YALEPIC^® Ultrapure Plasmid Mini Isolation Kit (1-5ml)

YALEPIC^® Ultrapure Plasmid Mini Isolation Kit（1-5 ml）purifies 1 – 5 ml of cultured bacteria . The kit utilizes a silica-based membrane technology , pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 40 µg of high-copy plasmid DNA.The purified plasmid DNA is ready for use in molecular applications such as enzyme digestion, PCR, sequencing, ligation, transformation.

• Fast and Easy:  Simple and fast extraction from multiple samples within 30 min.
• High Purity: Extracted pure palsmid DNA can be used in most molecular applications.
• High Yields: Each column adsorbs up to 40 µg of plasmid DNA.

1 ~ 5 ml of overnight bacterial culture.

For low-copy-number plasmids, it is recommended to increase the culture volume to ≤10 ml and proportionally increase the amounts of PA, PB, and PM2 Buffer reagents.

Store at 10 ~ 30°C for one year and transport at room temperature.

If ambient temperatures often exceed 30°C, we suggest storing RNase A at 2 ~ 8°C.

After addition of RNase A, Buffer PA is stable for 6 months when stored at 2 ~ 8°C.