YALEPIC^® Pathogenic Microbes DNA/RNA Extraction Kit

This kit is suitable for extracting viral, bacterial, and fungal DNA and RNA from blood (fresh or frozen anticoagulated whole blood, <=400 microL), body fluids (bronchoalveolar lavage fluid, cerebrospinal fluid, pleural or ascitic fluid, sputum, etc., 2-3 ml), swab fluid (nasal swabs, throat swabs, oral swabs, etc., 2-2 ml), bacterial and fungal cultures (<=1x10^9 cells), and other biological samples.

The extracted nucleic acids have high purity and good integrity, and can be directly used for RT-PCR, real-time quantitative PCR (qPCR), Northern blot, SNP genotyping, STR genotyping, in vitro translation, cDNA library construction, pharmacogenomics research, metagenomic sequencing, and other molecular biology experiments.

This kit uses silica gel spin column adsorption technology. Under a unique buffer system, microbial nucleic acids in the lysis buffer efficiently and specifically bind to the silica membrane. After centrifugation washing to remove proteins, cellular metabolites, and other impurities, high-purity nucleic acids are eluted from the column using a low-salt elution buffer. This technology is convenient to operate and does not require toxic reagents such as phenol-chloroform.

Yes. This kit is specifically designed for simultaneous extraction of pathogenic microbial DNA and RNA from samples, eliminating the need for separate extraction kits, effectively saving reagent costs and operation time.

Yes. This kit uses an optimized lysis buffer system that can efficiently lyse Gram-positive bacteria, Gram-negative bacteria, and fungi with thick cell walls, improving the detection rate of low-abundance pathogenic microorganisms.

GBA Buffer should be stored frozen at -20 degrees C; avoid repeated freeze-thaw. Other components can be stored at room temperature (10-30 degrees C). Under the recommended storage conditions, the shelf life of all components is 18 months. After use, tighten caps promptly to prevent evaporation or pH changes.

GBA Buffer requires low-temperature transport (ice packs or dry ice recommended); other components can be transported at room temperature.

Freshly collected samples that can be delivered to the laboratory within 48 hours can be stored and transported at 4 degrees C. For long-term storage or extended transport time, freeze samples (-20 degrees C or -80 degrees C). Swab samples should be stored and transported in preservation medium. To ensure downstream RNA extraction quality, avoid repeated sample freeze-thaw.

Possible causes include: (1) Insufficient starting sample amount or improper sample storage leading to nucleic acid degradation; (2) Insufficient lysis, especially for bacteria or fungi with thick cell walls; (3) Improper washing or elution operations. Follow the instruction manual carefully and ensure samples are fresh or stored under appropriate conditions.

This kit currently uses the spin column method and is designed for manual extraction. For automated extraction solutions, refer to YALI Biotech’s YALEPIC MagEVO series magnetic bead-based pathogenic microbe DNA/RNA extraction kits, which are compatible with both manual extraction and automated extraction programs.

The kit components are RNase-free processed to ensure that RNA extraction is not affected by RNase degradation, guaranteeing RNA integrity and purity.

YALEPIC Pathogenic Microbes DNA/RNA Extraction Kit, catalog number YB25004, specification 50T, list price 1250.00 CNY. For bulk purchases or other specification needs, contact the YALI Biotech sales team for customized quotes.

The spin column kit (this product, catalog number YB25004) uses silica column centrifugation technology, suitable for manual operation and low-throughput sample processing, requiring no special equipment. The magnetic bead kit (YALEPIC MagEVO series) uses magnetic bead purification technology, compatible with automated nucleic acid extraction instruments, suitable for high-throughput sample processing and automated laboratories. Users can select the appropriate product based on sample throughput and laboratory equipment conditions.