YALEPIC® MagEVO Cell-Free DNA Extraction Kit (Small Volume)

Compatible with plasma, serum, amniotic fluid, and other cell-free body fluids; standard starting sample volume is 0.2-1 mL, compatible with blood samples collected in EDTA conventional blood collection tubes and Streck cfDNA-dedicated stabilizer tubes.

Fully supports automated extraction, compatible with 32-channel and 96-channel mainstream nucleic acid extraction instruments. Optimized pre-loaded reagent versions (Type A / Type B / Type C) are also available, requiring no manual preparation, for direct instrument use.

The purified product has no protein, salt ion, or other inhibitor residues, and can be directly used for qPCR quantification, NGS next-generation sequencing, library construction, non-invasive prenatal testing, tumor liquid biopsy, methylation analysis, and other experiments.

It is not an extraction failure. cfDNA is naturally present at extremely low levels in plasma and is a trace nucleic acid. Nanodrop UV detection has insufficient sensitivity and tends to show lower values. Using the Qubit high-sensitivity fluorometer for concentration detection is recommended for more accurate results.

Fresh plasma is recommended for short-term storage at 4 degrees C after separation; for long-term storage, aliquot and store at -20 degrees C, avoiding repeated freeze-thaw. If blood samples cannot be processed promptly, cfDNA-dedicated stabilizer blood collection tubes are recommended to prevent blood cell lysis releasing genomic DNA contamination.

No cold chain needed; room temperature transport is supported. Store sealed at 10-30 degrees C room temperature in the dark. The shelf life is stable when unopened. Avoid placing in damp, cold, or environments above 35 degrees C.

This kit is specifically optimized for short-fragment cfDNA. The proprietary nano-magnetic beads efficiently capture small-fragment nucleic acids, with uniform fragment coverage and no selection bias, maximally preserving the original nucleic acid information of the sample.

The standard YM26004 is a standard reagent that can be self-configured for manual or instrument use; YM26004-A/B/C are pre-loaded versions, pre-dispensed to match instrument consumables, ready to use, eliminating preparation steps, suitable for high-throughput batch experiments. Choose Type A for 32T, Type B for 48T, and Type C for 96T.

The entire process requires no phenol, chloroform, beta-mercaptoethanol, or other toxic organic solvents. Purification relies on magnetic bead adsorption plus proprietary wash buffer, making the operation safe and the experimental environment more friendly.

Eluted cfDNA can be stored at 4 degrees C for 1-3 days short-term; for long-term storage, aliquot and store at -20 degrees C or -80 degrees C, avoiding repeated freeze-thaw to prevent nucleic acid degradation that could affect downstream experimental results.