YALEPIC^® Endotoxin Free Plasmid DNA Maxi Isolation Kit (PLUS) (100-300ml)

YALEPIC^® Endotoxin Free Plasmid DNA Maxi Isolation Kit (PLUS) (100-300ml) is suitable for extraction from 100 – 300 ml of bacterial solution cultured overnight, using an improved SDS-alkaline lysis method to lyse the bacteria. The PB solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation.  A newly developed endotoxin removal solution YEB  and separated by centrifugation to remove endotoxins  reducing endotoxin levels to as low as 0.1 EU/μg. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.

• Transfection level : Extremely low endotoxin content (< 0.1 EU/μg DNA)
• High yield: The yield of high copy plasmid is 2 mg~3 mg
• Wide compatible: The input amount of bacterial cultures is 100 – 300 ml 

100~300ml of overnight bacterial culture.

Store at 10 ~ 30°C for one year and transport at room temperature.

If ambient temperatures often exceed 30°C, we suggest storing RNase A at 2 ~ 8°C.

After addition of RNase A, Buffer PA is stable for 6 months when stored at 2 ~ 8°C.