YALEPIC® Endotoxin Free Plasmid DNA Mini Isolation Kit (1-5ml)

Standard adaptation is for 1-5 mL of overnight culture. For low-copy plasmids, the culture volume can be increased to within 10 mL by proportionally scaling up the volumes of Buffer PA, PB, and ER for normal extraction.

The entire process takes only 35 minutes to complete plasmid extraction, using a modified alkaline lysis method combined with silica membrane binding technology. No toxic reagents such as phenol or chloroform are required. The steps are simplified and even novices can quickly get started.

The endotoxin carryover of the product is <1 EU/microgram, meeting professional transfection-grade standards, with no toxicity to eukaryotic cells, and can be directly used for various sensitive cell transfection experiments.

The maximum plasmid DNA yield per sample can reach up to 45 microgram, with high purity and no RNA or protein carryover, fully meeting the requirements of downstream experiments such as gene sequencing, restriction enzyme digestion, PCR, and in vitro transcription.

It supports ambient-temperature shipping. For daily storage, it only needs to be kept sealed at 10-30 degrees C room temperature; -20 degrees C cold storage is not required, making storage and transport more convenient.

A pre-mixed proprietary color indicator allows intuitive judgment of whether cell lysis and neutralization reactions are complete through liquid color change, achieving visualized operation and effectively avoiding extraction failure due to improper experimental handling.

The 50T specification can complete 50 standard 1-5 mL culture plasmid extraction experiments, with ample reagent ratios and high cost-effectiveness, suitable for routine batch experiments in laboratories.

Endotoxin-free plasmid purified with this kit has high purity and no impurity contamination, can be routinely aliquoted and stored long-term at -20 degrees C, resists degradation, and maintains good reproducibility of experiments.