YALEPIC^® SD Stool & Soil Genomic DNA Extraction Kit (with RNase A)

Conventional stool kits are mostly optimized only for stool, while the YALEPIC SD kit uses a stool and soil dual-matrix universal formulation. The core breakthrough is the highly efficient removal of humic acid and PCR inhibitors. This enables difficult samples such as soil, compost, and sludge to yield clean, amplifiable DNA, truly achieving one kit covering both gut microbiome and environmental metagenomics research.

Yes. Alternatively, the NGS-dedicated NT-type product YALEPIC NT Stool & Soil Genomic DNA Isolation Kit (with RNase A) can be selected. Optimized lysis recovers gDNA from hard-to-lyse species in microbial communities including Gram-positive bacteria, fungi, and archaea. The de-inhibition step ensures no humic acid residue in the extracted product, enabling direct library construction for sequencing with stable coverage and alpha diversity metrics, avoiding sequencing failure or data bias caused by incomplete purification.

Humic acid strongly inhibits Taq polymerase activity, and even trace residue can cause Ct value delay or false negatives. If the eluted DNA appears pale yellow or transparent and colorless (rather than brownish-yellow), it indicates that humic acid has been removed, and conventional PCR and qPCR can be performed without additional column purification.

Fresh samples should be immediately stored at -80 degrees C, with no more than 2 freeze-thaw cycles. For samples containing preservation fluid (such as 95% ethanol, RNAlater, stool-specific preservation fluid), this kit is compatible; simply adjust the lysis buffer ratio according to the instructions.

For low-biomass samples, increasing the sample amount (up to 1 g) is recommended. Add 0.1 mm glass beads before lysis to assist mechanical disruption, and extend lysis at 56 degrees C for an additional 10 minutes. Use elution buffer pre-warmed to 65 degrees C and reduce the elution volume to 50 microL to significantly increase the final concentration.

(1) Dual-matrix universal, one kit handles both stool and soil, simplifying procurement; (2) Outstanding inhibitor removal capability, particularly suitable for downstream qPCR detection and library sequencing; (3) Localized full-process support, from trial sample application, technical protocol optimization to data analysis, YALI Biotech application scientists provide one-on-one assistance; (4) Highly competitive pricing, in-stock ready to ship, no long lead times, suitable for urgent project initiation.