YALEPIC® MagEVO PCR And Gel DNA Extraction Kit

Yes. The YALEPIC MagEVO magnetic bead-based PCR product/agarose gel DNA recovery kit is specially designed as a dual-use kit – it is suitable both for recovering target DNA fragments from TAE/TBE agarose gels (gel recovery mode) and for directly purifying DNA from PCR reaction systems, restriction digestion systems, ligation reaction systems, and other solutions (purification mode). Recovery efficiency in both modes exceeds 85%, significantly reducing laboratory kit procurement costs and inventory pressure.

The core differences lie in the solid-liquid separation method and automation compatibility: Spin column method – relies on high-speed centrifugation to bind DNA to silica membranes, requiring multiple centrifugation steps with time-consuming manual operation, and is difficult to adapt for high-throughput automated equipment. Magnetic bead method (this product) – uses rapid response of nano-magnetic beads in a magnetic field for solid-liquid separation, requiring no centrifuge, with simpler operation, and can easily integrate with liquid handling workstations, magnetic rod automated extraction systems, and 96-channel automated platforms, making it especially suitable for high-throughput, large-sample-volume laboratory scenarios.

This kit can efficiently recover DNA fragments above 80 bp, with recovery efficiencies for different fragment sizes as follows: 50-80 bp: approximately 50-60%; 80 bp-10 kb: >=85%; 10-20 kb: approximately 60-75%. In gel recovery mode, it is recommended to cut gel bands quickly to minimize UV damage to DNA and avoid prolonged exposure that reduces recovery efficiency.

Yes. This product is specially designed for use with automated workstations or magnetic rod automated magnetic bead extraction systems, compatible with mainstream automated extraction platforms (e.g., KingFisher Flex, Tecan, Hamilton, YALEPIC, etc.). It is available in 96T standard and 32T/48T/96T pre-filled plate versions. The pre-filled version has components pre-dispensed into 96-well deep-well plates, ready to use out of the box, significantly reducing manual preparation time. Whether for batch gel recovery in clone screening, PCR product purification in NGS library construction, or genotyping sample preparation in high-throughput screening, fully automated efficient operation can be achieved.

Stability is very reliable. This kit supports room-temperature shipping and storage at 10-30 degrees C without cold chain. Under room temperature, protected from light, and sealed conditions, the shelf life is up to 18 months. All buffer components have undergone rigorous room-temperature stability validation; even during 7-day summer shipping, performance stability is guaranteed. The room-temperature storage feature makes it especially suitable for labs with limited cold chain conditions, such as primary labs, field sampling bases, and third-party testing facilities.

The purified DNA can be directly used for a variety of downstream molecular biology experiments, including Sanger sequencing, next-generation sequencing (NGS) library construction, PCR/qPCR amplification, restriction enzyme digestion (restriction enzymes/ligases), TA cloning/homologous recombination, DNA labeling and probe preparation, in vitro transcription, and microarray analysis.