Pathogenic microorganisms span viruses, bacteria, and fungi — each with distinct nucleic acid types (DNA or RNA), cellular structures, and lysis resistance. In real-world clinical settings, co-infections are common: a single patient may carry both bacterial and viral pathogens simultaneously. An extraction kit must offer broad-spectrum compatibility to avoid missed detections.
Influenza A/B, SARS-CoV-2, Adenovirus, HBV, CMV, EBV — nucleic acid can be DNA or RNA, requiring simultaneous co-extraction.
Single-stranded & Double-stranded
Staphylococcus, Streptococcus pneumoniae, E. coli, Salmonella, Shigella, Mycobacterium tuberculosis. Gram-positive bacteria have thick peptidoglycan walls resistant to lysis.
Hard-to-Lyse: G+ Species
Candida albicans, yeasts, Aspergillus, Cryptococcus. Cell walls contain chitin and glucan, making conventional lysis methods ineffective for nucleic acid release.
Chitin-Rich Cell Wall
⚠ The Core Challenge: Traditional extraction protocols often require separate kits for different pathogen types, increasing costs and risking detection omissions. Simultaneous co-extraction of viral, bacterial, and fungal DNA and RNA in a single tube is the critical requirement for modern pathogen diagnostics.
Before selecting an extraction solution, it is essential to understand the technical barriers that compromise downstream accuracy.
Hemoglobin in whole blood, mucin in sputum, polysaccharides and bile salts in stool — all are potent PCR inhibitors. Insufficient purification leads to severely suppressed qPCR signals and false negatives.
G+ bacteria (Staphylococcus, Streptococcus) have thick peptidoglycan; fungi (Candida, yeasts) contain chitin. Standard lysis buffers fail to disrupt these structures, resulting in low nucleic acid yield and poor detection of low-abundance pathogens.
Clinical samples (especially blood and BALF) contain massive amounts of human genomic DNA; pathogen nucleic acid represents only trace amounts. Without effective host DNA reduction, pathogen signal in mNGS is completely overwhelmed.
DNA viruses (HBV, CMV) and RNA viruses (influenza, SARS-CoV-2) require separate extraction workflows, increasing hands-on time and potentially causing sample insufficiency or incomparable results from split aliquots.
Phenol-chloroform extraction uses hazardous organic solvents endangering lab staff. Spin-column methods offer limited throughput, making them inadequate for CDC and hospital labs processing hundreds of samples daily.
The YALEPIC MagEVO Pathogen DNA/RNA Extraction Kit (Cat# YG28001) was purpose-built for clinical pathogen detection and microbiome sequencing research. Through an optimized lysis buffer + high-affinity silica-based magnetic bead formulation, it simultaneously isolates and purifies viral, bacterial, and fungal DNA and RNA from diverse biological samples — completely free of phenol, chloroform, and other toxic organic reagents.
Each feature is engineered to solve a specific bottleneck in pathogen detection workflows.
Simultaneously supports G+/G− bacteria, fungi, and both DNA and RNA viruses. One kit solves the co-infection detection challenge.
Specific magnetic bead binding with optimized wash buffer. A260/A280 consistently 1.8–2.0. Low-abundance pathogen detection rate improved by over 30%.
Works with all major magnetic-rod and pipetting-based automated workstations. Standardized 96-sample-per-batch processing, minimizing manual variation.
Non-toxic or low-toxicity lysis formulation replaces traditional phenol-chloroform. Protects lab personnel and aligns with green lab standards.
Manufactured to ISO-quality standards. Inter-batch CV <5%, ensuring long-term data comparability and full traceability across monitoring programs.
10–30 °C storage, 18-month shelf life. No cold chain required — dramatically cuts shipping and warehousing costs.
MagEVO’s three-step strategy is engineered to overcome the most stubborn pathogen cell walls while preserving nucleic acid integrity.
Combines chemical lysis with mechanical disruption, optimized for thick-walled fungi (yeasts, Candida) and Gram-positive bacteria. The optimized lysis buffer simultaneously disrupts viral capsids, bacterial cell walls, and fungal cell walls for maximum nucleic acid release.
Surface-modified silica magnetic beads with high affinity and specificity for nucleic acids. Under optimized buffer conditions, beads selectively bind DNA/RNA while PCR inhibitors — hemoglobin, mucin, polysaccharides — remain in the liquid phase and are removed.
Multi-step wash with optimized buffers further removes residual inhibitors and host nucleic acid background. The final product achieves A260/A280 = 1.8–2.0, free of PCR inhibitors and nucleases, ready for downstream applications.
The kit is validated across five major clinical and research sample categories, covering the most common specimen types encountered in infectious disease diagnostics.
Whole blood, plasma, serum
Nasal/pharyngeal swabs, sputum, BALF
CSF, pleural/ascitic fluid, joint fluid
Stool, urine
Tissue homogenates, microbial cultures
MagEVO-extracted nucleic acids are validated for the full range of downstream molecular biology workflows.
Pathogen nucleic acid detection and quantification — HBV viral load, respiratory multi-pathogen screening panels.
Unknown pathogen identification, co-infection profiling, antimicrobial resistance gene detection.
Bacterial 16S rDNA amplification, fungal ITS region amplification, pathogen species identification.
De novo pathogen genome assembly, variant analysis, molecular epidemiological source tracing.
16S rDNA amplicon sequencing, shotgun metagenomics for gut microbiota community analysis.
Clinical isolate characterization, environmental microbial community analysis.
Gram-positive bacteria (M. tuberculosis, S. aureus) and fungi (Candida, Aspergillus) — classified as “hard-to-lyse” pathogens — pose the greatest extraction challenge. MagEVO’s chemical lysis + mechanical disruption dual strategy is purpose-built for these difficult organisms.
As sample volumes grow, manual extraction is no longer scalable. MagEVO is validated across major open and closed magnetic-rod extraction instruments with excellent performance.
Whether operator A on Monday or operator B on Friday — results are highly reproducible and consistent.
Multiple respiratory pathogen targets verified: smooth amplification curves, standard curve linearity R² > 0.99, no amplification in negative controls. Complex inhibitor-rich samples show excellent amplification efficiency with Ct values advancing by 1.5–2.0 cycles on average.
In a suspected CNS infection case, MagEVO-extracted nucleic acids successfully detected low-abundance Listeria monocytogenes sequences, while the control extraction method failed. Host sequence proportion was significantly reduced; pathogen coverage and depth met established clinical standards.
Extracted RNA demonstrates excellent RIN values, meeting the requirements for reverse transcription and cDNA synthesis. This is critical for RNA virus detection (influenza, coronavirus) and transcriptome analysis.
Prioritize automation compatibility, room-temperature stability, and clinical sample validation track record. MagEVO’s 96-sample/run throughput with CV <5% meets the demands of high-volume, multi-target diagnostic operations.
For hard-to-lyse pathogen research or metagenomic sequencing, focus on lysis efficiency and inhibitor removal capability. MagEVO’s >85% recovery rate for tough organisms ensures the depth and breadth required for publication-grade data.
YALI BIOTECH is a National High-Tech Enterprise integrating R&D, manufacturing, and sales, headquartered in Yangshan Science & Technology Park, Suzhou New District. Driven by technological innovation, the company focuses on the industrialization of molecular detection technologies with hundreds of R&D reserves across sample preservation, nucleic acid extraction & purification, and molecular detection enzyme raw materials — delivering professional, high-performance products for life sciences, in-vitro diagnostics, biomedicine, health testing, and inspection & quarantine.
YALI BIOTECH’s product portfolio spans eight major categories: Sample Collection & Preservation, Nucleic Acid Extraction & Purification, Molecular Biology Reagents, High-Throughput Sequencing, Cell Culture & Assay, Laboratory Instruments, Lab Consumables, and Medical Devices — with customizable OEM/ODM services available.
Flagship products include the YALEPIC MagEVO Pathogen DNA/RNA Extraction Kit, YALEPIC Pure 32 Automated Nucleic Acid Extractor, and YALEPIC sample preservation series for blood, tissue, and urine. These solutions are trusted by CDCs, hospital clinical labs, third-party diagnostic centers, and academic research institutions worldwide.
In pathogen diagnostics — a field directly impacting health and safety — a stable, efficient, and safe extraction solution is the most trusted partner for researchers and clinical laboratory professionals.
🌐 Learn more at www.yalibiotech.com
Yes. YALEPIC MagEVO Pathogen DNA/RNA Extraction Kit (YG28001) uses an optimized lysis buffer and high-affinity silica-based magnetic beads to co-extract DNA and RNA from Gram-positive/negative bacteria, fungi, and both DNA and RNA viruses in a single tube, eliminating the need for separate kits.
MagEVO employs a dual strategy of chemical lysis plus mechanical disruption. The lysis buffer contains strong detergents and chelating agents that soften cell wall structures. Verified data shows nucleic acid recovery rates exceeding 85% for yeast and Bacillus subtilis, compared to under 40% with conventional reagents, with A260/A230 ratios above 2.0.
Absolutely. MagEVO is validated on mainstream open and closed magnetic-rod-based nucleic acid extraction instruments. It supports high-throughput processing of 96 samples per batch with CV below 5% across 10 consecutive runs. Pre-filled plate designs simplify setup and reduce operator error.
YALEPIC MagEVO is stored at 10–30 °C (room temperature) with an 18-month shelf life. No cold chain, dry ice, or refrigerated shipping is required, significantly reducing logistics costs and eliminating the risk of reagent degradation from cold-chain breaks.
The kit supports five major categories: blood (whole blood, plasma, serum), respiratory (nasal/pharyngeal swabs, sputum, BALF), body fluids (CSF, pleural/ascitic fluid, joint fluid), excreta (stool, urine), and other samples including tissue homogenates and microbial cultures.
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Industrial Park,No. 8 Jinyan Road, Suzhou High-tech Zone
Marketing center: Pujiang High-tech Park, Minhang District, Shanghai
Copyright: 2026 YALI Biotech Co., Ltd.
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