YALEPIC^® 100 bp Plus DNA Ladder

The YALEPIC 100bp Plus DNA Marker has a wider band coverage (100-2000 bp, 10 bands) compared to the regular 100bp Marker (100-1000 bp). The 500 bp band is designed as an enhanced bright band (100 ng/5 microL) for quick positioning and semi-quantitative analysis. Additionally, the product is pre-mixed with 1x Loading Buffer, ready to use.

Tailing or blurring is typically caused by: Stale electrophoresis buffer – replace with fresh 1x TAE or TBE. Inappropriate gel concentration – use 1-1.5% agarose for 100bp Marker. Excessive loading volume – use 5 microL per lane as recommended. DNA degradation – avoid nuclease contamination, use nuclease-free consumables. After multiple uses, buffer ion strength decreases and pH rises, causing blurry or irregular migration – use fresh buffer each time.

Normal. The 500 bp band is specifically designed as an enhanced bright band (100 ng/5 microL), approximately 2x the concentration of other bands (~50-40 ng/5 microL). This design provides a clear reference for quick positioning and accurate quantification. Other bands are slightly dimmer but clearly visible. This is a widely adopted design in similar products.

Not recommended. This product is designed and optimized for agarose gel electrophoresis, not recommended for polyacrylamide gel electrophoresis (PAGE). For PAGE analysis, use a specialized non-denaturing PAGE DNA Marker. This product also cannot be used for RNA analysis in denaturing gels, as double-stranded DNA will denature to single strands under denaturing conditions, making it inaccurate for RNA molecular weight indication.

YALEPIC 100bp Plus DNA Marker supports two storage methods: 2-8 degrees C refrigerated for 2 years, -20 degrees C frozen for 3 years. Practical recommendations: Frequent use (weekly): store at 2-8 degrees C for convenience. Long-term storage (backup stock): store at -20 degrees C to maximize shelf life. Thaw and mix thoroughly before use. Avoid repeated freeze-thaw; aliquot for best stability.

Compatible. The YALEPIC 100bp Plus DNA Marker works with common nucleic acid fluorescent dyes including GelRed, SYBR Green, and GoldView. After electrophoresis, observe under the corresponding excitation light in a gel documentation system. Mix DNA Marker with dye before loading, or stain the gel post-electrophoresis – both methods produce clear band images.

This is a ready-to-use product, pre-mixed with 1x Loading Buffer – no additional addition needed. The optimized Loading Buffer formula ensures DNA samples sink into the well during electrophoresis and forms a clear tracking front for monitoring electrophoresis progress.

Common causes and solutions: Inappropriate agarose concentration – use 1%-1.5% for 100-2000 bp fragments; too low reduces small fragment resolution, too high slows electrophoresis. Uneven gel preparation – ensure agarose is completely dissolved and mixed before pouring. Excessive voltage – causes gel heating and reduces resolution; maintain 5-8 V/cm. Improper loading volume – use exactly 5 microL; excess causes band tailing. Use freshly prepared gels and regularly replace electrophoresis buffer for best separation.