YALEPIC® Ultrapure Plasmid DNA Maxi Isolation Kit(100-300ml) is suitable for extraction from 100 – 300 ml of bacterial solution cultured overnight, using an improved SDS-alkaline lysis method to lyse the bacteria. This kit can be used to extract 2 ~ 3 mg of pure high-copy plasmid DNA, with an extraction rate of 80% – 90%. The extracted plasmid could be directly used in enzyme digestion, PCR, in vitro transcription, transformation, sequencing and other molecular biology experiments.