YALEPIC^® Saliva DNA Sample Storage Tube

Within 30 minutes before sampling, the subject should avoid eating, drinking, smoking, or chewing gum. Rinse the mouth with clean water to remove oral debris. After waiting 2-3 minutes, relax the cheeks to allow natural saliva secretion, gently spit into the collection funnel or directly into the storage tube until the saliva (excluding bubbles) reaches the marked scale line (approximately 2-3 ml). Tighten the cap and invert 10-15 times to ensure thorough contact between saliva and preservation fluid.

This product is specifically designed for stable room temperature preservation. After collection and mixing, samples can be stably stored at 4-37 degrees C for up to 2 years, without the need for immediate extraction. This makes it highly suitable for scenarios requiring centralized transport and batch processing after sample collection (such as multi-center clinical trials, large cohort studies). Even after months at room temperature, high-purity, intact high-molecular-weight DNA can still be extracted.

The operation is simple, with no special steps required. Transfer the mixed fluid from the tube to a centrifuge tube, centrifuge and discard the supernatant, then use any commercial saliva/blood genomic DNA extraction kit (magnetic bead or spin column method) following standard protocols. The protectant does not affect proteinase K activity, and no additional washing is needed. We recommend using YALI Biotech’s matching extraction kit for optimal results.

Absolutely. The total DNA extracted from this storage tube is typically 10-50 micrograms (depending on individual variation), with A260/280 ratio of 1.8-2.0 and A260/230 ratio >1.8. Fragment size is predominantly >=20 kb, with high integrity. We have verified its full compatibility with Illumina, MGI, and other platforms for whole-genome sequencing (WGS), target capture sequencing, and whole-exome sequencing (WES), with uniform sequencing data coverage and low GC bias.

The YALEPIC saliva DNA storage tube provides highly competitive cost-effectiveness while maintaining equivalent DNA stability performance (2 years at room temperature), suitable for large-scale procurement. Additionally, our protectant formulation is compatible with the vast majority of commercial extraction kits, with no restrictions on downstream workflows, and is specially optimized for high-temperature long-distance transport, making it more suitable for logistics conditions in China and countries along the Belt and Road with high summer temperatures. Furthermore, YALI Biotech provides full-process technical support from collection, preservation, to extraction.

Yes. If long-term sample storage (over 2 years) or special cell culture is needed, it is recommended to transfer the mixed fluid to cryovials for storage at -20 degrees C or -80 degrees C, either after 2 years of stable room temperature storage or for long-term biobanking purposes. However, we note that room temperature storage already meets the vast majority of long-term research needs, and repeated freeze-thaw may actually affect DNA integrity.

This product has passed ISO 13485 system certification and has completed practical application validation at multiple third-party genetic testing companies. YALI Biotech provides the matching YALEPIC magnetic bead-based saliva genomic DNA extraction kit, enabling high-throughput operation on automated extraction instruments. Welcome to contact us for trial sample applications.