YALEPIC^® Blood RNA Isolation Kit

The YALEPIC Blood RNA Extraction Kit is suitable for fresh whole blood from humans, mice, rats, rabbits, pigs, cattle, sheep, and other mammals. Compatible with EDTA, citrate, heparin, and other common anticoagulants, covering clinical testing, basic research, and veterinary needs.

The recommended single processing volume is up to 1.5 mL of whole blood (for larger volumes, consult YALI Biotech technical team). Within this range, stable RNA yield and purity are achieved. For larger volumes, split and process in batches to ensure thorough lysis and stable RNA quality.

NanoDrop testing shows A260/A280 typically >=1.9 and A260/A230 >=1.5, indicating high purity with effective removal of heme, heparin, and other PCR inhibitors. The extracted RNA can be directly used for RT-PCR, Real-Time RT-qPCR, and other experiments without additional purification.

This product (catalog YR23003) does not contain DNase I. For downstream experiments sensitive to genomic DNA residue (e.g., RT-qPCR quantitative analysis), we recommend using YALI Biotech DNase I (sold separately) for on-column or off-column DNase digestion after RNA extraction to thoroughly remove genomic DNA contamination.

No. The YALEPIC Blood RNA Extraction Kit uses column-based purification technology, with no phenol, chloroform, or other toxic organic reagents needed. The operation is safe and environmentally friendly, compliant with modern green laboratory standards, especially suitable for researchers who perform frequent extractions.

Yes. The extracted blood total RNA has good integrity (clear 28S/18S rRNA bands on agarose gel) and high purity, fully meeting the requirements of RNA-Seq high-throughput sequencing, microarray analysis, and other high-quality-RNA downstream experiments.

All components can be stored at 10-30 degrees C room temperature, no refrigeration or freezing needed. Room temperature transport without dry ice or ice packs effectively reduces logistics and storage costs. Store sealed and protected from light; use promptly after opening.

For best RNA quality, extract within 2 hours of blood collection. If immediate processing is not possible, anticoagulated whole blood can be temporarily stored at 2-8 degrees C (no more than 24 hours), or white blood cells can be separated and stored at -80 degrees C. For long-term storage, use YALI Biotech blood RNA sample storage tubes for room temperature stabilization.

The blood RNA extraction kit is specifically optimized for whole blood, with a lysis system that thoroughly lyses blood cells and effectively removes heme and other blood-specific inhibitors. The tissue RNA extraction kit is designed for solid tissue mechanical homogenization and protein removal. Choose the appropriate kit based on sample type for best results.

Yes. YALI Biotech supports bulk procurement and OEM/ODM customization, including different specifications, custom labels, and technical support. For large-volume or customization needs, contact us via the website or call 0512-66256010 for a dedicated quote.