Reverse Transcription

RT-PCR is a technique that combines reverse transcription (RT) of RNA with polymerase chain amplification (PCR) of cDNA. Firstly, cDNA is synthesized from RNA by reverse transcriptase, and then cDNA is used as template to amplify the synthesized target fragment. RT-PCR technology is sensitive and widely used. It can be used for detecting gene expression level in cells, RNA virus content in cells and directly cloning cDNA sequences of specific genes.