YALEPIC^® 2K DNA Ladder

This product is designed for agarose gel electrophoresis, not recommended for PAGE. Under 1% agarose gel at 6-8 V/cm for 20-40 minutes, band separation is optimal. PAGE has higher resolution but different DNA migration characteristics, potentially leading to inaccurate fragment size determination.

This is a ready-to-use product, pre-mixed with 1x Loading Buffer. Load 5 microL directly per electrophoresis lane – no additional preparation needed. For smaller gels or narrow wells, adjust to 2-3 microL as needed.

The 750 bp band is designed as an enhanced reference band at 100 ng/5 microL, while other bands are 50 ng/5 microL. This design facilitates quick reference band identification and semi-quantitative analysis of target DNA fragments. For accurate quantification, compare target band brightness with the 750 bp enhanced band.

Common causes and solutions: Inappropriate agarose concentration – use 1% gel; too high or low affects resolution. Uneven gel or excessive bubbles – re-prepare gel, ensure complete dissolution and no bubbles. Excessive voltage – use moderate 6-8 V/cm to avoid heat-induced band diffusion. Repeated freeze-thaw degradation – aliquot to avoid. Excessive loading – maintain 5 microL; excess causes band spreading or cross-contamination between lanes.

Both have identical band composition (100bp/250bp/500bp/750bp/1000bp/2000bp, 6 bands), similar specifications and ready-to-use design. Main differences: Manufacturing process – YALEPIC 2K uses restriction digestion of plasmid DNA, providing higher band clarity and batch stability. Brand positioning – YALI Biotech offers a more cost-effective option. They can be used interchangeably without protocol adjustments.

Store at 2-8 degrees C for 24 months, or -20 degrees C for 36 months. Choose based on usage frequency: frequent use – store at 4 degrees C; long-term unused – aliquot and store at -20 degrees C to avoid repeated freeze-thaw DNA degradation.

Yes, for semi-quantitative analysis. The 750 bp enhanced band is at 100 ng/5 microL, others at 50 ng/5 microL. Estimate target DNA concentration by comparing band brightness between sample and Marker lanes. For precise quantification, use fluorometric methods (Qubit or qPCR).

Common situations: All bands dim – electrophoresis time too long causing diffusion, or insufficient dye staining. Only bottom two bands bright, upper bands weak – Marker degradation; replace with fresh Marker. Uneven brightness – 750 bp being significantly brighter is normal; if other bands show abnormal brightness differences, check gel preparation uniformity and electrophoresis conditions.