YALEPIC^® Oral Swab Genomic DNA Extraction Kit

Absolutely. The YALEPIC Swab Genomic DNA Isolation Kit is specifically optimized for PCR applications, with significant inhibitor removal performance. The extracted DNA can be directly used as a template for conventional PCR, qPCR, and multiplex PCR. Typically, 1-2 oral swabs yield sufficient genomic DNA for 100 PCR reactions. If the amplification Ct value is high, verify that the swab sampling was adequate, or reduce the elution volume to increase DNA concentration.

Yes. This kit is compatible with various swab samples containing guanidinium isothiocyanate or non-guanidine salt-based viral transport media and cell preservation fluids. For throat or nasal swabs with preservation fluid, simply take 200-400 microL of the fluid (centrifuge to collect the pellet) and follow the standard protocol to obtain high-purity DNA. Particularly suitable for re-testing human genomic DNA in positive samples during COVID-19 and influenza virus detection.

Low DNA yield is commonly caused by insufficient cell collection on the swab, short digestion time, or low elution efficiency. Recommendations: (1) Thoroughly scrape the buccal mucosa during oral swab sampling to ensure adequate cell count; (2) Extend proteinase K digestion at 56 degrees C to 1 hour; (3) Pre-warm the Elution Buffer to 60 degrees C before elution and incubate on the membrane for 3-5 minutes. If OD260/280 is low (<1.7), protein residue may be present; add an additional wash step or re-purify.

Yes. For nasopharyngeal swab samples that may contain both pathogen DNA (such as EBV, adenovirus) and host cells, this kit can unbiasedly co-extract total DNA. The extracted product can be directly used for viral DNA quantification and human housekeeping gene detection, meeting the need for same-tube extraction and multiplex detection, making it ideal for combined pathogen screening.

Yes. This kit has good recovery capability for trace DNA and is suitable for forensic biological specimens, fingerprint wipe swabs, and exfoliated cell extraction. For extremely low copy number samples, a 20-30 microL elution volume is recommended, along with Carrier RNA, to significantly improve trace DNA recovery efficiency. The product meets STR typing requirements.

A single extraction takes approximately 25-30 minutes (excluding digestion time), and the entire process can be completed at room temperature without low-temperature centrifugation. Standard operations include: proteinase K digestion (can be pre-warmed to accelerate), binding, two washes, and elution. All steps are performed at room temperature, with maximum centrifugation speed of ~13,000 rpm. A standard benchtop centrifuge is sufficient, making it ideal for field rapid detection vehicles or primary laboratories.