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YALEPIC® DNA/RNA Transfection Reagent-LFDR3+

Number Specifications Catalog Price
YJ52011-01 1ml Online Consult

Product Description

YALEPIC® DNA/RNA Transfection Reagent-LFDR3+ is a liposome-mediated transfection reagent. It is applicable for plasmid DNA and RNA transfection of various adherent or suspension cells. With a clear structure, LFDR3+ Reagent can achieve efficient and highly reproducible cell transfection. Enhancer Reagent, as a transfection reagent enhancer, can assist DNA to enter the nucleus quickly. The combination of two reagents can achieve higher transfection efficiency for large fragment plasmids and difficult-to-transfect cells. LFDR3+ has the excellent ability to form liposome/nucleic acid complexes and rapid release of nucleic acid transfected into cells, which ensures excellent transfection performance and extremely low cytotoxicity. The presence of serum and antibiotics does not affect its transfection effect, so the formed liposome/nucleic acid complex can be directly added to the complete medium.

Features

  • Superior performance: Efficient transfection of large plasmids and difficult-to-transfect cells.
  • Broad compatibility: Only one reagent can be used for transfection of plasmid DNA, mRNA, siRNA, and co-transfection, etc.
  • Low cytotoxicity: No need to change the medium after transfection.

Storage

Store a 2 ~ 8°C. Adjust the shipping method according to the destination.Do not freeze.

1.LFDR与DNA/siRNA的比例不合适。

1)LFDR(μl):DNA(μg) 剂量可以在1:1和5:1μl 之间进行调整。

2)LFDR(μl):siRNA(pmol)剂量可以在0.02:1和0.15:1之间进行调整。

2.转染时细胞密度不合适。

细胞密度过低导致细胞生长缓慢,对外来刺激变得较为敏感,使得转染毒性增高。细胞密度过高,会导致细胞发生接触抑制,加快细胞凋亡。细胞融合度达到60% – 70%时,转染可以取得较高的转染效率。可预实验优化细胞转染密度。

3.转染体系中存在抑制因素。

当转染体系中存在多聚阴离子聚合物时(例如:硫酸葡聚糖、肝素等),转染将无法正常进行,因此应避免上述物质存在于细胞转染体系中。

4.细胞状态差。

应尽量使用适度传代接种的细胞系,并尽量在平行实验时保持细胞传代次数的一致性,同时需避免细胞培养时间过长。

5.细胞毒性高。

导致转染时细胞毒性大的因素有很多,例如DNA的用量过大、转染试剂的用量过大、转染时细胞状态较差以及培养基中抗生素的加入等。建议严格按照所选择转染试剂的说明书进行操作,以避免细胞毒性大的问题。

6.转染效果不稳定

一个是转染试剂的稳定性,另一个是基因的稳定性。转染试剂应按照说明书建议的保存温度及条件进行保存。核酸如短时间内连续使用,可放置于4℃保存。若超过10天不使用,建议置于-20℃或-80℃长期储存以降低核酸的降解速度。

7.DNA-siRNA共转染时,基因沉默效率不够高

当细胞同时转染DNA和siRNA时,建议使用分别转染的方式进行。即使用基因转染试剂分别与DNA和siRNA进行复合,再将两个复合物体系分两次分别加入到同一个装有细胞的实验孔中。

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