
An optimized spin column workflow that turns PCR products / agarose gels into high-quality DNA ready for downstream applications.
| Number | Specifications | Catalog Price | Add To Cart |
|---|---|---|---|
| YC48001-200 | 200T | Online Consult | + |
YALEPIC® Fast PCR And Gel DNA Purification Kit adopts optimized buffer system and silica gel column purification technology, which can recover 100 bp – 20 kb DNA fragments from various concentrations of TAE or TBE agarose gel. In addition, the kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by other methods, and remove impurities such as proteins, other organic compounds, inorganic salt ions and oligonucleotide primers. It can ensure that the purification can be completed within 15 min. The purified DNA can be used directly for ligation, transformation, enzyme digestion, in vitro transcription, PCR, sequencing, microinjection, etc.
Various concentrations of TAE or TBE agarose gel.
PCR products, enzymatic reaction systems or crude DNA products.
Store at 10 ~ 30°C for one year and transport at room temperature.
It can stably purify and recover all DNA fragments in the 100 bp to 20 kb range, covering mainstream molecular biology experimental fragment sizes.
The entire process uses a rapid purification workflow, requiring only 15 minutes to complete binding, washing, and elution operations, making it highly efficient and time-saving.
Fully compatible, suitable for all conventional concentrations of TAE and TBE agarose gels with no system limitations.
Each spin column can bind up to 10 microgram of DNA, providing ample loading capacity to meet high-concentration sample purification needs.
Yes. Purified DNA has high purity, no impurities, and good integrity, and can be directly used for sequencing, ligation and transformation, in vitro transcription, and various other downstream experiments.
Under standard experimental conditions, DNA recovery rate can reach up to 80%, significantly higher than ordinary purification kits, with greater experimental tolerance.
It supports room-temperature shipping without cold chain. For daily storage, it can be kept sealed at 10-30 degrees C room temperature, making storage convenient.
It can be used not only for agarose gel DNA recovery, but also for purifying crude DNA from PCR products, restriction digestion, ligation, probe labeling, and various enzyme reaction systems.