YALEPIC^® Script IV First-Strand cDNA Synthesis Kit

Suitable for RT-PCR, RT-qPCR gene expression analysis, full-length cDNA cloning, RNA-seq library construction, hybridization, and other downstream applications. Particularly suitable for reverse transcription of RNA templates with complex secondary structures and low-abundance genes.

Script X Reverse Transcriptase is a novel variant engineered on the basis of M-MLV (RNase H-), with significantly improved thermal stability. It can effectively unwind complex RNA secondary structures at higher reaction temperatures, enhancing template affinity and processivity, while having higher impurity tolerance. Full-length cDNA synthesis capability reaches 15 kb, far superior to conventional M-MLV enzyme.

Wide template starting range: 1 pg to 5 microgram total RNA. Both trace samples and regular RNA samples are compatible, particularly suitable for low-abundance gene expression analysis.

Yes. The first-strand cDNA synthesis reaction mixture can be used directly as PCR or qPCR template without purification. It is recommended that the synthesis reaction mixture not exceed 1/10 of the total PCR reaction system to ensure optimal amplification efficiency.

Yes. Script X Reverse Transcriptase has high tolerance to common reverse transcription inhibitors (such as ethanol, guanidine salts, heparin, etc.). Even when RNA samples contain small amounts of impurities, efficient reverse transcription performance can be maintained, reducing RNA purification steps and saving time and cost.

Oligo(dT)20: specifically binds to the poly(A) tail of mRNA, suitable for analyzing mRNA expression and full-length cDNA synthesis. Random primers: randomly bind to multiple sites on the RNA template, suitable for RNA without poly(A) tails (such as bacterial RNA) or analyzing all regions of RNA. The kit provides Oligo(dT)20 and random primers as separate components. Users can flexibly choose or mix them based on experimental needs.

Through the optimized Script X Reverse Transcriptase, full-length cDNA up to 15 kb can be synthesized, suitable for long gene cloning and full-length transcript analysis.

Storage: long-term storage at -20 degrees C. Aliquoting is recommended to avoid repeated freeze-thaw. Transport: below 0 degrees C with ice packs or dry ice. When removing for use, handle on ice to prevent enzyme activity degradation.

The YALEPIC Script X series is a high cost-effectiveness universal first-strand cDNA synthesis kit, focusing on compatibility with a wide range of RNA templates and efficient reverse transcription of complex secondary structure RNA. The company also offers a one-step gDNA removal and first-strand cDNA synthesis kit for experimental needs requiring simultaneous removal of genomic DNA contamination. Users can select the appropriate product based on downstream application requirements.