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YG28002-YALEPIC-MagEVO-Viral-DNA-RNA-Extraction-Kit

YALEPIC® MagEVO Viral DNA/RNA Extraction Kit

Clean Extraction, Clear Results — Reliable viral DNA and RNA Isolation from viruses (automated) via magnetic bead

An optimized magnetic bead workflow that turns viruses (automated) into high-quality viral DNA and RNA ready for downstream applications.

Number Specifications Catalog Price Add To Cart
YG28002 96T Online Consult +
YG28002-A (Prepackaged) 32T Online Consult +
YG28002-B (Prepackaged) 48T Online Consult +
YG28002-C (Prepackaged) 96T Online Consult +

Product Description

YALEPIC® MagEVO Viral DNA/RNA Extraction Kit can quickly extract high-purity viral nucleic acids (DNA/RNA) from various liquid samples such as blood, serum, plasma, and swab eluate. The unique embedded superparamagnetic magnetic beads can achieve the purpose of rapid separation and purification of nucleic acids.  It is suitable for isolating viral DNA/RNA from The isolated DNA/RNA with high purity can be applied in PCR, RT-PCR, qPCR, and qRT-PCR, etc. 

Features

  • High sensitivity: Efficiently detect low concentration samples and reduce the risk of missed detection.
  • Easy to operate: Automated extraction process up to 96 individual samples in 20 minutes.

Application

Up to 200 μl of  plasma, serum, whole blood, tissue homogenate, cell-free body fluid, nasopharyngeal or oropharyngeal aspirate/ wash,  bronchoalveolar lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or oropharyngeal swab and animal cell culture  supernatant.

Storage

Transport at room temperature.

Storage at 10 ~ 30°C for one year.

Can the YALEPIC MagEVO Viral DNA/RNA Extraction Kit extract both DNA and RNA simultaneously?

Yes. This kit uses a co-extraction buffer formulation, enabling simultaneous recovery of both viral DNA and RNA from a single sample in one extraction without performing two separate lysis steps, saving sample material and experimental time. It is the preferred extraction reagent for respiratory virus and mixed pathogen samples.

What are the differences among the three pre-filled models YG28002-A, B, and C, and how should I choose?

Model A (32T) is low-throughput, suitable for small labs processing occasional swab samples. Model B (48T) is designed for small-to-medium routine testing. Model C (96T) is a pre-filled plate format compatible with automated nucleic acid extractors for high-throughput batch runs, eliminating the need for manual reagent dispensing.

The kit is stored at 10-30 degrees C. Will it lose effectiveness in hot summer conditions?

The product formulation has been validated for high-temperature stability. It can be stored at 10-30 degrees C in the dark at room temperature. In summer, as long as the ambient temperature does not exceed 30 degrees C, refrigeration is not needed. Simply avoid direct sunlight to maintain normal shelf life. Room-temperature shipping will not affect reagent activity.

Can this kit extract nucleic acids from high-impurity samples such as sputum or feces?

Yes. The optimized lysis buffer can break through mucus and fecal impurities. For thick sputum, pre-dilute with saline. For fecal samples, use the supernatant for extraction to significantly improve nucleic acid yield and purity. Alternatively, YALI BIOTECH offers dedicated nucleic acid extraction kits for saliva and feces samples.

Can the extracted nucleic acids be directly used for qPCR and NGS whole-genome sequencing?

Yes. The purified nucleic acids are free of protein and salt inhibitors and can be directly used for qPCR, RT-PCR, and whole-genome next-generation sequencing without secondary purification, meeting both research and clinical testing standards.

Can the kit be used for both manual extraction and with automated nucleic acid extractors?

It is universally compatible. The standard 96T version supports manual reagent dispensing and manual extraction. The pre-filled A/B/C models are optimized for the YALEPIC Pure 32 automated magnetic bead extraction system and are also compatible with most mainstream domestic and imported nucleic acid extractors.

How long does a single batch extraction take? Can extraction be completed in as little as 20 minutes?

For simple swab and plasma samples, the complete lysis-binding-washing-elution workflow can be finished in as little as 30 minutes.

What sample volume is applicable? Is the loading volume the same for whole blood and plasma?

The recommended sample volume is generally 100-200 microL. Plasma and serum can be loaded directly. For whole blood samples, it is recommended to first separate the plasma before extraction to reduce heme impurity interference.

Do the pre-filled models A, B, and C contain pre-dispensed reagents?

Yes. All three pre-filled models come with pre-dispensed magnetic beads, lysis buffer, wash buffer, and elution buffer, eliminating the need for manual reagent aliquoting, reducing pipetting errors, and shortening pre-processing time.

What causes low nucleic acid concentration or poor yield?

Common causes include repeated freeze-thaw of samples, low viral load in the sample, and insufficient lysis mixing. Using fresh samples and thorough vortex lysis can effectively improve nucleic acid recovery.

Can the kit be used for animal disease virus nucleic acid extraction (e.g., swine fever, avian influenza)?

It is fully compatible. The kit has been extensively validated for DNA/RNA extraction from livestock and poultry serum, throat swabs, and tissue samples for animal viruses, and is widely used for farm disease screening.

After opening a full kit box, how should the remaining reagents be stored?

After opening, keep the original packaging sealed and store at 10-30 degrees C protected from light. Seal the dispensed magnetic bead solution tightly and avoid environmental RNase contamination. Use within 1 month for optimal extraction efficiency.

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