YALEPIC^® Trizol Plus RNA Extraction Kit (DNase I-Free)

YALEPIC Tryzol Plus features two key optimizations over the traditional Trizol method: First, it uses TryCOM as a chloroform substitute for phase separation, dramatically reducing toxic reagent exposure risk and making operation safer and more environmentally friendly. Second, the kit includes a dedicated RNA dissolution buffer, ensuring that extracted RNA dissolves fully and has better stability. The overall lysis principle and workflow remain consistent with the classic Trizol method, enabling seamless switching from existing laboratory Trizol extraction protocols without the need to re-optimize experimental conditions.

This kit is primarily designed for total RNA extraction. During the phase separation step, RNA partitions into the upper aqueous phase, while DNA and protein reside in the interphase and lower organic phase, respectively. If needed, users can separately collect the interphase and lower organic phase and recover genomic DNA and protein through isopropanol/ethanol precipitation methods, enabling simultaneous recovery of RNA, DNA, and protein from a single sample, ideal for comprehensive utilization of precious samples.

Total RNA extracted with the YALEPIC Tryzol Plus kit shows high purity, with A260/A280 ratios typically stable between 1.9 and 2.1, with almost no protein or genomic DNA contamination. It can be directly used for downstream experiments with high RNA purity requirements such as RT-PCR, Real-Time PCR, Northern Blot, and RNA-Seq without additional purification steps.

TryCOM has far lower toxicity and volatility than traditional chloroform, significantly improving usage safety. Routine operations can be performed on a standard lab bench without mandatory fume hood use. However, since Tryzol Reagent itself contains guanidine isothiocyanate and phenol components, it is still recommended to wear disposable gloves, a mask, and goggles throughout the operation and maintain basic laboratory ventilation.

The YALEPIC Tryzol Plus RNA Extraction Kit has a very broad application range and has been successfully applied to total RNA extraction from various sample types including animal tissues (liver, brain, muscle, etc.), cultured cells (adherent/suspension cells), plant tissues (leaves and other materials with low secondary metabolites), microorganisms (bacteria, yeast), viral samples, and body fluids (blood, serum). For plant samples with high polysaccharide/polyphenol content (e.g., mature fruits, bark), YALI BIOTECH’s plant-specific RNA extraction kit CAT#YR23016 is recommended for better results.

Completing total RNA extraction for a batch of samples with this kit takes approximately 30-60 minutes (depending on sample number). The main steps are: sample lysis -> add TryCOM for phase separation -> centrifuge and collect aqueous phase -> isopropanol precipitation of RNA -> ethanol wash -> dissolve RNA. The workflow is essentially the same as the classic Trizol method, requires no special equipment other than a standard benchtop centrifuge, has a low learning curve, and is suitable for both novice and experienced researchers.

This kit (CAT#YR23014) does not include DNase I because the optimized phase separation process yields total RNA with extremely low genomic DNA carryover, which is directly usable for most routine RT-PCR and qPCR experiments. If your downstream experiments have stringent DNA carryover requirements (e.g., RNA-Seq library construction, low-abundance gene detection), it is recommended to additionally use YALI BIOTECH’s DNase I products (e.g., gDNA removal kit) for DNA digestion to ensure experimental accuracy.

Dissolve the extracted total RNA in the kit’s RNA dissolution buffer or Nuclease-free Water, aliquot, and store at -80 degrees C, which can preserve it effectively for 6-12 months. For short-term use (within 1-2 weeks), -20 degrees C storage is acceptable. Avoid repeated freeze-thaw; aliquot as needed. Keep dissolved RNA on ice during operation to avoid RNase degradation.

YALEPIC Tryzol Plus is a domestically developed RNA extraction kit by YALI BIOTECH. While maintaining extraction performance and quality comparable to imported equivalent products, its price is only 1/3 to 1/2 of imported products. The 100 mL specification catalog price is only 350 CNY, sufficient for approximately 100 routine sample extractions (approximately 1 mL per use), with a per-extraction cost as low as approximately 3.5 CNY, making it highly suitable for large-volume routine use in research laboratories, effectively reducing consumable costs.

You can inquire online through the YALI BIOTECH official website, or contact our sales team by calling the service hotline at 0512-66256010 or emailing info@yalibiotech.com. This kit supports bulk purchasing with exclusive volume discounts. YALI BIOTECH also provides OEM/ODM customization services to prepare different packaging specifications or special formulations according to customer needs. Research institutes, biotech companies, and distributors are welcome to inquire about cooperation.