YALEPIC^® All-in-Mix 1st Strand cDNA Synthesis Kit (gDNA off)

YALEPIC^® All-in-Mix 1st Strand cDNA Synthesis Kit（gDNA off）provides a rapid, stable and efficient method of cDNA synthesis which is perfect for downstream two-step Real Time PCR. The RT MasterMix and the Reaction Mix contains all the required reagents of RT-PCR (include RT Enzyme, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture and Reaction Buffer), and a special heat-sensitive DNase to efficiently remove genomic DNA without interfering with cDNA. within 30 minutes across a broad temperature range (45–60°C). Integrated thermolabile dsDNase specifically eliminates double-stranded DNA while maintaining cDNA integrity, allowing synchronous genomic DNA removal and reverse transcription. Reactions proceed rapidly upon addition of only template RNA and nuclease-free water. The synthesized first-strand cDNA is immediately suitable for downstream applications including library construction, PCR, qPCR, and hybridization assays.

● High performance reverse transcription: 95% of RNA template could be reverse transcribed to cDNA.

● Good for complex template: This product can be used for the reverse transcription of RNA template which has complex secondary structure and high GC content.

● High compatibility for downstream analysis: This product could be co-used with many type of qPCR product for analysis with high sensitivity and stability.

Store at -20°C and transport at ≤0°C.