, sufficient for approximately 100 routine sample extractions (approximately 1 mL per use), with a per-extraction cost as low as approximately 3.5 CNY, making it highly suitable for large-volume routine use in research laboratories, effectively reducing consumable costs.

You can inquire online through the YALI BIOTECH official website, or contact our sales team by calling the service hotline at 0512-66256010 or emailing info@yalibiotech.com. This kit supports bulk purchasing with exclusive volume discounts. YALI BIOTECH also provides OEM/ODM customization services to prepare different packaging specifications or special formulations according to customer needs. Research institutes, biotech companies, and distributors are welcome to inquire about cooperation.

This is a standard 50T kit (catalog YR23017), providing 50 RNA extraction reactions. It is a universal tissue/cell RNA extraction kit suitable for both animal tissues (liver, spleen, kidney, brain, etc.; recommended 10-30 mg) and cultured cells (adherent/suspension; recommended up to 1×10^7 cells), eliminating the need for separate kits.

Suitable for various common animal soft tissues including liver, spleen, kidney, brain, heart, skeletal muscle, and lung. Note: DNA-rich tissues (liver, spleen, kidney) should use no more than 10-20 mg to avoid affecting extraction and increasing gDNA residue risk.

The entire rapid RNA extraction process can be completed in 7 minutes at room temperature – from sample lysis to RNA elution, with no pre-cooled centrifuge or ice-bucket operation needed. All centrifugation is performed at room temperature (15-30 degrees C) using a standard benchtop centrifuge (>=12,000 rpm). This 7-minute room-temperature extraction is a core advantage, compared to traditional TRIzol (~1.5 hours) and conventional column methods (~30 minutes).

Completely unnecessary. This product uses silica membrane column purification technology, with no chloroform, phenol, or beta-mercaptoethanol needed. This is very friendly for laboratory safety management and operator health, especially suitable for labs sensitive to organic reagents or with limited ventilation.

Yes. The kit uses a dual-column filtration system – Fast gDNA Filter Columns effectively remove genomic DNA, combined with optimized wash buffers to remove protein and salt residues. Total RNA purity A260/A280 is typically 1.9-2.1, directly usable for RT-PCR, qPCR, Northern Blot, and other high-purity-requirement downstream experiments. For complete DNA removal (especially for low-abundance gene expression analysis), use RNase-free DNase I for on-column or post-elution digestion.

Yes. The kit includes Fast gDNA Filter Columns for one-step efficient gDNA removal during extraction, with no additional steps needed. For DNA-rich samples (liver, spleen, kidney), keep starting amount to 10-20 mg. For highly DNA-sensitive downstream experiments (qPCR), use RNase-free DNase I for further digestion after RNA elution. Detailed instructions are in the protocol.

All components can be stored dry at 10-30 degrees C room temperature, no refrigeration or freezing needed. Transport at room temperature without cold chain support. This provides great convenience for laboratory inventory management and reduces transport/storage costs. After opening, store at room temperature until the kit expiry date (see bottle label), but avoid repeated opening that may introduce contamination.

All components are designed for stability throughout the shelf life (typically 12-24 months, see bottle label). Tighten caps after each use and store in a clean, dry room temperature environment. Avoid prolonged exposure of lysis and wash buffers to air to prevent solvent evaporation or RNase contamination. No freeze-thaw needed (all components stored at room temperature).

Column clogging is typically caused by: (1) Excessive sample input – refer to recommended amounts (tissue 10-30 mg, cells up to 1×10^7); (2) Insufficient grinding/lysis – grind thoroughly, increase lysis buffer volume and extend lysis time if needed. Do not aspirate precipitate when transferring supernatant to the column. See the troubleshooting section in the protocol for a complete guide.

Low RNA yield may be caused by: insufficient sample (increase amount, but tissue <30 mg, cells <1×10^7); sample degradation from improper storage (use fresh or liquid nitrogen flash-frozen samples at -85 to -65 degrees C, avoid freeze-thaw); insufficient grinding/homogenization (increase lysis buffer volume and time); insufficient elution (add RNase-free ddH2O to membrane center, reduce elution volume, pre-warm to 56 degrees C or extend room temperature incubation, or perform double elution).

The high-purity, high-integrity total RNA is suitable for RT-PCR, real-time quantitative PCR (qPCR/Real-Time RT-PCR), microarray analysis, Northern Blot, Dot Blot, Poly(A) RNA selection, in vitro translation, RNase protection assay, molecular cloning, and more. Products can be used directly or stored long-term at -85 to -65 degrees C.

A1: The YALEPIC Plant Total RNA Rapid Isolation Kit (PLUS) is a universal product suitable for both common plant leaves (e.g., rice, maize, Arabidopsis, soybean, etc.) and specifically optimized for complex samples rich in polysaccharides and polyphenols, including tubers (potato), root tubers (sweet potato), seeds, fruit flesh (apple, grape, etc.), and fungal mycelia. One kit covers both common and complex samples — no need to purchase separate product lines.

A2: This kit uses a column-based rapid extraction protocol with the entire process performed at room temperature. From sample grinding and lysis to RNA elution takes only about 10 minutes, making it one of the fastest plant RNA extraction kits on the market. The workflow is straightforward — no phenol-chloroform extraction or low-temperature centrifugation required. Even beginners can quickly master the procedure.

A3: This kit employs a unique dual-column filtration system (Fast gDNA Filter Columns + Pure Columns RT) that efficiently removes genomic DNA during the extraction process. For most samples, no additional DNase I digestion is needed. For experiments with extremely stringent gDNA residue requirements (e.g., low-abundance gene RT-qPCR), a post-extraction DNase I treatment step can be added to ensure complete removal.

A4: No. This kit uses a proprietary safe lysis buffer formulation — no phenol-chloroform, beta-mercaptoethanol, or other traditional toxic reagents are needed throughout the entire extraction. This makes it safer and more environmentally friendly, particularly suitable for teaching laboratories, clinical testing facilities, and labs without hazardous chemical handling capabilities.

A5: The extracted total RNA has been verified by 1.2% agarose gel electrophoresis — 28S and 18S rRNA bands are clear with normal ratios, indicating good integrity. NanoDrop measurements typically show A260/A280 ratios between 1.9 and 2.1, indicating high purity. The RNA can be directly used for RT-PCR, real-time quantitative PCR (RT-qPCR), Northern Blot, microarray analysis, Poly(A) selection, in vitro translation, RNase protection assays, molecular cloning, and other downstream experiments.

A6: Common problems with polysaccharide- and polyphenol-rich plant samples (e.g., grape, apple flesh, potato tuber) include: polysaccharide co-precipitation making RNA difficult to dissolve, polyphenol oxidation causing RNA browning and degradation, and severe gDNA residue. This kit addresses these through an optimized lysis buffer that effectively inhibits polyphenol oxidation, a silica membrane that specifically adsorbs RNA combined with efficient wash buffers to remove polysaccharide residue, and a dual-column system that simultaneously eliminates gDNA contamination — significantly improving RNA extraction success rates for complex samples.

A7: Yes. This kit has been validated with various fungal mycelium samples and is suitable for total RNA extraction from filamentous fungi and other fungal samples. The recommended input for fungal mycelia is 20-100 mg. After thorough grinding, follow the standard protocol to obtain high-quality RNA.

A8: Common causes and solutions for low RNA yield: (1) Insufficient sample input — use the recommended amounts in the protocol; (2) Incomplete sample grinding — grind to a fine powder in liquid nitrogen and quickly transfer to lysis buffer; (3) Improper sample storage — use fresh samples or samples stored at -80°C without repeated freeze-thaw cycles; (4) Incomplete elution — apply elution buffer (RNase-free ddH2O) to the center of the membrane, optionally pre-warm to 56°C, extend room temperature incubation time, or perform a second elution to improve recovery.

A9: Column clogging is typically caused by: (1) Excessive sample input — reduce the starting material amount; (2) Incomplete grinding leaving large particles in the lysate — grind thoroughly, optionally increase lysis buffer volume and extend centrifugation time, and avoid pipetting precipitate when transferring supernatant. If the problem persists, contact YALI Biotech’s technical support team for further assistance.

A10: The YALEPIC Plant Total RNA Rapid Isolation Kit (PLUS) has catalog number YR23016, specification 50 T/kit, list price ¥850.00. Bulk purchase discounts are available. For inquiries or orders, call YALI Biotech’s service hotline at +86-512-66256010, email info@yalibiotech.com, or submit a request through the online contact form on our website.

Yes. The kit includes DNase I and its matching buffer for in-situ enzymatic removal of genomic DNA during the purification workflow, without additional treatment, effectively preventing DNA interference in downstream experiments.

No. This kit uses a TryCOM proprietary component instead of chloroform, containing no toxic organic solvents. Operation is safer and suitable for routine laboratory and teaching environments.

It is suitable for a wide range of sample types including animal tissues, cultured cells, microorganisms, plant materials with low secondary metabolites, viruses, and body fluids, offering strong versatility.

It can be directly used for RT-PCR, qPCR, gene chips (microarray), Northern Blot, Poly(A) selection, in vitro translation, molecular cloning, and other downstream molecular biology experiments.

DNase I and 10x DNase Buffer should be stored at -20 degrees C; Tryzol Reagent at 2-8 degrees C protected from light; all other components at room temperature. Strict adherence to storage conditions ensures stable shelf life.

This kit is better suited for plant materials with low secondary metabolite content. For complex plant samples rich in polysaccharides and polyphenols, YALI BIOTECH’s dedicated plant RNA extraction kit is recommended.

The spin column method is simpler and faster to operate with better reproducibility, more stable RNA purity and yield, and eliminates the need for isopropanol precipitation, reducing loss and contamination risk.

Routine animal tissue: approximately 50-100 mg; cultured cells: approximately 10^6-10^7 cells; plant tissue: <=100 mg. Refer to the product manual for specific optimization.

The YALEPIC Blood RNA Extraction Kit is suitable for fresh whole blood from humans, mice, rats, rabbits, pigs, cattle, sheep, and other mammals. Compatible with EDTA, citrate, heparin, and other common anticoagulants, covering clinical testing, basic research, and veterinary needs.

The recommended single processing volume is up to 1.5 mL of whole blood (for larger volumes, consult YALI Biotech technical team). Within this range, stable RNA yield and purity are achieved. For larger volumes, split and process in batches to ensure thorough lysis and stable RNA quality.

NanoDrop testing shows A260/A280 typically >=1.9 and A260/A230 >=1.5, indicating high purity with effective removal of heme, heparin, and other PCR inhibitors. The extracted RNA can be directly used for RT-PCR, Real-Time RT-qPCR, and other experiments without additional purification.

This product (catalog YR23003) does not contain DNase I. For downstream experiments sensitive to genomic DNA residue (e.g., RT-qPCR quantitative analysis), we recommend using YALI Biotech DNase I (sold separately) for on-column or off-column DNase digestion after RNA extraction to thoroughly remove genomic DNA contamination.

No. The YALEPIC Blood RNA Extraction Kit uses column-based purification technology, with no phenol, chloroform, or other toxic organic reagents needed. The operation is safe and environmentally friendly, compliant with modern green laboratory standards, especially suitable for researchers who perform frequent extractions.

Yes. The extracted blood total RNA has good integrity (clear 28S/18S rRNA bands on agarose gel) and high purity, fully meeting the requirements of RNA-Seq high-throughput sequencing, microarray analysis, and other high-quality-RNA downstream experiments.

All components can be stored at 10-30 degrees C room temperature, no refrigeration or freezing needed. Room temperature transport without dry ice or ice packs effectively reduces logistics and storage costs. Store sealed and protected from light; use promptly after opening.

For best RNA quality, extract within 2 hours of blood collection. If immediate processing is not possible, anticoagulated whole blood can be temporarily stored at 2-8 degrees C (no more than 24 hours), or white blood cells can be separated and stored at -80 degrees C. For long-term storage, use YALI Biotech blood RNA sample storage tubes for room temperature stabilization.

The blood RNA extraction kit is specifically optimized for whole blood, with a lysis system that thoroughly lyses blood cells and effectively removes heme and other blood-specific inhibitors. The tissue RNA extraction kit is designed for solid tissue mechanical homogenization and protein removal. Choose the appropriate kit based on sample type for best results.

Yes. YALI Biotech supports bulk procurement and OEM/ODM customization, including different specifications, custom labels, and technical support. For large-volume or customization needs, contact us via the website or call 0512-66256010 for a dedicated quote.

The biggest difference lies in safety and operational convenience. The traditional Trizol method requires the use of highly toxic chloroform for extraction and isopropanol precipitation, which is time-consuming and poses health risks to operators. YALEPIC Tryzol Plus is equipped with an environmentally friendly TryCOM chloroform substitute, achieving chloroform-free RNA extraction that is safe and non-toxic. At the same time, combined with silica-membrane spin column technology, it eliminates the tedious precipitation step, shortens extraction time to about 30 minutes, and yields RNA of even higher purity.

This kit version is the ‘DNase I-Free’ version. If your downstream experiments (e.g., high-precision RT-qPCR) are extremely sensitive to genomic DNA carryover, it is recommended to perform on-column or in-solution digestion using YALI BIOTECH’s YALEPIC DNase I (RNase-free) CAT#YX27002 during extraction. Alternatively, purchase the YALEPIC Tryzol Plus spin column RNA extraction kit with DNase I (CAT#YR23009-C) directly.

Yes. For plant tissues with low secondary metabolite content (e.g., low polysaccharide/polyphenol), this kit performs well. For plants rich in polysaccharides and polyphenols, YALI BIOTECH’s plant-specific RNA extraction kit is recommended. For blood samples, it is recommended to first remove red blood cells with red blood cell lysis buffer, collect white blood cells, and then use this kit for extraction to achieve higher RNA yield and purity.

Low A260/A280 is usually due to protein contamination. It is recommended to increase the number of wash buffer steps or ensure complete lysis. If A260/A280 is abnormally high (>2.2), it may indicate partial RNA degradation or impurities in the elution buffer. Ensure that all labware is strictly RNase-free, perform operations on ice, and use the kit’s dedicated RNase-free Water for elution.

Yes, it can be used normally. During storage at 2-8 degrees C, some salt components in the lysis buffer may crystallize out. Before use, remove the reagent and let it stand at room temperature (15-25 degrees C) or in a 37 degrees C water bath to dissolve completely. After thorough shaking, it will regain its original performance without affecting RNA extraction results.

This kit is suitable for extracting viral, bacterial, and fungal DNA and RNA from blood (fresh or frozen anticoagulated whole blood, <=400 microL), body fluids (bronchoalveolar lavage fluid, cerebrospinal fluid, pleural or ascitic fluid, sputum, etc., 2-3 ml), swab fluid (nasal swabs, throat swabs, oral swabs, etc., 2-2 ml), bacterial and fungal cultures (<=1×10^9 cells), and other biological samples.

The extracted nucleic acids have high purity and good integrity, and can be directly used for RT-PCR, real-time quantitative PCR (qPCR), Northern blot, SNP genotyping, STR genotyping, in vitro translation, cDNA library construction, pharmacogenomics research, metagenomic sequencing, and other molecular biology experiments.

This kit uses silica gel spin column adsorption technology. Under a unique buffer system, microbial nucleic acids in the lysis buffer efficiently and specifically bind to the silica membrane. After centrifugation washing to remove proteins, cellular metabolites, and other impurities, high-purity nucleic acids are eluted from the column using a low-salt elution buffer. This technology is convenient to operate and does not require toxic reagents such as phenol-chloroform.

Yes. This kit is specifically designed for simultaneous extraction of pathogenic microbial DNA and RNA from samples, eliminating the need for separate extraction kits, effectively saving reagent costs and operation time.

Yes. This kit uses an optimized lysis buffer system that can efficiently lyse Gram-positive bacteria, Gram-negative bacteria, and fungi with thick cell walls, improving the detection rate of low-abundance pathogenic microorganisms.

GBA Buffer should be stored frozen at -20 degrees C; avoid repeated freeze-thaw. Other components can be stored at room temperature (10-30 degrees C). Under the recommended storage conditions, the shelf life of all components is 18 months. After use, tighten caps promptly to prevent evaporation or pH changes.

GBA Buffer requires low-temperature transport (ice packs or dry ice recommended); other components can be transported at room temperature.

Freshly collected samples that can be delivered to the laboratory within 48 hours can be stored and transported at 4 degrees C. For long-term storage or extended transport time, freeze samples (-20 degrees C or -80 degrees C). Swab samples should be stored and transported in preservation medium. To ensure downstream RNA extraction quality, avoid repeated sample freeze-thaw.

Possible causes include: (1) Insufficient starting sample amount or improper sample storage leading to nucleic acid degradation; (2) Insufficient lysis, especially for bacteria or fungi with thick cell walls; (3) Improper washing or elution operations. Follow the instruction manual carefully and ensure samples are fresh or stored under appropriate conditions.

This kit currently uses the spin column method and is designed for manual extraction. For automated extraction solutions, refer to YALI Biotech’s YALEPIC MagEVO series magnetic bead-based pathogenic microbe DNA/RNA extraction kits, which are compatible with both manual extraction and automated extraction programs.

The kit components are RNase-free processed to ensure that RNA extraction is not affected by RNase degradation, guaranteeing RNA integrity and purity.

YALEPIC Pathogenic Microbes DNA/RNA Extraction Kit, catalog number YB25004, specification 50T, list price 1250.00 CNY. For bulk purchases or other specification needs, contact the YALI Biotech sales team for customized quotes.

The spin column kit (this product, catalog number YB25004) uses silica column centrifugation technology, suitable for manual operation and low-throughput sample processing, requiring no special equipment. The magnetic bead kit (YALEPIC MagEVO series) uses magnetic bead purification technology, compatible with automated nucleic acid extraction instruments, suitable for high-throughput sample processing and automated laboratories. Users can select the appropriate product based on sample throughput and laboratory equipment conditions.

Yes. This kit uses a co-extraction buffer formulation, enabling simultaneous recovery of both viral DNA and RNA from a single sample in one extraction without performing two separate lysis steps, saving sample material and experimental time. It is the preferred extraction reagent for respiratory virus and mixed pathogen samples.

Model A (32T) is low-throughput, suitable for small labs processing occasional swab samples. Model B (48T) is designed for small-to-medium routine testing. Model C (96T) is a pre-filled plate format compatible with automated nucleic acid extractors for high-throughput batch runs, eliminating the need for manual reagent dispensing.

The product formulation has been validated for high-temperature stability. It can be stored at 10-30 degrees C in the dark at room temperature. In summer, as long as the ambient temperature does not exceed 30 degrees C, refrigeration is not needed. Simply avoid direct sunlight to maintain normal shelf life. Room-temperature shipping will not affect reagent activity.

Yes. The optimized lysis buffer can break through mucus and fecal impurities. For thick sputum, pre-dilute with saline. For fecal samples, use the supernatant for extraction to significantly improve nucleic acid yield and purity. Alternatively, YALI BIOTECH offers dedicated nucleic acid extraction kits for saliva and feces samples.

Yes. The purified nucleic acids are free of protein and salt inhibitors and can be directly used for qPCR, RT-PCR, and whole-genome next-generation sequencing without secondary purification, meeting both research and clinical testing standards.

It is universally compatible. The standard 96T version supports manual reagent dispensing and manual extraction. The pre-filled A/B/C models are optimized for the YALEPIC Pure 32 automated magnetic bead extraction system and are also compatible with most mainstream domestic and imported nucleic acid extractors.

For simple swab and plasma samples, the complete lysis-binding-washing-elution workflow can be finished in as little as 30 minutes.

The recommended sample volume is generally 100-200 microL. Plasma and serum can be loaded directly. For whole blood samples, it is recommended to first separate the plasma before extraction to reduce heme impurity interference.

Yes. All three pre-filled models come with pre-dispensed magnetic beads, lysis buffer, wash buffer, and elution buffer, eliminating the need for manual reagent aliquoting, reducing pipetting errors, and shortening pre-processing time.

Common causes include repeated freeze-thaw of samples, low viral load in the sample, and insufficient lysis mixing. Using fresh samples and thorough vortex lysis can effectively improve nucleic acid recovery.

It is fully compatible. The kit has been extensively validated for DNA/RNA extraction from livestock and poultry serum, throat swabs, and tissue samples for animal viruses, and is widely used for farm disease screening.

After opening, keep the original packaging sealed and store at 10-30 degrees C protected from light. Seal the dispensed magnetic bead solution tightly and avoid environmental RNase contamination. Use within 1 month for optimal extraction efficiency.

This kit is suitable for various clinical and environmental samples, including: fresh or frozen anticoagulated whole blood (<=400 microL), nasopharyngeal swabs, sputum, bronchoalveolar lavage fluid, cerebrospinal fluid, pleural or ascitic fluid and other body fluid samples (2-3 ml, <=5×10^6 cells), bacterial (<=1×10^9 cells) and fungal (<=1×10^9 cells) microbial cultures, and also supports DNA/RNA extraction from stool samples (100 mg solid or 200 microL stool in preservation medium).

Yes. The kit is specifically optimized for co-extraction of pathogenic microbial DNA and RNA, capable of simultaneously extracting and purifying DNA and RNA from viruses, bacteria, and fungi in the same sample. This feature is particularly suitable for metagenomic sequencing and pathogen co-detection applications requiring simultaneous DNA virus and RNA virus detection.

This kit uses a lysis system combining chemical lysis and mechanical grinding, with lysis optimization for fungi (such as yeast, Candida) and Gram-positive bacteria with thick cell walls that are difficult to lyse. It can efficiently release nucleic acids, significantly improving the detection rate of such microorganisms, suitable for low-abundance pathogen detection in clinical samples.

The purified DNA/RNA products have high purity, good integrity, and no protein or nuclease residue, suitable for various downstream molecular biology applications, including: conventional PCR and real-time quantitative PCR (qPCR), metagenomic library construction (mNGS), whole-genome sequencing (WGS), 16S rDNA-based microbiome analysis, and shotgun metagenomic sequencing analysis.

Yes. This kit uses magnetic bead-based technology, compatible with both manual extraction using a magnetic rack and fully compatible with various magnetic rod-based and pipetting-based automated nucleic acid extraction instruments for high-throughput extraction, suitable for large-scale sample processing needs in clinical testing centers, CDC, and research laboratories.

No. This kit uses nano-magnetic bead purification technology, requiring no phenol, chloroform, or other toxic organic solvents throughout the process. The extraction process does not involve organic reagent extraction steps, making it safer for laboratory personnel and compliant with green environmental laboratory practices.

This kit supports ambient temperature transport, requiring no cold chain throughout. All components can be stored at room temperature (10-30 degrees C), with a shelf life of 12 months. These storage conditions greatly reduce cold chain costs during transport and storage, facilitating laboratory inventory and daily use.

The MagEVO kit is optimized for high yield and good reproducibility. The purified DNA/RNA products typically have an A260/A280 ratio between 1.8-2.0, with low protein residue. The purified products are free of PCR inhibitors and nucleases, and can be directly used for downstream amplification and sequencing reactions without additional purification.

Yes. This kit has undergone specific buffer optimization for sputum, bronchoalveolar lavage fluid, and other complex body fluid samples rich in mucin and various PCR inhibitors. Combined with efficient washing steps, it can effectively remove inhibitors from samples, ensuring that the extracted nucleic acid purity meets downstream detection requirements.

mNGS has high requirements for nucleic acid extraction purity, integrity, and background bacterial contamination control. This kit uses an efficient washing system that maximally removes host nucleic acid interference and background bacterial contamination, with purified products suitable for mNGS library construction. Use NGS-grade water and nuclease-free consumables during mNGS experiments, and set up negative controls during extraction to monitor background contamination.

The YALEPIC Viral DNA/RNA Extraction Kit is suitable for various sample types including whole blood, serum, plasma, sputum, lymph fluid, tissue homogenate, cell culture supernatant, urine, and various viral transport media (VTM). It is also suitable for nasopharyngeal swabs, oropharyngeal swabs, bronchoalveolar lavage fluid (BALF), and other respiratory samples for viral nucleic acid extraction.

Manual extraction for a single sample takes approximately 15 minutes. This kit uses the spin column method and supports simultaneous processing of multiple samples, limited only by centrifuge capacity. For high-throughput processing, YALI Biotech also offers a magnetic bead-based viral DNA/RNA extraction kit compatible with automated extraction platforms.

This kit does not contain phenol, chloroform, or other toxic organic solvents. The entire process is safe and convenient without exposure to toxic chemicals. Extraction can be performed on a standard lab bench without a fume hood (though processing potentially infectious samples should still be done in a biosafety cabinet). Waste disposal follows standard laboratory protocols, greatly reducing health risks.

The extracted viral DNA and RNA have high purity with thorough removal of inhibitors. They can be directly used for RT-PCR, real-time quantitative PCR (qPCR), Northern Blot, SNP genotyping, STR genotyping, in vitro translation, cDNA library construction, pharmacogenomics research, metagenomic sequencing, and more. We recommend evaluating extract compatibility based on specific downstream requirements.

The silica membrane adsorption technology maintains good binding efficiency even for low-concentration nucleic acids. For extremely low viral load samples, appropriately increase the starting sample volume (within the recommended range) or use high-sensitivity qPCR for detection. Note that for extremely low copy number samples, conventional OD measurement or electrophoresis may not detect the nucleic acids; qPCR or similar high-sensitivity methods are recommended.

Extracted viral DNA/RNA should be eluted with the kit’s elution buffer or RNase-free water. Short-term storage (within 24 hours) at 2-8 degrees C is acceptable. For long-term storage, aliquot and store at -20 degrees C (up to 6 months) or -80 degrees C (up to 12 months), avoiding repeated freeze-thaw. RNA samples should be protected from RNase contamination using RNase-free consumables and reagents.

Possible causes: (1) Low viral load in sample – increase starting volume; (2) Insufficient elution – pre-warm elution buffer to 65 degrees C before adding to the column; (3) Ethanol residue from wash steps – residual ethanol inhibits enzymatic reactions, spin the empty column for 2 minutes to remove ethanol completely; (4) Downstream assay issues – set up positive controls for troubleshooting. If problems persist, contact YALI Biotech technical support.

Core advantages: (1) Speed – only 15 minutes per sample, significantly faster than traditional column-based products; (2) Safety – completely free of toxic organic reagents; (3) Cost-effectiveness – competitive pricing while maintaining high purity and yield; (4) Flexibility – supports 200-400 microL variable sample volumes and multiple sample types; (5) Support – professional technical support team providing full-process service from experimental design to troubleshooting.