YALEPIC^® Stool DNA Sample Storage Tube

No significant changes will occur. The design goal of this product’s preservation fluid is to stabilize stool microbial DNA at room temperature (<=37 degrees C) for at least 2 years. Experimental validation shows that within 2 weeks of room temperature storage, the relative abundance of major bacterial phyla (such as Firmicutes, Bacteroidetes, and Proteobacteria) shows no significant difference from -80 degrees C frozen control samples (p>0.05), making it highly suitable for multi-center cohort studies requiring long-distance shipping or inability to process immediately.

Absolutely. YALEPIC preservation fluid protects DNA integrity, preventing target gene degradation during storage. Combined with an optimized stool DNA extraction kit, the extracted DNA can be directly used for absolute quantitative qPCR, ddPCR, or quantitative PCR detection, with accurate, reliable results and good reproducibility.

This preservation fluid is compatible with mainstream stool DNA extraction methods. We recommend using YALI Biotech’s YALEPIC stool genomic DNA extraction kit (or similar magnetic bead/spin column kits), which is specifically optimized to remove preservatives and PCR inhibitors from the preservation fluid, yielding the highest quantity and purity of DNA for NGS library construction.

Absolute precision is not required, but a sample close to 0.5 g (approximately the size of a soybean) is recommended. Insufficient sampling may result in insufficient DNA yield. Excessive sampling (>1 g) may result in relatively insufficient preservation fluid, with the sample unable to be completely immersed, and cells in some areas may be incompletely lysed or undergo putrefaction, affecting long-term preservation. The storage tube wall has a recommended fill line for reference.

Yes. The preservation fluid formulation contains effective components such as guanidine salts, which have been validated to efficiently inactivate enveloped viruses, including coronaviruses and influenza viruses. This minimizes the risk when processing potentially infectious stool samples from clinical or environmental sources after opening, protecting laboratory personnel safety.

Although designed specifically for stool, its formulation also provides similar DNA protection for samples with complex microbial communities such as animal intestinal contents, sewage sludge, or compost. However, it may not be effective for soil (due to humic acid interference), and food samples may require additional validation due to matrix interference. Self-testing or consulting technical support is recommended.