YALEPIC®MagEVO cfDNA Maxi Extraction Kit

Standard compatibility with 1-5 ml of plasma, serum, amniotic fluid, urine, and other cell-free body fluids. This is a dedicated kit for large-volume cfDNA extraction, meeting high-sample-volume enrichment needs.

Fully compatible. The product is specifically optimized for short-fragment cfDNA, with high short-fragment proportion, no amplification bias, high nucleic acid purity, and no genomic DNA contamination. It can be directly used for NGS library construction, high-throughput sequencing, and liquid biopsy experiments.

Supports both manual extraction and fully automated high-throughput workstation dual modes; A/B/C three pre-loaded reagent versions are also provided, requiring no manual system preparation, directly compatible with mainstream automated extraction equipment for more efficient batch processing.

Yes. The kit’s buffer system is compatible with plasma, serum, amniotic fluid, urine, and various cell-free body fluids. It effectively removes urine impurities and amniotic fluid protein interference, stably purifying high-quality cell-free DNA.

No cold chain needed. Room temperature transport is supported, and daily storage at 10-30 degrees C room temperature in the dark is sufficient. No refrigeration or freezing required, making storage and use more convenient.

(1) Use cfDNA-dedicated blood collection tubes, and separate plasma as soon as possible after blood collection to avoid leukocyte lysis releasing gDNA contamination; (2) Appropriately increase the starting sample volume to 3-5 ml; (3) Strictly follow the protocol for lysis and binding steps, thoroughly mix and incubate magnetic beads, and slightly increase the elution volume to increase recovery.

Eluted cfDNA is recommended for short-term storage at 4 degrees C; for long-term storage, aliquot and store at -20 degrees C, avoiding repeated freeze-thaw to maximally maintain nucleic acid integrity.

The standard version consists of bulk reagents requiring self-preparation of the system; A/B/C pre-loaded versions are ready-to-use pre-formulated reagents, pre-dispensed in plates, for direct instrument use, saving experiment preparation time and more suitable for high-throughput automated experiments.

No. The kit uses a mild lysis magnetic bead purification method, entirely free of phenol, chloroform, beta-mercaptoethanol, and other toxic organic solvents. The experiment is safe and non-polluting.