YALEPIC^® 8K DNA Ladder

Yes, this is a ready-to-use product, pre-mixed with 1x Loading Buffer. Simply load 5 microL directly for agarose gel electrophoresis – very convenient and quick.

This product consists of 10 double-stranded DNA fragments: 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, 5000 bp, 8000 bp. The 750 bp band is at 100 ng/5 microL (bright band); 1500 bp at 40 ng/5 microL; all other bands at 50 ng/5 microL.

This product is suitable for agarose gel electrophoresis, not recommended for polyacrylamide gel electrophoresis (PAGE). For high-resolution double-stranded DNA analysis in polyacrylamide gels, use a dedicated PAGE DNA Marker.

Store at 2-8 degrees C for 2 years, or -20 degrees C for 3 years. Avoid repeated freeze-thaw. Do not heat. Transport with ice packs.

Common causes and solutions: (1) Stale electrophoresis buffer – reduced ion concentration and elevated pH cause blurry bands; replace with fresh buffer. (2) Improper gel preparation – ensure agarose is fully dissolved; allow gel to set completely (30-60 minutes). (3) Improper loading volume – try diluting 2-3x with water and loading 8-10 microL.

Yes, compatible with GelRed, SYBR Green, EB, and other common DNA fluorescent dyes in standard agarose gel electrophoresis staining systems.

Band degradation or tailing is typically caused by nuclease contamination. Use nuclease-free pipette tips and centrifuge tubes for aliquoting, avoid repeated freeze-thaw, and maintain aseptic technique during operation.

This product is prepared by restriction digestion of plasmid DNA, providing higher molecular weight accuracy, clearer and sharper bands, cleaner background, and excellent batch-to-batch stability compared to PCR product-mixed markers. The ready-to-use pre-mixed Loading Buffer design also saves preparation time.

Not recommended. DNA Markers are double-stranded DNA molecules; in RNA denaturing gel electrophoresis, double-stranded structures will denature, making size indication inaccurate. For RNA molecular weight reference, use a dedicated RNA Marker. However, for rough observation in standard agarose gel electrophoresis, it can be used approximately.

Suitable for PCR product identification, restriction fragment size analysis, genomic DNA fragment estimation, plasmid DNA identification, and other common molecular biology experiments. Covers a wide molecular weight range of 100-8000 bp, meeting most routine DNA fragment analysis needs.