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YALEPIC® RNA High-Sensitivity Quantification Assay

High Recovery, Low Bias — Robust Assay for RNA-seq library quantification and RNA QC

A focused reagent for RNA-seq library quantification and RNA QC, engineered for high recovery and reproducibility.

Number Specifications Catalog Price Add To Cart
YNQ24004 1000rxns Online Consult +

Product Description

YALEPIC® RNA HS Assay Kit is a simple, sensitive and accurate RNA fluorescence quantitative detection kit, which contains fluorescence detection reagents, buffers and RNA standards. The kit is highly selective for RNA over double-stranded DNA (dsDNA). It has an excellent linearity for RNA samples in the range of 4 – 100 ng, allowing accurate quantification of total RNA, rRNA,  mRNA concentrations from 200 pg/μl to 200 ng/μl. In addition, it has good impurity tolerance to common contaminants, such as salts, free  nucleotides, proteins, solvents, detergents, etc. This product is easy to operate and can be carried out at room temperature. Before use, please dilute the fluorescence detection reagent into a working solution with buffer and add the RNA sample into it. The assay result will  be obtained by the Qubit Fluorometer.

Features

  • High specificity: This product is highly selective for RNA, not affected by DNA, and has good resistance to some conventional pollutants, such as salt, free nucleotides, proteins, solvents, detergents, etc. tolerance.
  • Easy to operate: This product is easy to operate. Dilute the fluorescence detection reagent with buffer solution into a working solution.

Application

It has an excellent linearity for RNA samples in the range of 4 – 100 ng, allowing accurate quantification of total RNA, rRNA,  mRNA concentrations from 200 pg/μl to 200 ng/μl.

Storage

Store at 2 ~ 8°C protect from light. Adjust the shipping method according to the estination.Do not freeze.

We suggest RNA HS Standard store at -20℃ for long-term storage.

What detection sensitivity can the YALEPIC RNA HS Assay Kit achieve? What low-concentration RNA samples is it suitable for?

This kit has a minimum detection limit of 1 pg/microL, with a linear range covering 1 pg/microL to 100 ng/microL, spanning 5 orders of magnitude. It is ideal for precise quantitative detection of ultra-low concentration RNA samples such as plasma cfRNA, serum cell-free RNA, urine RNA, exosome RNA, viral RNA (such as SARS-CoV-2, HBV, and other low-titer samples).

What is the difference between this RNA high-sensitivity assay kit and regular Qubit/NanoDrop? Why should NGS laboratories use it?

Qubit and NanoDrop are suitable for medium-to-high concentration RNA samples (typically >1 ng/microL), whereas liquid biopsy cfRNA, exosome RNA, and other samples often have concentrations below 1 ng/microL, which conventional methods cannot accurately detect. YALEPIC RNA HS uses a patented fluorescence-based quantification technology, specifically designed for pg-level ultra-low concentration RNA. It is a key quality control tool for ensuring accurate RNA input before NGS library construction, effectively preventing sequencing data bias caused by RNA quantification errors.

What instrument is needed for the YALEPIC RNA HS Assay Kit? Is the operation complex?

This kit is compatible with all mainstream real-time quantitative PCR instruments (such as ABI, Bio-Rad, Roche, Agilent, etc.), requiring no specialized equipment. The reagent is a pre-mixed formulation, ready to use out of the box with no additional preparation needed. From sample addition to results takes only about 30 minutes, with simple operation suitable for high-throughput laboratory batch testing.

How long does the 1000 rxns specification last? What is the cost per test?

The 1000 rxns specification is suitable for daily use in medium-to-large NGS laboratories. At one test per sample, 1000 rxns can test 1000 samples. The list price is 2925 CNY, making the cost per test only about 2.9 CNY. Bulk purchasing offers even more favorable pricing, providing excellent cost-effectiveness.

Can this kit be used for viral RNA load detection? For example, SARS-CoV-2 or HBV?

Absolutely. The ultra-high sensitivity (1 pg/microL) of this kit makes it ideal for precise quantification of viral RNA loads, including SARS-CoV-2, influenza virus, HBV, HCV, HIV, and more. Compared to traditional RT-qPCR quantification methods, this kit can first perform precise concentration measurement of extracted viral RNA, then guide the RNA input for subsequent qPCR reactions, significantly improving the accuracy and reproducibility of viral load detection.

What are the storage conditions for the kit? What are the transport requirements?

Please store at -20 degrees C protected from light, avoid repeated freeze-thaw cycles, and use within 6 months after opening. For transport, -20 degrees C dry ice shipping is recommended. If shipping at ambient temperature, use ice packs for insulation and deliver within 72 hours. Please refer to the accompanying COA report for specific details.

What are the advantages of YALI Biotech's YALEPIC RNA HS Assay Kit compared to other brands' RNA quantification kits?

Three core advantages: (1) Higher sensitivity – minimum 1 pg/microL, industry-leading; (2) Wider linear range – 5 orders of magnitude, covering trace to high concentrations in a single tube; (3) Better cost-effectiveness – 1000 rxns bulk packaging, with per-test cost as low as 2.9 CNY. Additionally, YALI Biotech provides comprehensive technical support and customized services, making it a premium choice for domestic high-throughput sequencing quality control reagents.

How to purchase YALEPIC RNA HS Assay Kit in bulk? Is there technical support?

For bulk purchases, please call 0512-66256010 to contact the YALI Biotech sales team directly. YALI Biotech provides professional pre-sales technical consultation and post-sales application support, and can offer customized solutions based on your experimental needs.

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