YALEPIC^® Endotoxin Free Plasmid DNA Maxi Isolation Kit (100-300ml)

YALEPIC^® Endotoxin Free Plasmid DNA Maxi Isolation Kit(100-300ml) is suitable for extraction from 100 – 300 ml of bacterial solution cultured overnight, using an improved SDS-alkaline lysis method to lyse the bacteria. Unique silica membrane adsorption technique to efficiently bind plasmid DNA. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the procedure. Endotoxin is removed on the column, which is fast and simple to use.  This kit can be used to rapidly extract 2 ~ 3 mg of pure high-copy plasmid DNA, with an extraction rate of 80% – 90%. The kit uses an unique process formula removes endotoxin, the content of endotoxin is extremely low and the cell transfection effect is excellent. The extracted plasmid could be directly used in enzyme digestion, PCR, in vitro transcription, transformation, sequencing and other molecular biology experiments.

Visual Procedure: solution PB (blue) indicates whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction.

Fast: the whole procedure can be performed within 50 min.

Low endotoxin residue: Extremely low endotoxin content (< 1 EU/μg DNA).

High yield: up to 3 mg.

100~300ml of overnight bacterial culture.

Store at 10 ~ 30°C for one year and transport at room temperature.

If ambient temperatures often exceed 30°C, we suggest storing RNase A at 2 ~ 8°C.

After addition of RNase A, Buffer PA is stable for 6 months when stored at 2 ~ 8°C.