YALEPIC® Tryzol Plus Columnar Isolation Kit(No DNase I)is applicable for isolating high-quality total RNA from various samples such as cultured cells, animal and plant tissues. This kit combines phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA, with the advantages of strong lysing ability, wide compatibility, fast, high yields, and good purity. Samples are fully lysed with Tryzol Reagent,and RNA is specifically adsorbed from the aqueous phase by silica membrane. The obtained total RNA can be directly used for molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.