YALEPIC® MagEVO Pathogenic Microbes DNA/RNA Extraction Kit

This kit is suitable for various clinical and environmental samples, including: fresh or frozen anticoagulated whole blood (<=400 microL), nasopharyngeal swabs, sputum, bronchoalveolar lavage fluid, cerebrospinal fluid, pleural or ascitic fluid and other body fluid samples (2-3 ml, <=5x10^6 cells), bacterial (<=1x10^9 cells) and fungal (<=1x10^9 cells) microbial cultures, and also supports DNA/RNA extraction from stool samples (100 mg solid or 200 microL stool in preservation medium).

Yes. The kit is specifically optimized for co-extraction of pathogenic microbial DNA and RNA, capable of simultaneously extracting and purifying DNA and RNA from viruses, bacteria, and fungi in the same sample. This feature is particularly suitable for metagenomic sequencing and pathogen co-detection applications requiring simultaneous DNA virus and RNA virus detection.

This kit uses a lysis system combining chemical lysis and mechanical grinding, with lysis optimization for fungi (such as yeast, Candida) and Gram-positive bacteria with thick cell walls that are difficult to lyse. It can efficiently release nucleic acids, significantly improving the detection rate of such microorganisms, suitable for low-abundance pathogen detection in clinical samples.

The purified DNA/RNA products have high purity, good integrity, and no protein or nuclease residue, suitable for various downstream molecular biology applications, including: conventional PCR and real-time quantitative PCR (qPCR), metagenomic library construction (mNGS), whole-genome sequencing (WGS), 16S rDNA-based microbiome analysis, and shotgun metagenomic sequencing analysis.

Yes. This kit uses magnetic bead-based technology, compatible with both manual extraction using a magnetic rack and fully compatible with various magnetic rod-based and pipetting-based automated nucleic acid extraction instruments for high-throughput extraction, suitable for large-scale sample processing needs in clinical testing centers, CDC, and research laboratories.

No. This kit uses nano-magnetic bead purification technology, requiring no phenol, chloroform, or other toxic organic solvents throughout the process. The extraction process does not involve organic reagent extraction steps, making it safer for laboratory personnel and compliant with green environmental laboratory practices.

This kit supports ambient temperature transport, requiring no cold chain throughout. All components can be stored at room temperature (10-30 degrees C), with a shelf life of 12 months. These storage conditions greatly reduce cold chain costs during transport and storage, facilitating laboratory inventory and daily use.

The MagEVO kit is optimized for high yield and good reproducibility. The purified DNA/RNA products typically have an A260/A280 ratio between 1.8-2.0, with low protein residue. The purified products are free of PCR inhibitors and nucleases, and can be directly used for downstream amplification and sequencing reactions without additional purification.

Yes. This kit has undergone specific buffer optimization for sputum, bronchoalveolar lavage fluid, and other complex body fluid samples rich in mucin and various PCR inhibitors. Combined with efficient washing steps, it can effectively remove inhibitors from samples, ensuring that the extracted nucleic acid purity meets downstream detection requirements.

mNGS has high requirements for nucleic acid extraction purity, integrity, and background bacterial contamination control. This kit uses an efficient washing system that maximally removes host nucleic acid interference and background bacterial contamination, with purified products suitable for mNGS library construction. Use NGS-grade water and nuclease-free consumables during mNGS experiments, and set up negative controls during extraction to monitor background contamination.