YALEPIC^® MagEVO Stool & Soil DNA Extraction Kit

In total DNA extracted from human fecal samples, the vast majority (over 80%) comes from gut microbes, with host DNA typically not exceeding 20%. The specific ratio varies depending on the sample source (human, mouse, rat, dog, cat, etc.) and individual differences.

It is recommended to process samples as soon as possible after collection. If same-day extraction is not possible, fecal samples should be frozen as soon as possible at -20 degrees C or -80 degrees C. For long-term preservation, dedicated fecal nucleic acid preservation tubes may also be considered.

Yes. The kit has been validated for fecal samples from a variety of animals, including humans, mice, rats, dogs, cats, horses, and cattle.

Fecal samples often contain PCR inhibitors such as bile salts, complex polysaccharides, and humic acids. This kit effectively removes these inhibitors through an optimized lysis system and wash protocol, ensuring that the extracted DNA can be directly used for downstream experiments such as PCR and qPCR without additional purification steps.

From approximately 0.2 g of human fecal sample, 5-25 microgram of total DNA can be extracted. From mouse or rat fecal samples (approximately 0.1 g), the extractable DNA amount typically falls within the aforementioned range.

Yes. The kit can simultaneously extract DNA from bacteria, fungi (including yeast and mold), actinomycetes, and various other microorganisms from soil samples.

Generally, 100-500 mg of soil sample is recommended, and the specific amount can be appropriately adjusted based on soil type. For soils with high humus content (e.g., humus-rich soil, wetland silt), the sample amount can be appropriately reduced. For sandy soil, the sample amount can be appropriately increased. It is recommended to conduct a small-scale pilot experiment based on soil type for initial use to determine the optimal sample amount.

Humic acids and fulvic acids in soil (especially humus-rich soil) are major inhibitors of PCR and qPCR. The unique buffer system and wash solution of this kit can efficiently remove these interfering substances. The extracted DNA is colorless and transparent, with no brown residue, and can be directly used for downstream molecular detection.

This is a normal phenomenon. If precipitate appears in the buffer (especially under low-temperature conditions), heat the buffer in a 37 degrees C water bath and mix thoroughly. Use after the precipitate has completely dissolved. Check that all reagents have returned to room temperature before use.

DNA yield from soil samples varies depending on soil type and microbial abundance. Generally, over 5 microgram of DNA can be obtained per gram of soil, with DNA fragment lengths primarily distributed between 20-30 kb.