YALEPIC® Bacteria Genomic DNA Isolation Kit (No RNase A)

This kit is universally applicable to all routine research strains of Gram-negative and Gram-positive bacteria, meeting the genomic DNA extraction needs of most common bacterial samples in laboratories.

It does not affect the basic extraction performance. The kit includes an optimized buffer system that naturally removes most RNA impurities. If complete removal of RNA residue is required, standard RNase A can be added separately, providing flexibility to meet different experimental standards.

No. Using an improved silica membrane spin column method, the entire process avoids harmful organic solvents such as phenol and chloroform, and eliminates ethanol precipitation steps. The experiment is safe, non-polluting, and the workflow is more streamlined.

The standard single preparation accommodates 10^6 to 10^8 bacterial cells, with a maximum capacity of 2.0 x 10^9 Gram-negative or Gram-positive bacterial cells, meeting the extraction needs of routine and medium-to-high concentration bacterial culture samples.

The extracted high-purity, intact genomic DNA can be directly used for restriction digestion, conventional PCR amplification, real-time qPCR, blot hybridization, gene library construction, bacterial strain sequencing, and other molecular biology experiments.

Following the standard protocol, the complete DNA extraction workflow including lysis, binding, washing, and elution for multiple bacterial samples can be completed within 1 hour, suitable for batch sample processing.

Room temperature transport is supported without cold chain requirements. For daily storage, place in a dry, dark environment at 10-30 degrees C room temperature. Storage conditions are flexible and the kit is not prone to failure.

The kit’s proprietary buffer system maximally removes contaminating proteins, lipids, and inhibitory impurities. Strictly follow the protocol to control the bacterial sample input amount and ensure thorough cell lysis to guarantee high DNA purity without experimental inhibition.

No. This kit is specifically designed for bacterial cell wall lysis and is not compatible with eukaryotic microorganisms such as yeast and fungi. Such samples require a dedicated fungal genomic DNA extraction kit.