YALEPIC^® Single-Cell WGA Kit

It supports single living cells, embryo biopsy TE cells, trace amounts of peripheral blood, 10 pg-ng level trace genomic DNA, small amounts of FFPE dissociated single cells. A conventional single cell can stably amplify 2-5 microgram of genomic DNA.

The standard protocol completes all amplification in 3 hours: conventional lysis 30 min + pre-amplification + formal amplification. It is not recommended to arbitrarily shorten the reaction time, as this will reduce genome coverage.

Samples after cell lysis completion can be transported at room temperature at temperatures <=37 degrees C for short distances without compromising DNA integrity. This is a core advantage distinguishing this kit from conventional WGA products on the market. For long-distance transport, refrigeration is still recommended.

The product purity meets NGS library preparation requirements and can be directly used for whole-genome sequencing, exon capture sequencing, CNV copy number variation detection, SNP genotyping, and other downstream experiments.

It uses a directed-evolution optimized Phi29 polymerase with high fidelity and strong strand displacement activity, achieving lower amplification bias and whole-genome coverage >=95%, superior to conventional wild-type Phi29 materials.

The complete set includes lysis buffer, pre-amplification buffer, Phi29 enzyme, amplification buffer, and other full reaction reagents. No additional purchase of配套 components is needed – ready for use directly on the instrument.

Stock solutions must be stored at -20 degrees C protected from light. Repeated freeze-thaw or prolonged room temperature storage will cause enzyme activity degradation, resulting in low amplification yield and reduced coverage.

Amplification products should be aliquoted and stored at -20 degrees C for short-term (1 month) or at -80 degrees C for long-term (2 years). Repeated freeze-thaw should be <=3 times.

Usually caused by consumable or workbench exogenous DNA contamination. Replace with sterile, nuclease-free centrifuge tubes, UV-sterilize the workbench, and open a fresh negative control buffer to eliminate contamination.

Insufficient cell lysis, starting cell degradation, or reaction temperature fluctuation. Optimize lysis incubation time and add enzymes on ice throughout the process to improve product yield.

The optimized buffer is compatible with animal/human single cells. Plant cell walls are thick and require pre-optimization of the lysis protocol. A small number of plant samples can be validated through pilot experiments.

The reagent performance has been validated with PGT samples and can be used for blastocyst biopsy single-cell amplification and embryo chromosome CNV screening.

Core advantage comparison: Enzyme type – YALEPIC uses directed-evolution Phi29 enzyme with superior uniformity; conventional kits use wild-type Phi29 with higher bias. Coverage – YALEPIC >=95%; conventional typically 80%-90%. Amplification time – YALEPIC 3 hours; conventional 4-8 hours. Transport method – YALEPIC allows room temperature transport after lysis at <=37 degrees C; conventional typically requires full -20 degrees C cold chain. Product yield - YALEPIC 2-5 microgram; conventional 1-3 microgram. Application scenarios - YALEPIC supports WGS/WES/PGT/CNV/qPCR, etc.; conventional mostly limited to qPCR/genotyping. The YALEPIC single-cell WGA kit is a new-generation single-cell WGA solution that balances high coverage, high uniformity, operational convenience, and transport flexibility.