YALEPIC^® NT Stool & Soil Genomic DNA Isolation Kit (with RNase A)

Yes, the extracted genomic DNA covers the complete genomes of soil bacteria (including Bacillus and Actinomyces), archaea, and fungi. The lysis process uses dual physical and chemical methods, equipped with high-density grinding beads, effectively breaking down mold hyphae and chlamydospores to ensure fungal DNA yield. Commonly used for bacterial 16S rDNA, fungal ITS, and 18S rDNA amplification and sequencing.

It is recommended to immediately store at -80 degrees C after collection or use commercial stool preservation fluid to stabilize nucleic acids. For direct extraction of fresh samples, 200 mg is sufficient; frozen samples should avoid repeated freeze-thaw, and after weighing, add directly to the lysis tube. Liquid or semi-liquid stool (such as diarrheal stool) can be collected with a sterile cotton swab and directly immersed in lysis buffer to start extraction, without freeze-drying, making the operation user-friendly.

Smearing is commonly caused by humic acid residue or excessive mechanical shearing. This kit optimizes vortex time (horizontal vortex adapter at maximum speed for 5 minutes). If smearing occurs, reduce the vortex speed to medium intensity to avoid excessive fragmentation. Low yield is mostly seen with insufficient soil sample or high sandy soil; increasing the sample amount and extending lysis time is recommended.

Slight coloration often originates from residual humic acid or stereobilin, and small amounts of pigment have no effect on conventional PCR and qPCR. If the color is dark and the ratio is low, it is recommended to increase the elution buffer temperature to 65 degrees C for elution, or add an equal-volume chloroform extraction step before the binding step as an alternative (however, the NT-type already integrates highly efficient precipitation, and most samples do not require this step).

The core design of this kit is inhibitor removal. A proprietary precipitation solution forms insoluble complexes with humic acid, bile salts, and polysaccharides for removal by centrifugation. If Ct delay still occurs, it may be due to incomplete lysis resulting in low DNA yield or trace inhibitor residue. It is recommended to first increase the template to 2 microL of eluted DNA for re-testing; if the Ct advances, it indicates insufficient template. If delay persists, dilute the DNA 1:2 with elution buffer and re-amplify; dilution can eliminate trace inhibitor interference. Under normal operation, the Ct values of stool and soil DNA extracted with this kit in qPCR deviate from theoretical values by no more than +/-0.5.