YALEPIC^® MagEVO Saliva DNA Extraction Kit

This kit is suitable for fresh saliva samples, saliva samples with preservation fluid, and oral swab samples. The sample collection process is painless and non-invasive; subjects only need to spit an appropriate amount of saliva to complete sampling. It is suitable for batch testing in hospital laboratory departments and third-party medical testing institutes, as well as genetics research at research institutions and personnel screening at forensic identification agencies.

This kit can extract 2-10 micrograms of genomic DNA from 200-400 microL of saliva samples (varying with individual differences), with OD260/280 ratio stably between 1.8-2.0, fully demonstrating excellent product purity. The extracted genomic DNA can be directly used for qPCR quantitative analysis, NGS library construction, genotyping, STR analysis, gene chip detection, and other downstream experiments, without PCR inhibitor residue interference.

The manual operation workflow (including all steps of sample lysis digestion, magnetic bead binding, washing, and elution) takes approximately 60 minutes. Using an automated nucleic acid extraction instrument, extraction time for 96 samples per run is only 40 minutes. Compared to the 2-3 hours of the traditional phenol-chloroform method, this product significantly shortens the experimental cycle and improves laboratory efficiency.

This kit uses magnetic bead purification technology, which has three significant advantages over the traditional silica membrane spin column method: First, no centrifugation needed – magnetic separation technology completely eliminates the risk of spin column clogging, particularly suitable for processing high-viscosity saliva samples. Second, excellent automation compatibility – can seamlessly interface with various nucleic acid extraction instruments for high-throughput unattended automated extraction. Third, superior yield and purity – the large surface area of the magnetic beads ensures maximal DNA binding efficiency, and the washing steps thoroughly remove inhibitors, guaranteeing product quality.

The high-purity genomic DNA extracted with this kit can be widely used for real-time qPCR (for gene expression analysis and pathogen detection), next-generation sequencing NGS (for whole-genome sequencing, targeted sequencing, and exome sequencing), gene chips (for SNP genotyping and genome-wide association analysis), STR typing (for forensic individual identification and paternity testing), and molecular cloning (for restriction digestion and ligation and other routine molecular biology operations).