YALEPIC^® Stool RNA Sample Storage Tube

Liquid nitrogen snap-freezing can instantly stop enzyme activity, but transport requires dry ice throughout, which is costly and not user-friendly for at-home sampling. This preservation fluid achieves a chemical snap-freeze effect at room temperature, stable for 14 days at room temperature, then can be directly couriered to a central laboratory and transferred to -80 degrees C. Most metatranscriptomic data show no significant difference in downstream RNA-seq gene expression profiles between the two methods, but the advantage in logistics convenience is significant.

Loose stool has high water content; it is recommended to appropriately increase the ratio of preservation fluid to stool, such as 1:4 or 1:5 (w/v), and vortex thoroughly. If the sample amount is small, it can also be collected directly into a collection tube pre-loaded with preservation fluid, where the liquid protectant quickly encapsulates and protects free RNA. Validation data show that watery stool can also be stably preserved at room temperature for 14 days.

We compared fresh frozen samples with preservation fluid samples stored at room temperature for 7 days using metatranscriptomic sequencing. PCoA and differential gene expression analysis both showed high similarity between the two groups (correlation >0.95), indicating that this preservation fluid effectively inhibits in vitro transcription of bacterial communities, avoiding ex vivo stress expression shifts.

Compatible. It is recommended to centrifuge the stool-preservation fluid suspension, discard the supernatant, and then lyse with TRIzol reagent. If TRIzol is added directly, the high content of preservation fluid components may slightly affect phase separation efficiency; an additional PBS wash can eliminate this effect. Using a silica column or magnetic bead-based stool RNA extraction kit is more recommended, as it yields higher purity and quantity of RNA.

Conventional electrophoresis shows clear 18S and 28S rRNA bands (eukaryotic samples) or 23S/16S bands (prokaryotic samples), with RIN values generally no less than 7. We have performed stool metatranscriptomic library construction and sequencing in multiple client projects. After rRNA removal, the proportion of effective reads is excellent, and gene coverage and expression correlation meet the requirements for high-impact publication.

The preservation fluid can rapidly inactivate most enveloped viruses and some non-enveloped viruses, significantly reducing biosafety risks. Samples comply with UN3373 Category B biological substance transport requirements. However, sealed leak-proof collection tubes must be used, outer packaging must be labeled according to biosafety regulations, and there are no special requirements for room temperature courier shipping.

Not recommended. Repeated opening and freeze-thaw will introduce moisture, oxidation, and microbial contamination, while physical disturbance may activate residual nucleases. It is recommended to aliquot into single-use tubes after the first mixing, store sealed at -80 degrees C, and use as needed.

Ready-to-use collection tubes in 5 ml and 30 ml sizes are available, pre-filled with sterilized and filtered preservation fluid, with an attached sampling spoon. Suitable for different volumes of stool samples, with custom labeling and packaging supported. Contact sales or authorized distributors for details.

Some products on the market are high-viscosity gels that do not easily infiltrate loose stool; others require -20 degrees C storage and transport. YALI Biotech’s product is a low-viscosity liquid that quickly coats stool of all consistencies, truly enabling room temperature transport, with competitive pricing. Comparative experiments show lower transcriptional induction effects on the metatranscriptome, with excellent RNA yield and integrity.