An optimized Trizol + spin column workflow that turns cells and tissues into high-quality total RNA ready for downstream applications.
| Number | Specifications | Catalog Price | Add To Cart |
|---|---|---|---|
| YR23009-C | 100T | Online Consult | + |
YALEPIC® Tryzol Plus Columnar Isolation Kit（DNase I）is applicable for isolating high-quality total RNA from various samples such as cultured cells, animal and plant tissues. This kit combines phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA, with the advantages of strong lysing ability, wide compatibility, fast, high yields, and good purity. Samples are fully lysed with Tryzol Reagent,and RNA is specifically adsorbed from the aqueous phase by silica membrane. The obtained total RNA can be directly used for molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.
It is effectively isolated from a variety of sample sources (cells, animal tissues, fresh blood, and plant tissues).
DNase I transport and storage at -20℃ for one year.
Tryzol Reagent at 2 ~ 8°C and protect from light , Others at room temperature (10℃- 30℃) in a dry place for one year. Transport at room temperature.
Yes. The kit includes DNase I and its matching buffer for in-situ enzymatic removal of genomic DNA during the purification workflow, without additional treatment, effectively preventing DNA interference in downstream experiments.
No. This kit uses a TryCOM proprietary component instead of chloroform, containing no toxic organic solvents. Operation is safer and suitable for routine laboratory and teaching environments.
It is suitable for a wide range of sample types including animal tissues, cultured cells, microorganisms, plant materials with low secondary metabolites, viruses, and body fluids, offering strong versatility.
It can be directly used for RT-PCR, qPCR, gene chips (microarray), Northern Blot, Poly(A) selection, in vitro translation, molecular cloning, and other downstream molecular biology experiments.
DNase I and 10x DNase Buffer should be stored at -20 degrees C; Tryzol Reagent at 2-8 degrees C protected from light; all other components at room temperature. Strict adherence to storage conditions ensures stable shelf life.
This kit is better suited for plant materials with low secondary metabolite content. For complex plant samples rich in polysaccharides and polyphenols, YALI BIOTECH’s dedicated plant RNA extraction kit is recommended.
The spin column method is simpler and faster to operate with better reproducibility, more stable RNA purity and yield, and eliminates the need for isopropanol precipitation, reducing loss and contamination risk.
Routine animal tissue: approximately 50-100 mg; cultured cells: approximately 10^6-10^7 cells; plant tissue: <=100 mg. Refer to the product manual for specific optimization.
Production center: 4th floor, Building 9, Yangshan Science and Technology
Industrial Park,No. 8 Jinyan Road, Suzhou High-tech Zone
Marketing center: Pujiang High-tech Park, Minhang District, Shanghai
Copyright: 2026 YALI Biotech Co., Ltd.
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