Complete Nipah Virus Detection Workflow – From Sample Collection to qRT-PCR Results

With Kerala experiencing its ninth Nipah outbreak since 2018 and mortality rates reaching 100% , India’s need for rapid, reliable detection capabilities has never been more urgent. Recent surveillance shows Nipah antibodies in fruit bats across multiple states, indicating widespread potential for future outbreaks.

Yali Biotech – Complete Nipah Virus Detection Solution
As a leading biotech company, we offer a comprehensive, integrated workflow specifically designed for Nipah virus detection, from sample collection to final qRT-PCR results.

Besides the conventional “collect-extract-detect” pipeline, we now offer an EMERGENCY-SPEED PROTOCOL tailored for acute, high-mortality Nipah virus infections:

1. Collect & Lyse in one step
Transfer swab into YALEPIC^® Viral Nucleic Acid Storage Tube (Direct) （YS21113）
– Instant virus inactivation
– Complete lysis in 2 min at room temperature
– Zero inhibitory components – lysate can go straight into RT-qPCR

2. Direct amplification – NO extraction
Use 2–5 µL crude lysate as template, add YALEPIC^® U+ One Step qRT-PCR Master Mix (Probe) (YPQ42003)
– UNG plus dUTP prevents carry-over contamination
– Detects RNA templates down to 0.1 pg, accurately capturing low-level infections.
– Co-detect multiple Nipah genes

If you prefer the conventional “collect-extract-detect” pipeline, we also offer a COMPLETE, VALIDATED SAMPLE-TO-RESULT WORKFLOW.

STEP 1: Sample Collection & Preservation
YALEPIC^® Viral Nucleic Acid Storage Tube (YS21112)

Room temperature stability: Prevents degradation during transport/storage
Prevents contamination: Single-use, sterile collection system
Cost-effective: No cold chain required
We also offer storage solutions for blood, urine, bronchoalveolar fluid, sputum, swabs, stool, CSF, cervical cells, tissue.

STEP 2: Nucleic Acid Extraction
Magnetic Bead-Based Extraction (High Throughput)
YALEPIC^® MagEVO Viral DNA/RNA Extraction Kit (YG28002) (https://www.yalibioglobal.com/product/359.html)
Automated compatible
YALEPIC^® Auto-Pure 32 Nucleic Acid Extraction System (MC-A-32-16) (https://www.yalibioglobal.com/product/1535.html)

YALEPIC^® Auto-Pure 96 Nucleic Acid Extraction System (MC-A-96-S) (https://www.yalibioglobal.com/product/1537.html)

STEP 3: qRT-PCR Detection
Probe-Based Detection (Recommended for Nipah)
YALEPIC^® U+ One Step qRT-PCR Master Mix (YPQ42003)
Ultra-sensitive: Detects RNA templates down to 0.1 pg, accurately capturing low-level infections.
Multiplex capability: Co-detect multiple Nipah genes.
UNG plus dUTP protection: Prevents carryover contamination.

ADDITIONAL LABORATORY CONSUMABLES
PCR tubes, PCR plates, PCR plate sealing film, pipette tips… (https://www.yalibioglobal.com/product_category/pcr-consumables)

WHY CHOOSE YALI BIO FOR INDIA?
✓ Complete solution
✓ Proven performance
✓ Cost-effective
✓ Regulatory ready
✓ Technical support
✓ Rapid deployment

IMMEDIATE NEXT STEPS
We invite you to:
Request product samples for evaluation
Schedule technical consultation with our experts
Discuss distribution partnership opportunities
Review regulatory pathway for Indian market entry
Contact us today – Time is critical for India’s Nipah preparedness.