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YM26003-YALEPIC-MagEVO-Animal-Cell-Tissue-DNA-Extraction-Kit-1

YALEPIC® MagEVO Tissue DNA Extraction Kit

Reliable Isolation, Reproducible Data — Reliable genomic DNA Isolation from animal / solid tissue via magnetic bead

An optimized magnetic bead workflow that turns animal / solid tissue into high-quality genomic DNA ready for downstream applications.

Number Specifications Catalog Price Add To Cart
YM26003 96T Online Consult +
YM26003-A (Prepackaged) 32T Online Consult +
YM26003-B (Prepackaged) 48T Online Consult +
YM26003-C (Prepackaged) 96T Online Consult +

Product Description

YALEPIC® MagEVO Tissue DNA Extraction Kit employs unique separation properties and a specialized buffer system to extract genomic DNA from animal tissues.  The high-affinity magnetic beads used in the kit can adsorb nucleic acids in a high-salt buffer and release nucleic acids in a low-salt elution buffer, thereby achieving rapid separation and purification of nucleic acids. The purified DNA is suitable for quantitative PCR, gene cloning, NGS, blot hybridization, and automated extraction on high-throughput workstations.

Features

  • Simple and fast: Extract genomic DNA from  samples within 40 min.
  • High yield and good integrity: Highly efficient extraction of intact genomic DNA.
  • Automated extraction: This kit can be used with automated nucleic acid extraction instrument for high-throughput extraction.

Application

25mg~50mg fresh/frozon tissue samples.

Storage

Transport at room temperature.

Storage at 10 ~ 30°C for one year.

If ambient temperatures often exceed 30°C, we suggest storing Proteinase K and Magbeads PM at 2 ~ 8°C (protect from freezing).

What sample types is this magnetic bead-based DNA extraction kit suitable for?

It is primarily suitable for fresh animal tissues (mouse tail, muscle, viscera, etc., 10-30 mg) and routine cultured cells (<=1x10^6 cells). It is not suitable for paraffin-fixed tissues or blood samples. For other sample types, please consult customer service for recommendations.

Can the DNA extracted with this kit be directly used for high-throughput sequencing?

Yes. The genomic DNA extracted with this kit has intact fragments and high purity, with a 260/280 ratio stably between 1.8-2.0, free of impurity contamination, and can be directly used for metagenomic library construction, whole genome sequencing, qPCR, and other high-end experiments.

Does it support automated high-throughput extraction instruments?

Yes. It is compatible with mainstream pipetting-based automated nucleic acid extractors and magnetic rod-based automated extraction workstations. The 32T/48T/96T pre-filled models can be directly loaded onto instruments without manual reagent preparation, suitable for batch sample testing.

Does the kit require cold chain for storage and shipping?

No. The product supports ambient-temperature shipping. For daily storage, simply keep it at 10-30 degrees C room temperature protected from light, without freezing or refrigeration, with relaxed storage conditions.

How long does the entire extraction workflow take? Is the operation complex?

The operation is extremely simple. Samples are directly lysed without cumbersome incubation, and the entire DNA extraction and purification can be completed within 1 hour. Even novices can quickly get started without professional laboratory training.

How many specifications does the kit have? Can it be purchased in small batches?

Four specifications are available: 32T pre-filled A, 48T pre-filled B, 96T standard/pre-filled C. Small-batch purchasing is supported, and bulk customization quotes are also available, meeting the throughput needs of different laboratories.

What causes substandard DNA purity and protein contamination?

The most likely causes are excessive sample input, incomplete lysis, or improper wash step operation. Strictly follow the manual to control tissue sample input at 10-30 mg, vortex thoroughly for lysis, and complete the wash steps properly to avoid contamination issues.

What are the advantages compared with traditional spin column DNA extraction kits?

Compared with spin column methods, the magnetic bead method is more compatible with automated high-throughput operations with no risk of column clogging. No toxic phenol/chloroform reagents are needed, making it safer. It requires less time and provides better DNA recovery and integrity, suitable for routine extraction of large sample batches.

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