YALEPIC^® All-in RT Master Mix for qPCR (gDNA-Off)

This product is suitable for total RNA or mRNA templates from various sources including animal tissue, plant tissue, cultured cells, blood, viral samples, and more, covering mainstream application scenarios such as gene expression quantification and RNA virus detection.

The product pre-mixes a heat-labile dsDNase that specifically recognizes and degrades double-stranded DNA (dsDNA), including genomic DNA contamination, but has no effect on single-stranded DNA (ssDNA) or RNA. The heat-labile property allows this enzyme to rapidly inactivate during subsequent high-temperature reactions without affecting cDNA product integrity.

This product is a one-step All-in-Mix kit. Genomic DNA removal and cDNA synthesis are completed simultaneously in the same reaction tube, eliminating step-by-step operations, significantly shortening experiment time, reducing pipetting steps, and thereby lowering sample contamination risk and operational errors. Traditional two-step methods require gDNA removal first, then separate reverse transcription reactions, making the workflow more cumbersome.

The new-generation high-temperature reverse transcriptase YALEPIC Script Reverse Transcriptase Plus paired with this product has activity in the 50-60 degrees C range, with 55 degrees C as its optimal reaction temperature. High temperature conditions help unwind complex secondary structures of RNA templates, improving reverse transcription efficiency and cDNA length integrity.

Synthesized cDNA is recommended for short-term storage at -20 degrees C and long-term storage at -80 degrees C. Avoid repeated freeze-thaw to maintain the stability and reproducibility of qPCR quantification results.

No additional reverse transcription primers are needed. This product is an All-in-Mix pre-mixed formulation that already contains the key primer components needed for reverse transcription (such as Oligo(dT) and random primers).

This product’s buffer system has been optimized with a certain tolerance for common PCR inhibitors. However, using high-quality RNA templates (e.g., OD260/280 ratio between 1.8-2.0) is still recommended to ensure optimal reverse transcription efficiency and cDNA yield.

Yes. The cDNA synthesized by this product is suitable for both dye-based and probe-based qPCR detection. For probe-based multiplex qPCR, design and optimize the multiplex reaction system based on the specific qPCR instrument configuration and probe labeling requirements.

The product requires cold chain transport at <=0 degrees C. Long-term storage requires -20 degrees C. Avoid repeated freeze-thaw and prolonged room temperature placement to ensure enzyme activity and product stability.

Under strict storage at -20 degrees C and avoiding repeated freeze-thaw, please refer to the product packaging label for the specific expiration date.