YALEPIC^® 1 kb DNA Ladder

Designed for agarose gel electrophoresis, not recommended for PAGE. As a ready-to-use product pre-mixed with 1x Loading Buffer, load 5 microL directly per lane. For wider wells, increase to 10 microL for adequate band brightness.

Consists of 8 double-stranded DNA fragments: 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 8000 bp, 10000 bp. Band concentrations: 4000 bp at 100 ng/5 microL (bright indicator), 6000 bp at 40 ng/5 microL (slightly dimmer), remaining 6 bands at 50 ng/5 microL. This configuration facilitates both fragment size positioning and semi-quantitative analysis.

Recommended 1% agarose gel for effective separation of 1000-10000 bp range. For higher resolution in the low molecular weight region, increase to 1.5%-2%. Excessive concentration impedes large fragment migration; too low results in insufficient small fragment separation.

Possible causes: (1) Electrophoresis buffer overused – reduced ion strength and buffering capacity; use fresh TAE or TBE each time. (2) Excessive voltage (>20 V/cm) causing band diffusion. (3) Insufficient or excessive loading volume. (4) Mismatched agarose concentration and target fragment size. Follow the recommended 5 microL loading and 1% gel concentration, and control voltage appropriately.

Small fragment weakening is common because small DNA fragments migrate faster and may reach the dye front where dye-DNA binding may dissociate, reducing fluorescence. Recommendations: (1) Use fresh electrophoresis buffer with proper ion strength; (2) Appropriately shorten electrophoresis time or reduce voltage; (3) Add EB or equivalent dye to the gel at 0.5 microgram/mL; (4) Use a higher-resolution gel documentation system.

Not recommended. The molecular weight range (1000-10000 bp) achieves ideal separation in agarose gels. In non-denaturing PAGE, DNA fragments in this range exhibit significantly different migration behavior than in agarose, making band separation unreliable. Use a PAGE-optimized DNA Marker for polyacrylamide gels.

Store at 2-8 degrees C protected from light for 2 years; -20 degrees C for 3 years. Aliquot into small single-use portions after first use to avoid repeated freeze-thaw of the entire tube. Repeated freeze-thaw may cause DNA strand breakage or degradation, affecting band clarity and concentration accuracy. Working solution at 2-8 degrees C should be used within 4 weeks.

Prepared by restriction digestion of plasmid DNA, providing more accurate molecular weight positioning, cleaner background, and no non-specific band interference compared to traditional PCR product-mixed markers. Pre-mixed 1x Loading Buffer, ready to use. The 4000 bp super-bright indicator band (100 ng/5 microL) has extremely high visibility for quick positioning. YALI Biotech also offers highly competitive market pricing.

YALEPIC 1Kb DNA Marker undergoes strict nuclease inactivation during manufacturing and contains no residual nuclease activity. It serves only as an electrophoresis molecular weight reference and will not degrade co-loaded sample DNA. However, use dedicated pipette tips and loading buffers and follow standard electrophoresis practices to avoid cross-contamination.

Tailing or smiling effects are typically related to: (1) Uneven gel preparation or loading before complete solidification; (2) Buffer level not covering gel surface; (3) Unstable voltage or excessive current; (4) Overloading. Recommendations: ensure gel is fully solidified before removing comb; buffer should cover gel surface by 1-2 mm; maintain constant voltage 80-120 V; load 5 microL as recommended, avoid overloading.