An optimized spin column workflow that turns plasmid from larger E. coli culture into high-quality plasmid DNA ready for downstream applications.
| Number | Specifications | Catalog Price | Add To Cart |
|---|---|---|---|
| YC47006 | 50T | Online Consult | + |
YALEPIC® Ultrapure Plasmid Midi Isolation Kit purifies 1 ~ 1 5 ml of cultured bacteria . The kit utilizes a silica-based membrane technology , pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 40 µg of high-copy plasmid DNA.The purified plasmid DNA is ready for use in molecular applications such as enzyme digestion, PCR, sequencing, ligation, transformation.
1 ~ 15 ml of overnight bacterial culture.
For low-copy-number plasmids, it is recommended to increase the culture volume to ≤20 ml and proportionally increase the amounts of PA, PB, and PM2 Buffer reagents.
Store at 10 ~ 30°C for one year and transport at room temperature.
If ambient temperatures often exceed 30°C, we suggest storing RNase A at 2 ~ 8°C.
After addition of RNase A, Buffer PA is stable for 6 months when stored at 2 ~ 8°C.
Designed for 1-15 ml of overnight E. coli culture, compatible with routine experimental volumes without additional reagent ratio adjustments.
Standardized operation takes only 20 minutes. Maximum plasmid DNA yield per preparation can reach 100 micrograms, meeting routine experimental needs.
The kit includes a pre-mixed blue indicator that visually confirms cell lysis and neutralization completion through color changes, avoiding over-lysis or insufficient neutralization, reducing impurity contamination risk, and enabling visual operation.
Yes. The kit uses silica membrane purification technology to efficiently remove RNA, genomic DNA, and protein impurities. The extracted plasmid purity meets standards for direct use in restriction digestion, sequencing, PCR, in vitro transcription, and other downstream experiments without further purification.
No cold chain needed. Supports room temperature transport throughout. Store sealed at 10-30 degrees C upon receipt. Simple storage conditions without occupying refrigerator space.
Common causes include insufficient culture density, incomplete cell lysis, and inaccurate elution buffer application to the membrane center. Recommendations: use fresh culture with OD600 2.0-4.0, mix gently by inversion (avoid vigorous shaking), apply elution buffer to the silica membrane center and let stand 1 minute before centrifugation to significantly improve recovery.
The optimized formulation with specific silica membrane adsorption effectively removes RNA and genomic DNA impurities. Under normal operation, no significant residue is present, meeting standard molecular biology experiment requirements.
Production center: 4th floor, Building 9, Yangshan Science and Technology
Industrial Park,No. 8 Jinyan Road, Suzhou High-tech Zone
Marketing center: Pujiang High-tech Park, Minhang District, Shanghai
Copyright: 2026 YALI Biotech Co., Ltd.
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