YALEPIC^® MagEVO Blood DNA Extraction Kit

This kit is suitable for various blood sample types, including: freshly collected anticoagulated whole blood (EDTA anticoagulant, sodium citrate anticoagulant, heparin anticoagulant), frozen preserved anticoagulated blood samples, blood clots, and buffy coat samples. Regardless of whether the sample is freshly collected or has been long-term frozen stored, high-quality genomic DNA can be obtained. A starting volume of 50 microL to 1 mL of blood sample is recommended for extraction, and the specific starting volume can be adjusted based on downstream experimental needs.

This kit uses unique nano-magnetic bead separation technology and an optimized buffer system. The extracted genomic DNA is of high purity, with OD260/280 ratio between 1.8-2.0 and OD260/230 ratio >1.8, effectively removing PCR inhibitors such as hemoglobin and heparin. DNA fragment integrity can reach 50 kb, and yield is stable and reliable (5-15 microgram of high-quality genomic DNA can be extracted from 200 microL of whole blood). The extraction product fully meets the requirements of downstream experiments with high DNA quality and purity demands, such as next-generation sequencing (NGS) library construction, qPCR, and Southern blot hybridization.

Yes, the YALEPIC MagEVO series kits are available in bottle and pre-filled specifications (32T/48T/96T pre-filled), perfectly compatible with mainstream magnetic bead-based automated nucleic acid extractors, matching instruments such as the YALI BIOTECH YALEPIC Pure 32 Automated Nucleic Acid Extractor CAT#MC-A-32-16. The entire extraction process is based on the magnetic bead separation principle, without the need for centrifugation or vacuum filtration. All steps of lysis, binding, washing, and elution are completed on the automated equipment, greatly reducing manual operational errors and suitable for high-throughput sample processing scenarios.

Using the YALEPIC MagEVO magnetic bead-based blood DNA extraction kit, the manual operation portion is very simple (only requiring sample addition and simple magnetic rack separation steps), and the entire workflow can be completed within 1 hour. If used with an automated nucleic acid extractor, in 96 deep-well plate mode, a single extraction (including lysis, binding, washing, and elution) takes only 35-45 minutes, significantly improving laboratory work efficiency. The entire process does not require toxic organic solvents such as phenol/chloroform, ensuring safe and environmentally friendly operation.

The high-quality genomic DNA extracted with this kit can be widely used in the following molecular biology experiments: qPCR and conventional PCR – for gene expression analysis, pathogen detection, genotyping, etc. Next-generation sequencing (NGS) – library construction, whole genome sequencing, targeted sequencing, metagenomic sequencing. Gene cloning and vector construction – restriction enzyme digestion, ligation and transformation. Southern blot hybridization – gene copy number analysis, transgene detection. Chip detection – gene chip hybridization, SNP genotyping. Restriction enzyme analysis – restriction fragment length polymorphism (RFLP) analysis. The extraction product requires no further purification and can be directly used as template in the above experiments.

The YALEPIC MagEVO magnetic bead-based blood DNA extraction kit supports ambient-temperature shipping (10-30 degrees C) without cold-chain logistics, effectively reducing transportation costs. Upon arrival, store the product at 10-30 degrees C room temperature protected from light; do not freeze. Under proper storage conditions, the product shelf life is 12 months from the date of production. After opening, use as soon as possible and cap the bottles tightly after use to avoid reagent contamination or component evaporation.

This kit uses an optimized low-salt elution buffer in the elution step, minimizing magnetic bead carryover and ensuring that the eluted DNA product does not contain components that inhibit PCR reactions (such as high salt, EDTA carryover, etc.). Additionally, the wash steps of the kit are specially optimized to effectively remove PCR inhibitors such as hemoglobin, heparin, and proteins. The extracted DNA product can be directly used for PCR and qPCR reactions without additional purification steps. If your downstream experiments are highly sensitive to inhibitors, it is recommended to appropriately dilute the DNA template before performing the amplification reaction.

The YALEPIC MagEVO series focuses on blood genomic DNA extraction and is particularly suitable for extracting high-molecular-weight genomic DNA from whole blood samples. If you need to extract viral DNA/RNA or cell-free DNA (cfDNA/cfRNA) from blood, YALI BIOTECH offers dedicated YALEPIC series viral DNA/RNA extraction kits and cell-free DNA extraction kits, respectively optimized for low-abundance pathogen nucleic acids and trace cfDNA samples. Please contact us through our official channels for consultation and purchase.

You can choose the appropriate extraction method based on experimental scale: Manual extraction (low throughput) – only a magnetic rack is needed (can be purchased separately or self-provided), with no other special equipment required. It is recommended to use nuclease-free centrifuge tubes and pipette tips. Automated extraction (high throughput) – a magnetic bead-based automated nucleic acid extractor is required, along with supporting automated consumables such as 96-well deep-well plates and magnetic rod sleeves.

If you encounter low yield or unsatisfactory purity, troubleshoot according to the following steps: Sample issues – check whether the blood sample is fresh and whether the correct anticoagulant (EDTA or sodium citrate recommended) was used, and whether the sample has undergone repeated freeze-thaw. Lysis step – confirm that the lysis buffer has been thoroughly mixed and that lysis time and temperature meet the manual’s requirements. Magnetic bead separation – confirm that the magnetic rack is functioning properly and that magnetic beads have been fully adsorbed and collected. Wash steps – confirm that the wash buffer has been prepared and used as required and that the number of washes is sufficient. Elution step – confirm that the elution buffer has been preheated (65 degrees C preheating recommended) and that the elution time is sufficient (5-10 minutes recommended).